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Immunological characterization of a chimeric form of Schistosoma mansoni aquaporin in the murine model

Published online by Cambridge University Press:  01 May 2014

BARBARA CASTRO PIMENTEL FIGUEIREDO ,
NATAN RAIMUNDO GONÇALVES DE ASSIS ,
SUELLEN BATISTONI DE MORAIS ,
VICENTE PAULO MARTINS Open the ORCID record for VICENTE PAULO MARTINS [Opens in a new window] ,
NATASHA DELAQUA RICCI ,
RODRIGO MARQUES BICALHO ,
CARINA DA SILVA PINHEIRO  and
SERGIO COSTA OLIVEIRA
BARBARA CASTRO PIMENTEL FIGUEIREDO
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
NATAN RAIMUNDO GONÇALVES DE ASSIS
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
SUELLEN BATISTONI DE MORAIS
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
VICENTE PAULO MARTINS
Affiliation:
Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil Departamento de Biologia Celular do Instituto de Ciências Biológicas, Universidade de Brasília, 70910-900 Brasília, DF, Brazil
NATASHA DELAQUA RICCI
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
RODRIGO MARQUES BICALHO
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
CARINA DA SILVA PINHEIRO
Affiliation:
Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil Departamento de Biointeração do Instituto de Ciências da Saúde, Universidade Federal da Bahia, 40110-100, Salvador, BA, Brazil
SERGIO COSTA OLIVEIRA*
Affiliation:
Departamento de Bioquímica e Imunologia do Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31270-901 Belo Horizonte, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, 31270-901, MG, Brazil
*
*Corresponding author: Av. Antônio Carlos, 6627 – Pampulha – Caixa Postal 486, Belo Horizonte, MG, Brazil31270-901. E-mail: scozeus1@gmail.com
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Summary

Aquaporin (SmAQP) is the most abundant transmembrane protein in the tegument of Schistosoma mansoni. This protein is expressed in all developmental stages and seems to be essential in parasite survival since it plays a crucial role in osmoregulation, nutrient transport and drug uptake. In this study, we utilized the murine model to evaluate whether this protein was able to induce protection against challenge infection with S. mansoni cercariae. A chimeric (c) SmAQP was formulated with Freund's adjuvant for vaccination trial and evaluation of the host's immune response was performed. Our results demonstrated that immunization with cSmAQP induced the production of high levels of specific anti-cSmAQP IgG antibodies and a Th1/Th17 type of immune response characterized by IFN-γ, TNF-α and IL-17 cytokines. However, vaccination of mice with cSmAQP failed to reduce S. mansoni worm burden and liver pathology. Finally, we were unable to detect humoral immune response anti-cSmAQP in the sera of S. mansoni-infected human patients. Our results lead us to believe that SmAQP, as formulated in this study, may not be a good target in the search for an anti-schistosomiasis vaccine.

Keywords

Schistosoma mansonitegumentaquaporinvaccineTh1/Th17 response
Type
Research Article
Information
Parasitology , Volume 141 , Issue 10 , September 2014 , pp. 1277 - 1288
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Copyright
Copyright © Cambridge University Press 2014 

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