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The first case of microsporidiosis in Paramecium

Published online by Cambridge University Press:  27 April 2020

Yulia Yakovleva ,
Elena Nassonova ,
Natalia Lebedeva ,
Olivia Lanzoni ,
Giulio Petroni ,
Alexey Potekhin  and
Elena Sabaneyeva Open the ORCID record for Elena Sabaneyeva [Opens in a new window]
Yulia Yakovleva
Affiliation:
Department of Cytology and Histology, Saint Petersburg State University, Universitetskaya emb. 7/9, 199034Saint Petersburg, Russian Federation
Elena Nassonova
Affiliation:
Laboratory of Cytology of Unicellular Organisms, Institute of Cytology RAS, Tikhoretsky ave. 4, 194064Saint Petersburg, Russian Federation Department of Invertebrate Zoology, Saint Petersburg State University, Universitetskaya emb. 7/9, 199034Saint Petersburg, Russian Federation
Natalia Lebedeva
Affiliation:
Core Facility Center for Cultivation of Microorganisms, Saint Petersburg State University, Peterhof, Botanicheskaya st. 17, 198504Saint Petersburg, Russian Federation
Olivia Lanzoni
Affiliation:
Department of Biology, University of Pisa, via A Volta 4, 56126Pisa, Italy
Giulio Petroni
Affiliation:
Department of Biology, University of Pisa, via A Volta 4, 56126Pisa, Italy
Alexey Potekhin
Affiliation:
Department of Microbiology, Saint Petersburg State University, 16th line, Vasilyevsky Island, 29, 199178Saint Petersburg, Russian Federation
Elena Sabaneyeva*
Affiliation:
Department of Cytology and Histology, Saint Petersburg State University, Universitetskaya emb. 7/9, 199034Saint Petersburg, Russian Federation
*
Author for correspondence: Elena Sabaneyeva, E-mail: e.sabaneeva@spbu.ru
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Abstract

A new microsporidian species, Globosporidium paramecii gen. nov., sp. nov., from Paramecium primaurelia is described on the basis of morphology, fine structure, and SSU rRNA gene sequence. This is the first case of microsporidiosis in Paramecium reported so far. All observed stages of the life cycle are monokaryotic. The parasites develop in the cytoplasm, at least some part of the population in endoplasmic reticulum and its derivates. Meronts divide by binary fission. Sporogonial plasmodium divides by rosette-like budding. Early sporoblasts demonstrate a well-developed exospore forming blister-like structures. Spores with distinctive spherical shape are dimorphic in size (3.7 ± 0.2 and 1.9 ± 0.2 μm). Both types of spores are characterized by a thin endospore, a short isofilar polar tube making one incomplete coil, a bipartite polaroplast, and a large posterior vacuole. Experimental infection was successful for 5 of 10 tested strains of the Paramecium aurelia species complex. All susceptible strains belong to closely related P. primaurelia and P. pentaurelia species. Phylogenetic analysis placed the new species in the Clade 4 of Microsporidia and revealed its close relationship to Euplotespora binucleata (a microsporidium from the ciliate Euplotes woodruffi), to Helmichia lacustris and Mrazekia macrocyclopis, microsporidia from aquatic invertebrates.

Keywords

CiliateGlobosporidiumhost specificityintracellular parasitismmicrosporidiaParameciumphylogeny
Type
Research Article
Information
Parasitology , Volume 147 , Issue 9 , August 2020 , pp. 957 - 971
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Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press

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