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The dynamics of ultrastructural changes during Entamoeba invadens encystation

Published online by Cambridge University Press:  14 July 2020

Eman Abdelazeem Abuelwafa Mousa ,
Miako Sakaguchi ,
Risa Nakamura ,
Osama Hussein Abdella ,
Hiroki Yoshida ,
Shinjiro Hamano  and
Fumika Mi-ichi Open the ORCID record for Fumika Mi-ichi [Opens in a new window]
Eman Abdelazeem Abuelwafa Mousa
Affiliation:
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, Egypt
Miako Sakaguchi
Affiliation:
Central Laboratory, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan
Risa Nakamura
Affiliation:
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan
Osama Hussein Abdella
Affiliation:
Department of Medical Parasitology, Faculty of Medicine, South Valley University, Qena, Egypt
Hiroki Yoshida
Affiliation:
Division of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga849-8501, Japan
Shinjiro Hamano*
Affiliation:
Department of Parasitology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan The Joint Usage/Research Center on Tropical Disease, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki852-8523, Japan
Fumika Mi-ichi*
Affiliation:
Division of Molecular and Cellular Immunoscience, Department of Biomolecular Sciences, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga849-8501, Japan
*
Authors for correspondence: Fumika Mi-ichi, E-mail: fumika@cc.saga-u.ac.jp, Shinjiro Hamano, E-mail: shinjiro@nagasaki-u.ac.jp
Authors for correspondence: Fumika Mi-ichi, E-mail: fumika@cc.saga-u.ac.jp, Shinjiro Hamano, E-mail: shinjiro@nagasaki-u.ac.jp
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Abstract

Entamoeba histolytica infection causes amoebiasis, which is a global public health problem. The major route of infection is oral ingestion of E. histolytica cysts, cysts being the sole form responsible for host-to-host transmission. Cysts are produced by cell differentiation from proliferative trophozoites in a process termed ‘encystation’. Therefore, encystation is an important process from a medical as well as a biological perspective. Previous electron microscopy studies have shown the ultrastructure of precysts and mature cysts; however, the dynamics of ultrastructural changes during encystation were ambiguous. Here, we analysed a series of Entamoeba invadens encysting cells by transmission electron microscopy. Entamoeba invadens is a model for encystation and the cells were prepared by short interval time course sampling from in vitro encystation-inducing cultures. We related sampled cells to stage conversion, which was monitored in the overall population by flow cytometry. The present approach revealed the dynamics of ultrastructure changes during E. invadens encystation. Importantly, the results indicate a functional linkage of processes that are crucial in encystation, such as glycogen accumulation and cyst wall formation. Hence, this study provides a reference for studying sequential molecular events during Entamoeba encystation.

Keywords

Cell differentiationdormancyinfectious diseaseprotozoan parasite
Type
Research Article
Information
Parasitology , Volume 147 , Issue 12 , October 2020 , pp. 1305 - 1312
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Copyright
Copyright © The Author(s) 2020. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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