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Increased cell proliferation of mouse fibroblast NIH-3T3 in vitro induced by excretory/secretory product(s) from Opisthorchis viverrini

  • C. THUWAJIT (a1) (a2), P. THUWAJIT (a1) (a2), S. KAEWKES (a3) (a2), B. SRIPA (a4) (a2), K. UCHIDA (a5), M. MIWA (a5) and S. WONGKHAM (a1) (a2)...
Abstract

Infection by Opisthorchis viverrini is a strong risk factor for cholangiocarcinoma. However, the mechanism by which the parasite is involved in carcinogenesis is not clear. In addition to the direct damage of the bile duct epithelium via direct contact with O. viverrini, the excretory/secretory (ES) product(s) released from the parasites may play important roles in this process. We therefore investigated the responses of a fibroblast cell line, NIH-3T3, to ES product(s) released from O. viverrini by using a non-contact co-culture technique. In this culture system, the parasites in the upper chamber had no direct contact with the NIH-3T3 cells in the lower chamber of the culture plate. The results indicated a marked increase in NIH-3T3 cell proliferation in the non-contact co-culture condition with either 0% or 10% calf serum in the medium compared with that without parasites. ES product(s) increased cell proliferation by stimulating the expression of phosphorylated retinoblastoma (pRB) and cyclin D1, the key proteins in driving cells through the G1/S transition point of the cell cycle. This led to the induction of cells going into the S-phase of the cell cycle. ES product(s) also changed the morphology of NIH-3T3 cells to a refractive and narrow shape, which allowed the cells to proliferate in the limited culture area. For the first time, we have been able to demonstrate increased cell proliferation induced by the ES product(s) from O. viverrini; this finding may clarify how O. viverrini ES product(s) affect human bile duct epithelium during cholangiocarcinogenesis.

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Corresponding author
Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. Tel: +66-43-348386. Fax: +66-43-348386. E-mail: tchani@kku.ac.th
References
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REFERENCES

