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Evidence for the existence of ganglioside molecules on Pneumocystis carinii from human lungs

Published online by Cambridge University Press:  06 April 2009

M. Sorice ,
L. Lenti ,
R. Misasi ,
C. Contini ,
L. Cignarella ,
T. Griggi ,
V. Vullo  and
C. Masala
M. Sorice
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
L. Lenti
Affiliation:
Department of Experimental Medicine, University of Rome, Rome, Italy
R. Misasi
Affiliation:
Department of Experimental Medicine, University of Rome, Rome, Italy
C. Contini
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
L. Cignarella
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
T. Griggi
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
V. Vullo
Affiliation:
Institute of Infectious Disease, University of Rome, Rome, Italy
C. Masala
Affiliation:
Clinics of Tropical and Infectious Disease, University of Rome, Rome, Italy
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Extract

This study was undertaken to assess whether glycolipid antigens (particularly gangliosides) are associated with Pneumocystis carinii obtained from human lungs. Gangliosides were extracted, purified in high performance thin-layer chromatography and stained with resorcinol. Two resorcinol-positive bands, co-migrating with GM1 and GD1a were demonstrated, suggesting the existence of ganglioside molecules on P. carinii. No resorcinol-positive bands were revealed in the pulmonary control tissue. In addition, an antiserum obtained from rabbits immunized with P. carinii antigen reacted with gangliosides GM1, and GD1a, as revealed by a dot immunobinding assay. This reactivity was inhibited by first incubating the antiserum with ganglioside micelles. Furthermore, anti-glycosphingolipid antibodies (aGM1) reacted with the bands of 200 and 55 kDa of P. carinii antigen. These results suggest that ganglioside antigens expressed on P. carinii can trigger specific immune responses.

Keywords

gangliosidesPneumocystis cariniiglycolipids
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 1 - 6
Copyright
Copyright © Cambridge University Press 1992

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