Skip to main content

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 5
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Chapman, H. D. 1997. Biochemical, genetic and applied aspects of drug resistance inEimeriaparasites of the fowl. Avian Pathology, Vol. 26, Issue. 2, p. 221.
    Coombs, Graham H. Denton, Helen Brown, Samantha M.A. and Thong, Kam-Wah 1997. Advances in Parasitology Volume 39. Vol. 39, Issue. , p. 141.
    Profous-Juchelka, Helen Liberator, Paul and Turner, Mervyn 1988. Identification and characterization of cDNA clones encoding antigens of Eimeria tenella. Molecular and Biochemical Parasitology, Vol. 30, Issue. 3, p. 233.
    Pasternak, Jack Winkfein, Robert J. and Fernando, M.Agnes 1981. Ribosomal and translatable messenger RNA of Eimeria tenella. Molecular and Biochemical Parasitology, Vol. 3, Issue. 3, p. 133.
    James, S. 1980. Isolation of second-generation schizonts of avian coccidia and their use in biochemical investigations. Parasitology, Vol. 80, Issue. 02, p. 301.
    ×
  • Get access
    Add to cart USD35.00
    Check if you have access via personal or institutional login
    Log in Register Recommend to librarian

Isolation of second-generation schizonts of avian coccidia and their use in biochemical investigations

  • S. James (a1)
Summary

A technique is described for the isolation of a pure suspension of second-generation schizonts of Eimeria necatrix and E. tenella. Most of the schizonts appeared viable as judged by their ability to exclude Trypan blue and the use of this criterion is examined. They were able to metabolize radio-isotope labelled glucose and accumulate thiamine against a concentration gradient. A formula allowing detailed consideration of the latter process is introduced. Crude extracts of these schizonts exhibited serine hydroxymethyl transferase activity which is significantly different from that of the host tissue in terms of optimal pH (8·3 for the parasite and 7·8 for the avian intestinal cells) and Michaelis constant (approximately 2·6 × 10−3m and 0·6 × 10−3m respectively). Differences in response to potential inhibitors are also shown.

Copyright
COPYRIGHT: © Cambridge University Press 1980
References
Hide All
Botsford, J. L. Jr & Parks, L. W. (1969). Serine transhydroxymethylase in methionine biosynthesis in Saccharomyces cerevisiae. Journal of Bacteriology 96, 1176–83.
Brazil, R. P. (1978). Isolation of the interacellular stages of Leishmania mexicana amazonerisis using cellulose column. Annals of Tropical Medicine and Parasitology 72, 579–84.
Broderick, R. S., Candland, K. L., North, J. A. & Mangum, J. H. (1972). The isolation of serine transhydroxymethylase from bovine brain. Archives of Biochemistry and Biophysics 148, 196–8.
Coles, A. M., Swoboda, B. E. & Ryley, J. F. (1979). Thymidylate synthetase as a chemotherapeutic target in the treatment of avian coccidiosis. Parasitology 79, li.
Doran, D. J. (1973). Cultivation of coccidia in avian embryos and cell culture. In The Coccidia (ed. Hammond, D. M. and Long, P. L.), pp. 183–252, Baltimore: University Park Press.
Elsner, Y. Y., Roberts, W. L., Shigematsu, A. & Hammond, D. M. (1974). Isolation of schizonts and merozoites of Eimeria callospermophili from cultured bovine intestinal cells. Journal of Parasitology 60, 531–2.
Fernando, M. A. & Pasternak, J. (1977). Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix. Parasitology 74, 19–26.
Gutteridge, W. E. & Coombs, G. H. (1977). Biochemistry of Parasitic Protozoa, pp. 69–88. London: Macmillan Press.
Gutteridge, W. E., Cover, B. & Gaborak, M. (1978). Isolation of blood and intracellular forms of Trypanosoma cruzi from rats and other rodents and preliminary studies of the metabolism. Parasitology 76, 159–76.
Harish-Kumar, R. M., North, J. A., Mangum, J. H. & Rao, N. A. (1976). Co-operative interactions of tetrahydrofolate with purified pig kidney serine transhydroxymethylase. Proceedings of the National Academy of Sciences, USA 73, 1950–.
Huennekens, F. M. & Osborne, M. J. (1959). Folio acid co-enzymes and one-carbon metabolism. Advances in Enzymology 21, 369–446.
Kimmich, G. A. (1970). Preparation and properties of mucosal cells isolated from the small intestine of the chicken. Biochemistry 9, 3659–68.
Kimmich, G. A. (1975). Methods in Membrane Biology 5 (ed. Korn, Edward D.) pp. 51–115, London: Plenum Press.
James, S. (1980). Thiamine uptake in isolated schizonts of Eimeria tenella and the inhibitory effects of amprolium. Parasitology 80, 313–22.
Peterson, G. L. (1977). A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83, 346–56.
Platzer, E. G. (1968). Metabolism of tetrahydrofolate in Plasmodium lophurae and duckling erythrocytes. Transactions of the New York Academy of Science 34, 200–8.
Platzer, E. G. & Campuzano, H. C. (1976). The serine hydroxymethyl transferase of Plasmodium lophurae. Journal of Protozology 23, 282–6.
Rognstad, R. & Katz, J. (1969). Effect of 2,4-dinitrophenol on adipose tissue metabolism. Biochemical Journal 111, 431–44.
Rose, M. E. (1973). Immunity. In The Coccidia (ed. Hammond, D. M. and Long, P. L.), pp. 295–341. Baltimore: University Park Press.
Rose, M. E. & Hesketh, P. (1976). Immunity to coccidiosis: stages of the life-cycle of Eimeria maxima which induce and are affected by the response of the host. Parasitology 73, 25–37.
Ryley, J. F. (1973). Cytochemistry, physiology and biochemistry. In The Coccidia (ed. Hammond, D. M. and Long, D. L.), pp. 145–181. Baltimore: University Park Press.
Schirch, L. V. & Diller, A. (1971). Serine transhydroxymethylase. Journal of Biological Chemistry 246, 3961–6.
Scrimgeour, K. G. & Huennekens, F. M. (1968). Serine hydroxymethylase. Methods in Enzymology 6, 838–43.
Smith, C. C., McCormick, G. J. & Canfield, C. J. (1976). Plasmodium knowlesi: in vitro biosynthesis of methionine. Experimental Parasitology 40, 432–7.
Taylor, R. T. & Weissbach, H. (1965). Radioactive assay for serine transhydroxymethylase. Analytical Biochemistry 13, 80–4.
Wang, C. C. (1976). Inhibition of respiration of Eimeria tenella by quinolone coccidiostats. Biochemical Pharmacology 25, 343–9.
Wang, C. C. (1978 a). Multiple leucine aminopeptidases in Eimeria tenella and their changes during sporulation. Comparative Biochemistry and Physiology 61B 307–13.
Wang, C. C. (1978 b). Avian Coccidiosis. Poultry Science Symposium Number Thirteen (ed. Long, P. L., Boorman, K. N. and Freeman, B. M.) pp. 135–184. Edinburgh: Longmans.
Wang, C. C., Stotish, R. L. & Poe, M. (1975). Dihydrofolate reductase from Eimeria tenella: rationalisation of action of pyrimethamine. Journal of Protozoology 22, 564–8.
Wilson, P. A. G. & Fairbairn, D. (1961). Biochemistry of sporulation in oocysts of Eimeria acervulina. Journal of Protozoology 8, 410–16.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 2 *
Loading metrics...

Abstract views

Total abstract views: 46 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 12th June 2018. This data will be updated every 24 hours.

Cancel
Confirm
×