Skip to main content

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 5
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Ditgen, Dana Anandarajah, Emmanuela M. Meissner, Kamila A. Brattig, Norbert Wrenger, Carsten and Liebau, Eva 2014. Harnessing the Helminth Secretome for Therapeutic Immunomodulators. BioMed Research International, Vol. 2014, Issue. , p. 1.
    Hewitson, James P. Harcus, Yvonne Murray, Janice van Agtmaal, Maaike Filbey, Kara J. Grainger, John R. Bridgett, Stephen Blaxter, Mark L. Ashton, Peter D. Ashford, David A. Curwen, Rachel S. Wilson, R. Alan Dowle, Adam A. and Maizels, Rick M. 2011. Proteomic analysis of secretory products from the model gastrointestinal nematode Heligmosomoides polygyrus reveals dominance of Venom Allergen-Like (VAL) proteins. Journal of Proteomics, Vol. 74, Issue. 9, p. 1573.
    Hewitson, James P. Grainger, John R. and Maizels, Rick M. 2009. Helminth immunoregulation: The role of parasite secreted proteins in modulating host immunity. Molecular and Biochemical Parasitology, Vol. 167, Issue. 1, p. 1.
    Cantacessi, C. Campbell, B.E. Visser, A. Geldhof, P. Nolan, M.J. Nisbet, A.J. Matthews, J.B. Loukas, A. Hofmann, A. Otranto, D. Sternberg, P.W. and Gasser, R.B. 2009. A portrait of the “SCP/TAPS” proteins of eukaryotes — Developing a framework for fundamental research and biotechnological outcomes. Biotechnology Advances, Vol. 27, Issue. 4, p. 376.
    Visser, A. Van Zeveren, A.M. Meyvis, Y. Peelaers, I. Van den Broeck, W. Gevaert, K. Vercruysse, J. Claerebout, E. and Geldhof, P. 2008. Gender-enriched transcription of activation associated secreted proteins in Ostertagia ostertagi. International Journal for Parasitology, Vol. 38, Issue. 3-4, p. 455.
    ×
  • Get access
    Add to cart USD35.00
    Check if you have access via personal or institutional login
    Log in Register Recommend to librarian

Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda)

  • H. SAVERWYNS (a1), A. VISSER (a1), A. J. NISBET (a2), I. PEELAERS (a1), K. GEVAERT (a3), J. VERCRUYSSE (a1), E. CLAEREBOUT (a1) and P. GELDHOF (a1)...
Summary

It has been shown that the bovine abomasal parasite, Ostertagia ostertagi, drastically modulates its microenvironment, causing epithelial cell damage, accumulation of inflammatory cells and pH changes in the stomach. The mechanisms used by the parasite to change the abomasal environment are largely unknown, but an important role has been attributed to excretory-secretory (ES) products from the parasite. In this study we have identified proteins representing a novel ES protein family, characterized by the SCP/Tpx-1/Ag5/PR-1/Sc7 protein motif. These proteins were named Oo-AL1 and Oo-AL2 (O. ostertagi ASP-like protein). Both proteins contain a signal peptide and 1 predicted N-glycosylation site. The transcript for Oo-AL1 was present from the L4 stage onwards in both male and female adult worms, whereas the Oo-AL2 transcript was hardly detectable. Western blots of somatic extracts and ES products from different developmental stages of O. ostertagi, probed with anti-Oo-AL1 antibodies, revealed Oo-AL proteins in the ES products of adult worms. An analysis of the nematode genome and EST databases indicated that these novel ES proteins are unique to O. ostertagi and its relative, Teladorsagia circumcincta, suggesting a key function in these abomasal parasites.

