Skip to main content
    • Aa
    • Aa
  • Get access
    Check if you have access via personal or institutional login
  • Cited by 3
  • Cited by
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Hempelmann, Ernst and Marques, Helder M. 1994. Analysis of malaria pigment from Plasmodium falciparum. Journal of Pharmacological and Toxicological Methods, Vol. 32, Issue. 1, p. 25.

    Kaushal, Deep C. Watts, Ranjana Haider, Seema Singh, Neeloo Kaushal, Nuzhat A. and Dutta, Guru P. 1988. Antibodies to Lactate Dehydrogenase ofPlasmodium Knowlesiare Specific toPlasmodiumSpecies. Immunological Investigations, Vol. 17, Issue. 6-7, p. 507.

    Ling, I. T. Cooksley, S. Bates, P. A. Hempelmann, E. and Wilson, R. J. M. 1986. Antibodies to the glutamate dehydrogenase of Plasmodium falciparum. Parasitology, Vol. 92, Issue. 02, p. 313.


Immunoprecipitation of malarial acid endopeptidase

  • E. Hempelmann (a1), B. Putfarken (a2), K. Rangachari (a3) and R. J. M. Wilson (a4)
  • DOI:
  • Published online: 01 April 2009

Electrophoresis of extracts of schizonts of Plasmodium knowlesi in non-dissociating polyacrylamide gels, separates several bands of acid endopeptidase activity. A polyclonal antiserum, produced by immunization with purified merozoites, failed to distinguish between different bands of the parasite enzyme, indicating that they are serologically related. Apart from the loss of one minor peak, extraction in Triton X-100 did not reduce the enzyme's electrophoretic heterogeneity. The antiserum did not react with red cell acid proteases.

Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

H. S. Banyal , G. C. Misra , C. M. Gupta & G. P. Dutta (1981). Involvement of malarial proteases in the interaction between the parasite and host erythrocyte in Plasmodium knowlesi infections. Journal of Parasitology 67, 623–6.

P. H. David , T. J. Hadley , M. Alkawa & C. H. Miller (1984). Processing of a major parasite surface glycoprotein during the ultimate stages of differentiation in Plasmodium knowlesi. Molecular and Biochemical Parasitology 11, 267–82.

T. G. Geary , E. J. Delaney , I. M. KLotz & J. B. Jensen (1983). Inhibition of the growth of Plasmodium falciparum in vitro by covalent modification of haemoglobin. Molecular and Biochemical Parasitology 9, 5972.

F. N. Gyang , B. Poole & W. Trager (1982). Peptidases from Plasmodium falciparum cultured in vitro. Molecular and Biochemical Parasitology 5, 203–73.

C. A. Homewood , D. C. Warhurst , W. Peters & V. C. Baggaley (1972). Lysosomes, pH and the antimalarial action of chloroquine. Nature, London 235, 50–2.

M. R. Levy , W. A. Siddiqui & S. C. Chou (1974). Acid protease activity in Plasmodium falciparum and P. knowlesi and ghosts of their respective host red cells. Nature, London 247, 546–9.

R. J. Mayer & J. H. Walker (1980). Immunochemical Methods in the Biological Sciences: Enzymes and Proteins. New York: Academic Press.

S. Pontremoli , B. Sparatoras , E. Melloni , F. Salamino , M. Michetti , A. Morelli , H. Benetti & A. De Flora (1980). Differences and similarities among three acidic endopeptidases associated with human erythrocyte membranes. Biochimica et Biophysica Acta 630, 313–22.

J. Schrevel , F. Bernard , C. Maintier , R. Mayer & M. Monsigny (1984). Detection and characterization of a selective endopeptidase from Plasmodium berghei by using fluorogenic peptidyl substrates. Biochemical and Biophysical Research Communications 124, 703–10.

I. W. Sherman & L. Tsnigoshi (1983). Purification of Plasmodium lophurae cathepsin D and its effects on erythrocyte membrane proteins. Molecular and Biochemical Parasitiology 8, 207–26.

C. Slomianny , P. Charet & G. Prensier (1983). Ultrastructural localization of enzymes involved in the feeding processes in Plasmodium chabaudi and Babesia hylomysi. Journal of Protozoology 30, 376–82.

D. L. Vander Jagt , B. R. Baack & L. A. Hunsaker (1984). Purification and characterization of an aminopeptidase from Plasmodium falciparum. Molecular and Biochemical Parasitology 10, 4554.

K. Yamamoto , N. Katsuda , M. Himeno & K. Kato (1979). Cathepsin D of rat spleen. Affinity purification and properties of two types of Cathepsin D. European Journal of Biochemistry 95, 459–67.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *