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Levels of circulating anti-muscarinic and anti-adrenergic antibodies and their effect on cardiac arrhythmias and dysautonomia in murine models of Chagas disease

Published online by Cambridge University Press:  05 August 2014

ANISSA DALIRY ,
ISABELA RESENDE PEREIRA ,
PEDRO PAULO PEREIRA-JUNIOR ,
ISALIRA PEROBA RAMOS ,
GLAUCIA VILAR-PEREIRA ,
RAQUEL RANGEL SILVARES ,
JOSELI LANNES-VIEIRA  and
ANTÔNIO CARLOS CAMPOS DE CARVALHO
ANISSA DALIRY
Affiliation:
Laboratório de Investigação Cardiovascular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil Laboratório de Cardiologia Celular e Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil
ISABELA RESENDE PEREIRA
Affiliation:
Laboratório de Biologia das Interações, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
PEDRO PAULO PEREIRA-JUNIOR
Affiliation:
Laboratório de Eletrofisiologia Cardíaca, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil
ISALIRA PEROBA RAMOS
Affiliation:
Laboratório de Cardiologia Celular e Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil Departamento de Radiologia, Hospital Universitário Clementino Fraga Filho, Rio de Janeiro, Brazil
GLAUCIA VILAR-PEREIRA
Affiliation:
Laboratório de Biologia das Interações, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
RAQUEL RANGEL SILVARES
Affiliation:
Laboratório de Investigação Cardiovascular, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
JOSELI LANNES-VIEIRA
Affiliation:
Laboratório de Biologia das Interações, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, RJ, Brazil
ANTÔNIO CARLOS CAMPOS DE CARVALHO*
Affiliation:
Laboratório de Cardiologia Celular e Molecular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, UFRJ, Rio de Janeiro, Brazil
*
*Corresponding author: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Av. Carlos Chagas Filho, 373, Bloco G, sala G2-045, Cidade Universitária, Rio de Janeiro, RJ, CEP 21941-902, Brazil. E-mail: acarlos@biof.ufrj.br
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Summary

Antibodies (Ab) recognizing G-protein coupled receptors, such as β1 and β2 adrenergic (anti-β1-AR and anti-β2-AR, respectively) and muscarinic cholinergic receptors (anti-M2-CR) may contribute to cardiac damage, however their role in chronic chagasic cardiomyopathy is still controversial. We describe that Trypanosoma cruzi-infected C3H/He mice show increased P and QRS wave duration, and PR and QTc intervals, while the most significant ECG alterations in C57BL/6 are prolonged P wave and PR interval. Echocardiogram analyses show right ventricle dilation in infected animals of both mouse lineages. Analyses of heart rate variability (HRV) in chronically infected C3H/He mice show no alteration of the evaluated parameters, while C57BL/6 infected mice display significantly lower values of HRV components, suggesting autonomic dysfunction. The time-course analysis of anti-β1-AR, anti-β2-AR and anti-M2-CR Ab titres in C3H/He infected mice indicate that anti-β1-AR Ab are detected only in the chronic phase, while anti-β2-AR and anti-M2-CR are observed in the acute phase, diminish at 60 dpi and increase again in the chronic phase. Chronically infected C57BL/6 mice presented a significant increase in only anti-M2-CR Ab titres. Furthermore, anti-β1-AR, anti-β2-AR and anti-M2-CR, exhibit significantly higher prevalence in chronically T. cruzi-infected C3H/He mice when compared with C57BL/6. These observations suggest that T. cruzi infection leads to host-specific cardiac electric alterations.

Keywords

Trypanosoma cruzimuscarinic receptorsadrenergic receptorschronic Chagas disease
Type
Research Article
Information
Parasitology , Volume 141 , Issue 13 , November 2014 , pp. 1769 - 1778
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Copyright
Copyright © Cambridge University Press 2014 

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