Copyright
COPYRIGHT: © Cambridge University Press 2007
Corresponding author
*Corresponding author: Laboratory for Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium. Tel: +32 92647387. Fax: +32 92647496. E-mail: peter.geldhof@UGent.be
References
Hide All
Basse, C. W. (2005). Dissecting defense-related and developmental transcriptional responses of maize during Ustilago maydis infection and subsequent tumor formation. Plant Physiology 138, 1774–1784. doi: 10.1104/pp.105.061200.
Bethony, J., Loukas, A., Smout, M., Brooker, S., Mendez, S., Plieskatt, J., Goud, G., Bottazzi, M. E., Zhan, B., Wang, Y., Williamson, A., Lustigman, S., Correa-Oliveira, R., Xiao, S., Hotez, P. J. (2005). Antibodies against a secreted protein from hookworm larvae reduce the intensity of hookworm infection in humans and vaccinated laboratory animals. FASEB Journal 12, 1743–1745. doi: 10.1096/fj.05-3936fje.
Claerebout, E. and Vercruysse, J. (2000). The immune response and the evolution of acquired immunity against gastrointestinal nematodes in cattle. Parasitology 120, S25–S40.
De Maere, V., Vercauteren, I., Saverwyns, H., Claerebout, E., Berx, G. and Vercruysse, J. (2002). Identification of potential protective antigens of Ostertagia ostertagi with local antibody probes. Parasitology 125, 383–391. doi: 10.1016/j.molbiopara.2005.01.018.
Dixon, D. C., Cutt, J. R. and Klessig, D. F. (1991). Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts. The EMBO Journal 10, 1317–1324.
Durrant, W. E. and Dong, X. (2004). Systemic acquired resistance. Annual Review of Phytopathology 42, 185–209. doi: 10.1146/annurev.phyto.42.040803.140421.
Frangioni, J. V. and Neel, B. G. (1993). Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Analytical Biochemistry 210, 179–187. doi: 10.1006/abio.1993.1170.
Geldhof, P., Claerebout, E., Knox, D. P., Agneessens, J. and Vercruysse, J. (2000). Proteinases released in vitro by the parasitic stages of the bovine abomasal nematode Ostertagia ostertagi. Parasitology 121, 639–647. doi: 10.1017/S0031182000006806.
Geldhof, P., Claerebout, E., Knox, D., Vercauteren, I., Looszova, A. and Vercruysse, J. (2002). Vaccination of calves against Ostertagia ostertagi with cysteine proteinase enriched protein fractions. Parasite Immunology 24, 263–270. doi: 10.1046/j.1365-3024.2002.00461.x.
Geldhof, P., Vercauteren, I., Gevaert, K., Staes, A., Knox, D. P., Vandekerckhove, J., Vercruysse, J. and Claerebout, E. (2003). Activation-associated secreted proteins are the most abundant antigens in a host protective fraction from Ostertagia ostertagi. Molecular and Biochemical Parasitology 128, 111–114. doi: 10.1016/S0166-6851(03)00044-6.
Geldhof, P., Vercauteren, I., Vercruysse, J., Knox, D. P., van den Broeck, W. and Claerebout, E. (2004). Validation of the protective Ostertagia ostertagi ES-thiol antigens with different adjuvantia. Parasite Immunology 26, 37–43. doi: 10.1111/j.0141-9838.2004.00681.x.
Geldhof, P., Whitton, C., Gregory, W. F., Blaxter, M. and Knox, D. P. (2005). Characterisation of the two most abundant genes in the Haemonchus contortus expressed sequence tag dataset. International Journal for Parasitology 35, 513–522. doi: 10.1016/j.ijpara.2005.02.009.
Hawdon, J. M., Jonest, B. F., Hoffman, D. R. and Hotez, P. J. (1996). Cloning and characterization of Ancylostoma-secreted protein. A novel protein associated with the transition to parasitism by infective hookworm larvae. The Journal of Biological Chemistry 271, 6672–6678.
Hawdon, J. M., Narasimhan, S. and Hotez, P. J. (1999). Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum. Molecular and Biochemical Parasitology 30, 149–165. doi: 10.1016/S0166-6851(99)00011-0.
Kasahara, M., Gutknecht, J., Brew, K., Spurr, N. and Goodfellow, P. N. (1989). Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene. Genomics 5, 527–534.
Kent, W. J. and Zahler, A. M. (2000). Conservation, regulation, synteny, and introns in a large-scale C. briggsae-C. elegans genomic alignment. Genome Research 10, 1115–1125.
