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Dynamic changes of DNA sequences in Schistosoma mansoni in the course of development

Published online by Cambridge University Press:  06 April 2009

T. Nara ,
Y. Iwamura ,
M. Tanaka ,
Y. Irie  and
K. Yasuraoka
T. Nara
Affiliation:
Institute of Basic Medical Sciences, University of Tsukaba, Ibaraki 305, Japan
Y. Iwamura
Affiliation:
Institute of Basic Medical Sciences, University of Tsukaba, Ibaraki 305, Japan
M. Tanaka
Affiliation:
Institute of Basic Medical Sciences, University of Tsukaba, Ibaraki 305, Japan
Y. Irie
Affiliation:
Institute of Basic Medical Sciences, University of Tsukaba, Ibaraki 305, Japan
K. Yasuraoka
Affiliation:
Institute of Basic Medical Sciences, University of Tsukaba, Ibaraki 305, Japan
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Summary

Deletion and/or amplification of DNA sequences in Schistosoma mansoni were demonstrated by Southern blot analysis. Total cellular DNAs and genomic clones derived from S. mansoni miracidia, adult males and females were used as probes. EndonucleaseBamHI-restricted DNAs from miracidia, adult males and females of both S. mansoni and S. japonicum were reacted to each probe. Hybridization with a total cellular DNA from S. mansoni miracidia as a probe showed elimination of signals in S. mansoni adults. On the other hand, blot analysis using a total cellular DNA from S. mansoni adult males as a probe revealed elimination of hybridization signals in S. mansoni miracidia.Hybridization with a clone SmE15 DNA from S. mansoni miracidia as a probe showed no signal in the DNAs from 5. mansoni adults, indicating these sequences deleted in adults. Hybridization experiments using the probes SmF25 and SmM51 which are 13 and 2-2 kb fragments cloned from S. mansoni adult females and males respectively, demonstrated no signal to DNA from S. mansoni miracidia.Our data suggested the existence of stage-specific DNA sequences in 5. mansoni. We propose a model for multiple-step rearrangement of DNA sequences in S. mansoni during the course of development.

Keywords

schistosomeslife-cycleDNAgenomic clonesrearrangement
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 241 - 245
Copyright
Copyright © Cambridge University Press 1990

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