Skip to main content

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 7
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Uemura, Akiko Watarai, Shinobu Kushi, Yasunori Kasama, Takeshi Ohnishi, Yoshihiro and Kodama, Hiroshi 2006. Analysis of neutral glycosphingolipids from Trypanosoma brucei. Veterinary Parasitology, Vol. 140, Issue. 3-4, p. 264.
    Tsujimura, Yoshie Watarai, Shinobu Uemura, Akiko Ohnishi, Yoshihiro and Kodama, Hiroshi 2005. Effect of anti-ganglioside antibody in experimental Trypanosoma brucei infection in mice. Research in Veterinary Science, Vol. 78, Issue. 3, p. 245.
    Kaneshiro, Edna 2004. Pneumocystis Pneumonia, Third Edition. Vol. 20042646, Issue. , p. 539.
    Kaneshiro, Edna S. and Wyder, Michael A. 2000. C27 to C32 sterols found in Pneumocystis, and opportunistic pathogen of immunocompromised mammals. Lipids, Vol. 35, Issue. 3, p. 317.
    Kaneshiro, Edna S. 1998. Pneumocystis carinii pneumonia: the status of Pneumocystis biochemistry. International Journal for Parasitology, Vol. 28, Issue. 1, p. 65.
    FLORIN-CHRISTENSEN, MONICA FLORIN-CHRISTENSEN, JORGE and KANESHIRO, EDNA S. 1995. Uptake and Metabolism of L-Serine by Pneumocystis carinii carinii. The Journal of Eukaryotic Microbiology, Vol. 42, Issue. 6, p. 669.
    Su, M.D, Tony H. and Martin II, M.D, William J. 1994. PATHOGENESIS AND HOST RESPONSE IN Pneumocystis carinii PNEUMONIA. Annual Review of Medicine, Vol. 45, Issue. 1, p. 261.
    ×
  • Get access
    Add to cart USD35.00
    Check if you have access via personal or institutional login
    Log in Register Recommend to librarian

Evidence for the existence of ganglioside molecules on Pneumocystis carinii from human lungs

  • M. Sorice (a1), L. Lenti (a2), R. Misasi (a2), C. Contini (a3), L. Cignarella (a3), T. Griggi (a1), V. Vullo (a3) and C. Masala (a1)...
Extract

This study was undertaken to assess whether glycolipid antigens (particularly gangliosides) are associated with Pneumocystis carinii obtained from human lungs. Gangliosides were extracted, purified in high performance thin-layer chromatography and stained with resorcinol. Two resorcinol-positive bands, co-migrating with GM1 and GD1a were demonstrated, suggesting the existence of ganglioside molecules on P. carinii. No resorcinol-positive bands were revealed in the pulmonary control tissue. In addition, an antiserum obtained from rabbits immunized with P. carinii antigen reacted with gangliosides GM1, and GD1a, as revealed by a dot immunobinding assay. This reactivity was inhibited by first incubating the antiserum with ganglioside micelles. Furthermore, anti-glycosphingolipid antibodies (aGM1) reacted with the bands of 200 and 55 kDa of P. carinii antigen. These results suggest that ganglioside antigens expressed on P. carinii can trigger specific immune responses.

Copyright
COPYRIGHT: © Cambridge University Press 1992
References
Hide All
Colman, P. G., Nayack, R. C., Campbeli, I. L. & Eisenbarth, G. S. (1988). Binding of cytoplasmic islet cell antibodies is blocked by human pancreatic glycolipid. Diabetes 37, 645–52.
Folch, J., Lees, M. & Stanley, G. H. S. (1957). A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226, 497–509.
Formisano, S., Johnson, M. L., Lee, G., Aloj, S. M. & Edelhoc, H. (1979). Critical micelle concentrations of gangliosides. Biochemistry 18, 1119–24.
Gigliotti, F., Ballou, L. R., Hughes, W. T. & Mosley, B. D. (1988). Purification and initial characterization of a ferret Pneumocystis carinii surface antigen. Journal of Infectious Diseases 158, 848–54.
Graves, D. C., McNabb, S. J. N., Ivey, M. H. & Worley, M. A. (1986). Development and characterization of monoclonal antibodies to Pneumocystis carinii. Infection and Immunity 51, 125–33.
Kates, M. (1990). Glycolipids of high plants, algae, yeasts, and fungi. In Glycolipids, Phosphoglycolipids and Sulfoglycolipids, (ed. Kates, M.), pp. 235–308. New York and London: Plenum Press.
Kiguchi, K., Henning-Chubb, B. & Huberman, E. (1990). Glycosphingolypid patterns of peripheral blood lymphocytes, monocytes, and granulocytes are cell specific. Journal of Biochemistry 107, 8–14.
Kovacs, J. A., Halpern, J. L., Swan, J. C., Moss, J., Parrillo, J. E. & Masur, H. (1988). Identification of antigens and antibodies specific for Pneumocystis carinii. Journal of Immunology 140, 2023–31.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–5.
Masala, C., Sorice, M., Di Prima, M. A., Lenti, L., Misasi, R., Mojon, M., Contini, C. & Vullo, V. (1989). Evidence for shared epitopes between cardiolipin and Pneumocystis carinii. Journal of Infectious Diseases 160, 736–7.
Mojon, M. (1976). Serodiagnostic des affection à Pneumocystis carinii. Premiere resultats. Lyon, France: Universitè Claude Bernard.
Pesanti, E. L. (1987). Phospholipid profile of Pneumnocystis carinii and its interaction with alveolar type II epithelial cells. Infection and Immunity 55, 736–41.
Pesanti, E. L. & Shanley, J. D. (1988). Glycoproteins of Pneumocystis carinii: characterization by electrophoresis and microscopy. Journal of Infectious Diseases 158, 1353–9.
Sorice, M., Lenti, L., Misasi, R., Di Prima, M. A., Griggi, T., Cignarella, L., Vullo, V. & Masala, C. (1991). Antiglycosphingolipid antibodies in HIV infection. AIDS 5, 345–6.
Strayer, D. S., Hallman, M. & Merrit, T. A. (1991). Immunogenicity of surfactant. I. Human alveolar surfactant. Clinical Experimental Immunology 83, 35–40.
Sut, A., Sirugue, S., Sixou, S., Lakhdar-Ghazai, F., Tocanne, J. F., Laneelle, G. (1990). Mycobacteria glycolipids as potential pathogenicity effectors: alteration of model and natural membranes. Biochemistry 29, 8498–502.
Tanabe, K., Takasaki, S., Watanabe, J. I., Kobata, A., Egawa, K. & Nakamura, Y. (1989). Glycoproteins composed of major surface immunodeterminants of Pneumocystis carinii. Infection and Immunity 57, 1363–8.
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA 76, 4350–4.
Walzer, P. D., Rutledge, M. E., Yoneda, K., Stahr, B. J. (1979). Pneumocystis carinii: new separation method from lung tissue. Experimental Parasitology 47, 356–68.
Williams, M. A. & McCluer, R. H. (1980). The use of Sep-pac C18 cartridges during the isolation of gangliosides. Journal of Neurochemistry 35, 266–9.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 51 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 12th June 2018. This data will be updated every 24 hours.

Cancel
Confirm
×