Klesius, P. H. (1993). Regulation of immunity to Ostertagia ostertagi. Veterinary Parasitology 46, 63–79.
Knox, D. P. (2000). Development of vaccines against gastrointestinal nematodes. Parasitology 20, S43–S61.
Kumar, S., Tamura, K. and Nei, M. (2004). MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Briefings in Bioinformatics 5, 150–163.
Lu, G., Villalba, M., Coscia, M. R., Hoffman, D. R. and King, T. P. (1993). Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets. The Journal of Immunology 150, 2823–2830.
McKellar, Q. A. (1993). Interactions of Ostertagia species with their bovine and ovine hosts. International Journal for Parasitology 23, 451–462.
Moser, J. M., Freitas, T., Arasu, P. and Gibson, G. (2005). Gene expression profiles associated with the transition to parasitism in Ancylostoma caninum larvae. Molecular and Biochemical Parasitology 143, 39–48. doi: 10.1016/j.molbiopara.2005.04.012.
Murray, J., Gregory, W. F., Gomez-Escobar, N., Atmadja, A. K. and Maizels, R. M. (2001). Expression and immune recognition of Brugia malayi VAL-1, a homologue of vespid venom allergens and Ancylostoma secreted proteins. Molecular and Biochemical Parasitology 118, 89–96. doi: 10.1016/S0166-6851(01)00374-7.
Nagasaki, H., Arita, M., Nishizawa, T., Suwa, M. and Gotoh, O. (2005). Species-specific variation of alternative splicing and transcriptional initiation in six eukaryotes. Gene 30, 53–62. doi: 10.1016/j.gene.2005.07.027.
Parkinson, J., Mitreva, M., Whitton, C., Thomson, M., Daub, J., Martin, J., Schmid, R., Hall, N., Barrell, B., Waterston, R. H., McCarter, J. P. and Blaxter, M. L. (2004). A transcriptomic analysis of the phylum Nematoda. Nature Genetics 36, 1259–1267. doi: 10.1038/ng1472.
Redmond, D. L., Smith, S. K., Halliday, A., Smith, W. D., Jackson, F., Knox, D. P. and Matthews, J. B. (2006). An immunogenic cathepsin F secreted by the parasitic stages of Teladorsagia circumcincta. International Journal for Parasitology 36, 277–286.
Schallig, H. D. F. H., Van Leeuwen, M. A. W., Cornelissen, A. W. C. A. (1997). Protective immunity induced by vaccination with two Haemonchus contortus excretory secretory proteins in sheep. Parasite Immunology 19, 447–453. doi: 10.1046/j.1365-3024.1997.d01-148.x.
Sheth, N., Roca, X., Hastings, M. L., Roeder, T., Krainer, A. R. and Sachidanandam, R. (2006). Comprehensive splice-site analysis using comparative genomics. Nucleic Acids Research 34, 3955–3967. doi: 10.1093/nar/glk556.
Simpson, H. V. (2000). Pathophysiology of abomasal parasitism: is the host or parasite responsible? The Veterinary Journal 160, 177–191. doi: 10.1053/tvjl.2000.0491.
Simpson, H. V., Simpson, B. H., Simcock, D. C., Reynolds, G. W. and Pomroy, W. E. (1999). Abomasal secretion in sheep receiving adult Ostertagia circumcinta that are prevented from contact with the mucosa. New Zealand Veterinary Journal 47, 20–24.
Van Loon, L. C. and Van Strien, E. A. (1999). The families of pathogenesis-related proteins, their activities, and comparative analysis of PR-1 type proteins. Physiological and Molecular Plant Pathology 55, 85–97.
Van Zeveren, A. M., Visser, A., Hoorens, P. R., Vercruysse, J., Claerebout, E. and Geldhof, P. (2007). Evaluation of reference genes for quantitative real-time PCR in Ostertagia ostertagi by the coefficient of variation and geNorm approach. Molecular and Biochemical Parasitology 153, 224–227. doi: 10.1016/j.molbiopara.2007.03.005.
Vercauteren, I., Geldhof, P., Peelaers, I., Claerebout, E., Berx, G. and Vercruysse, J. (2003). Identification of excretory-secretory products of larval and adult Ostertagia ostertagi by immunoscreening of cDNA libraries. Molecular and Biochemical Parasitology 126, 201–208. doi: 10.1016/S0166-6851(02)00274-8.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Keywords:

Metrics

Full text views

Total number of HTML views: 1
Total number of PDF views: 16 *
Loading metrics...

Abstract views

Total abstract views: 74 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 12th June 2018. This data will be updated every 24 hours.

Cancel
Confirm
×