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Discrete typing units of Trypanosoma cruzi identified in rural dogs and cats in the humid Argentinean Chaco

Published online by Cambridge University Press:  12 October 2012

G. F. ENRIQUEZ ,
M. V. CARDINAL ,
M. M. OROZCO ,
L. LANATI ,
A. G. SCHIJMAN  and
R. E. GÜRTLER
G. F. ENRIQUEZ
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
M. V. CARDINAL*
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
M. M. OROZCO
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
L. LANATI
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
A. G. SCHIJMAN
Affiliation:
Laboratorio de Biología Molecular de la Enfermedad de Chagas, Instituto de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Vuelta de Obligado 2490, Buenos Aires, Argentina
R. E. GÜRTLER
Affiliation:
Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Buenos Aires, Argentina
*
*Corresponding author: Laboratorio de Eco-Epidemiología, Departamento de Ecología, Genética y Evolución, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina. Tel/Fax: +54 11 4576 3318. E-mail: mvcardinal@ege.fcen.uba.ar
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Summary

The discrete typing units (DTUs) of Trypanosoma cruzi that infect domestic dogs and cats have rarely been studied. With this purpose we conducted a cross-sectional xenodiagnostic survey of dog and cat populations residing in 2 infested rural villages in Pampa del Indio, in the humid Argentine Chaco. Parasites were isolated by culture from 44 dogs and 12 cats with a positive xenodiagnosis. DTUs were identified from parasite culture samples using a strategy based on multiple polymerase-chain reactions. TcVI was identified in 37 of 44 dogs and in 10 of 12 cats, whereas TcV was identified in 5 dogs and in 2 cats –a new finding for cats. No mixed infections were detected. The occurrence of 2 dogs infected with TcIII –classically found in armadillos– suggests a probable link with the local sylvatic transmission cycle involving Dasypus novemcinctus armadillos and a potential risk of human infection with TcIII. Our study reinforces the importance of dogs and cats as domestic reservoir hosts and sources of various DTUs infecting humans, and suggests a link between dogs and the sylvatic transmission cycle of TcIII.

Keywords

Chagas diseaseTrypanosoma cruziDiscrete Typing Unitsdogscatsreservoir
Type
Research Article
Information
Parasitology , Volume 140 , Issue 3 , March 2013 , pp. 303 - 308
Copyright
Copyright © Cambridge University Press 2012

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