BHAMARAPRAVATI, N., THAMMAVIT, W. & VAJRASTHIRA, S. (1978). Liver changes in hamsters infected with a liver fluke of man, Opisthorchis viverrini. American Journal of Tropical Medicine and Hygiene 27, 787794.
DANESH, F. R., YE, M., SALMI, S., LAPOINTE, M. & BATLLE, D. (1999). Temporal profile of serum-induced S-phase entry and retinoblastoma protein phosphorylation in human skin fibroblasts. Kidney International 56, 12821285.
DISALVO, C. V., ZHANG, D. & JACOBBERGER, J. W. (1995). Regulation of NIH-3T3 cell G1 phase transit by serum during exponential growth. Cell Proliferation 28, 511524.
GILLE, H. & DOWNWARD, J. (1999). Multiple ras effector pathways contribute to G(1) cell cycle progression. Journal of Biological Chemistry 274, 2203322040.
GROSSI, M., LA ROCCA, S. A., PIERLUIGI, G., VANNUCCHI, S., RUARO, E. M., SCHNEIDER, C. & TATO, F. (1998). Role of Gas1 down-regulation in mitogenic stimulation of quiescent NIH3T3 cells by v-Src. Oncogene 17, 16291638.
INTERNATIONAL AGENCY FOR RESEARCH ON CANCER (1994). Infection with the liver flukes (Opisthorchis viverrini, Opisthorchis felineus and Clonorchis sinensis). IARC Monograp on Evaluation of Carcinogenic Risks of Human 61, 121175.
IYER, V. R., EISEN, M. B., ROSS, D. T., SCHULER, G., MOORE, T., LEE, J. C., TRENT, J. M., STAUDT, L. M., HUDSON, J. Jr., BOGUSKI, M. S., LASHKARI, D., SHALON, D., BOTSTEIN, D. & BROWN, P. O. (1999). The transcriptional program in the response of human fibroblasts to serum. Science 283, 8387.
JONGSUKSUNTIGUL, P. & IMSOMBOON, T. (1998). Epidemiology of opisthorchiasis and national control program in Thailand. Southeast Asian Journal of Tropical Medicine and Public Health 29, 327332.
KERKHOFF, E. & RAPP, U. R. (1997). Induction of cell proliferation in quiescent NIH 3T3 cells by oncogenic c-Raf-1. Molecular and Cellular Biology 17, 25762586.
KIERSZENBAUM, F., CUNA, W. R., BELTZ, L. A. & SZTEIN, M. B. (1990). Trypanosomal immunosuppressive factor: a secretion product(s) of Trypanosoma cruzi that inhibits proliferation and IL-2 receptor expression by activated human peripheral blood mononuclear cells. Journal of Immunology 144, 40004004.
MATSUSHIME, H., QUELLE, D. E., SHURTLEFF, S. A., SHIBUYA, M., SHERR, C. J. & KATO, J. Y. (1994). D-type cyclin-dependent kinase activity in mammalian cells. Molecular and Cellular Biology 14, 20662076.
MIGASENA, P., REAUNSUWAN, W. & CHANGBUMRUNG, S. (1980). Nitrates and nitrites in local Thai preserved protein foods. Journal of Medical Association of Thailand 63, 500505.
MITACEK, E. J., BRUNNEMANN, K. D., SUTTAJIT, M., MARTIN, N., LIMSILA, T., OHSHIMA, H. & CAPLAN, L. S. (1999). Exposure to N-nitroso compounds in a population of high liver cancer regions in Thailand: volatile nitrosamine (VNA) levels in Thai food. Food and Chemical Toxicology 37, 297305.
NORBURY, C. & NURSE, P. (1992). Animal cell cycles and their control. Annual Review of Biochemistry 61, 441470.
PAIROJKUL, C., SHIRAI, T., HIROHASHI, S., THAMAVIT, W., BHUDHISAWAT, W., UTTARAVICIEN, T., ITOH, M. & ITO, N. (1991). Multistage carcinogenesis of liver-fluke-associated cholangiocarcinoma in Thailand. Princess Takamatsu Symposium 22, 7786.
PARKIN, D. M., SRIVATANAKUL, P., KHLAT, M., CHENVIDHYA, D., CHOTIWAN, P., INSIRIPONG, S., L'ABBE, K. A. & WILD, C. P. (1991). Liver cancer in Thailand. I. A case-control study of cholangiocarcinoma. International Journal of Cancer 48, 323328.
PATTANAPANYASAT, K., FLAVELL, D. J. & VONGSANGNAK, V. (1983). A method for collecting metacercariae of Opisthorchis viverrini. Siriraj Hospital Gazette 35, 143146.
PINLAOR, S., YONGVANIT, P., HIRAKU, Y., MA, N., SEMBA, R., OIKAWA, S., MURATA, M., SRIPA, B., SITHITHAWORN, P. & KAWANISHI, S. (2003). 8-Nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini. Biochemical and Biophysical Research Communications 309, 567571.
PLANAS-SILVA, M. D. & WEINBERG, R. A. (1997). The restriction point and control of cell proliferation. Current Opinion in Cell Biology 9, 768772.
RIGANTI, M., PUNGPAK, S., PUNPOOWONG, B., BUNNAG, D. & HARINASUTA, T. (1989). Human pathology of Opisthorchis viverrini infection: a comparison of adults and children. Southeast Asian Journal of Tropical Medicine and Public Health 20, 95100.
SHERR, C. J. & ROBERTS, J. M. (1995). Inhibitors of mammalian G1 cyclin-dependent kinases. Genes and Development 9, 11491163.
SHERR, C. J. (1996). Cancer cell cycles. Science 274, 16721677.
SPENCER, J. A. & MISRA, R. P. (1999). Expression of the SRF gene occurs through a Ras/Sp/SRF-mediated-mechanism in response to serum growth signals. Oncogene 18, 73197327.
TAYLOR, S. J. & SHALLOWAY, D. (1996). Cell cycle-dependent activation of Ras. Current Biology 6, 16211627.
VATANASAPT, V., SRIPA, B., SITHITHAWORN, P. & MAIRIANG, P. (1999). Liver flukes and liver cancer. Cancer Survey 33, 313343.
WATANAPA, P. & WATANAPA, W. B. (2002). Liver fluke-associated cholangiocarcinoma. British Journal of Surgery 89, 962970.
WATANAPA, P. & WILLIAMSON, R. C. (1993). Experimental pancreatic hyperplasia and neoplasia: effects of dietary and surgical manipulation. British Journal of Cancer 67, 877884.
WILLIAMSON, R. C. N. & RAINEY, J. B. (1984). The relationship between intestinal hyperplasia and carcinogenesis. Scandinavian Journal of Gastroenterology 19 (Suppl.), 5776.
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Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
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