Skip to main content
×
×
Home

The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms

  • S. R. Smithers (a1) and R. J. Terry (a1)
Extract

At present many laboratories throughout the world are studying the chemotherapy and immunology of Schistosoma mansoni in laboratory hosts. Many workers judge the success or failure of their attempts to cure or immunize these hosts from the ratio of the number of living adult worms recovered to the number of infecting cercariae. This ratio is affected, however, not only by the efficacy of any treatment, but also by the methods used to infect the animals and to recover the worms. If these methods result in widely varying worm recoveries amongst the animals in any experimental group, then small but significant effects of treatment might well be missed. Alternatively, such large experimental groups must be used that the work becomes tedious to perform and depends upon the availability of a great deal of technical assistance. This paper describes techniques which are rapid and do not require great skill in their performance. More important, in our hands they have given very consistent results. In this respect, particularly, we believe that these techniques have advantages over others which are currently practised.

The techniques described here are those which were used in other investigations reported in this journal (Smithers & Terry, 1965a, b).

The strain of S. mansoni used throughout this work was isolated in Puerto Rico and was obtained through the courtesy of Dr W. B. DeWitt of the National Institutes of Health. The parasite is maintained in an albino strain of Australorbis glabratus (Newton, 1955). Snails are exposed individually to ten miracidia overnight at 27 °C.

Copyright
References
Hide All
Bruce, H. M. & Parkes, A. S. (1949). Feeding and breeding of laboratory animals. IX. A complete cubed diet for mice and rats. J. Hyg., Camb., 47, 202–8.
Newton, W. L. (1955). The establishment of a strain of Australorbis glabratus which combines albinism and high susceptibility to infection with Sohistosoma mansoni. J. Parasit. 41, 526–8.
Radke, M. G., Berrios-Duran, L. A. & Moran, K. (1961). A perfusion procedure (Perf-o-suction) for recovery of schistosome worms. J. Parasit. 47, 366–8.
Smithers, S. R. & Terry, R. J. (1965 a). Naturally acquired resistance to experimental infections of Schistosoma mansoni in the rhesus monkey (Macaca mulatto). Parasitology, 55, 701.
Smithers, S. R. & Terry, R. J. (1965 b). Acquired immunity to experimental infections of Schistosoma mansoni in the albino rat. Parasitology, 55, 711.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed