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Morphological characterization of Cryptosporidium parvum life-cycle stages in an in vitro model system

  • H. BOROWSKI (a1), R. C. A. THOMPSON (a1), T. ARMSTRONG (a1) and P. L. CLODE (a2)
  • DOI: http://dx.doi.org/10.1017/S0031182009990837
  • Published online: 20 August 2009
Abstract
SUMMARY

Cryptosporidium parvum is a zoonotic protozoan parasite that mainly affects the ileum of humans and livestock, with the potential to cause severe enteric disease. We describe the complete life cycle of C. parvum in an in vitro system. Infected cultures of the human ileocecal epithelial cell line (HCT-8) were observed over time using electron microscopy. Additional data are presented on the morphology, development and behavioural characteristics of the different life-cycle stages as well as determining their time of occurrence after inoculation. Numerous stages of C. parvum and their behaviour have been visualized and morphologically characterized for the first time using scanning electron microscopy. Further, parasite-host interactions and the effect of C. parvum on host cells were also visualized. An improved understanding of the parasite's biology, proliferation and interactions with host cells will aid in the development of treatments for the disease.

Copyright
Corresponding author
*Corresponding author: WHO Collaborating Centre for the Molecular Epidemiology of Parasitic Infections, Veterinary and Biomedical Sciences, Murdoch University, South Street, Murdoch, WA 6150, Australia. Tel: +(08) 9360 2466. Fax: +(08) 9360 6285. E-mail: a.thompson@murdoch.edu.au
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D. A. Barnes , A. Bonnin , J. X. Huang , L. Gousset , J. Wu , J. Gut , P. Doyle , J. F. Dubremetz , H. Ward and C. Petersen (1998). A novel multi-domain mucin-like glycoprotein of Cryptosporidium parvum mediates invasion. Molecular and Biochemical Parasitology 96, 93–110.

J. R. Barta and R. C. A. Thompson (2006). What is Cryptosporidium? Reappraising its biology and phylogenetic affinities. Trends in Parasitology 22, 463468.

A. Bonnin , J. Gut , J. F. Dubremetz , R. G. Nelson and P. Camerlynck (1995). Monoclonal antibodies identify a subset of dense granules in Cryptosporidium parvum zoites and gamonts. The Journal of Eukaryotic Microbiology 42, 395401.

H. Borowski , P. L. Clode and R. C. A. Thompson (2008). Active invasion and/or encapsulation? A reappraisal of host-cell parasitism by Cryptosporidium. Trends in Parasitology 24, 509516.

R. A. Carreno , D. S. Martin and J. R. Barta (1999). Cryptosporidium is more closely related to the gregarines than to coccidia as shown by phylogenetic analysis of apicomplexan parasites inferred using small-subunit ribosomal RNA gene sequences. Parasitology Research 85, 899904.

X. M. Chen , B. Q. Huang , P. L. Splinter , J. D. Orth , D. D. Billadeau , M. A. McNiven and N. F. LaRusso (2004 a). Cdc42 and the actin-related protein/neural Wiskott-Aldrich syndrome protein network mediate cellular invasion by Cryptosporidium parvum. Infection and Immunity 72, 30113021.

X. M. Chen and N. F. LaRusso (2000). Mechanisms of attachment and internalization of Cryptosporidium parvum to biliary and intestinal epithelial cells. Gastroenterology 118, 368379.

X. M. Chen , S. P. O'Hara , B. Q. Huang , P. L. Splinter , J. B. Nelson and N. F. LaRusso (2005). Localized glucose and water influx facilitates Cryptosporidium parvum cellular invasion by means of modulation of host-cell membrane protrusion. Proceedings of the National Academy of Sciences, USA 102, 63386343.

X. M. Chen , P. L. Splinter , P. S. Tietz , B. Q. Huang , D. D. Billadeau and N. F. LaRusso (2004 b). Phosphatidylinositol 3-kinase and frabin mediate Cryptosporidium parvum cellular invasion via activation of Cdc42. The Journal of Biological Chemistry 279, 3167131678.

D. A. Elliott and D. P. Clark (2000). Cryptosporidium parvum induces host cell actin accumulation at the host-parasite interface. Infection and Immunity 68, 23152322.

A. Hashim , G. Mulcahy , B. Bourke and M. Clyne (2006). Interaction of Cryptosporidium hominis and Cryptosporidium parvum with primary human and bovine intestinal cells. Infection and Immunity 74, 99–107.

N. S. Hijjawi , B. P. Meloni , U. M. Morgan and R. C. A. Thompson (2001). Complete development and long-term maintenance of Cryptosporidium parvum human and cattle genotypes in cell culture. International Journal for Parasitology 31, 10481055.

N. S. Hijjawi , B. P. Meloni , U. M. Ryan , M. E. Olson and R. C. A. Thompson (2002). Successful in vitro cultivation of Cryptosporidium andersoni: evidence for the existence of novel extracellular stages in the life cycle and implications for the classification of Cryptosporidium. International Journal for Parasitology 32, 17191726.

N. S. Hijjawi , B. P. Meloni , M. Ng'anzo , U. M. Ryan , M. E. Olson , P. T. Cox , P. T. Monis and R. C. A. Thompson (2004). Complete development of Cryptosporidium parvum in host cell-free culture. International Journal for Parasitology 34, 769777.

B. Q. Huang , X. M. Chen and N. F. LaRusso (2004). Cryptosporidium parvum attachment to and internalization by human biliary epithelia in vitro: a morphologic study. The Journal of Parasitology 90, 212221.

Z. A. Kuznar and M. Elimelech (2006). Cryptosporidium oocyst surface macromolecules significantly hinder oocyst attachment. Environmental Science and Technology 40, 18371842.

D. Lacombe , S. Jakowska and E. Silva (2002). Gregarine Cephaloidophora communis mawrodiadi, 1908 in the barnacle Euraphia rhyzophorae, Oliveira, 1940 from Brazil. Memórias do Instituto Oswaldo Cruz 97, 10571061.

S. C. Landers (2001). Pterospora floridiensis, a new species of acephaline gregarine (Apicomplexa) from the maldanid polychaete Axiothella mucosa in St. Andrew Bay, Florida. Systematic Parasitology 48, 5559.

B. P. Meloni and R. C. A. Thompson (1996). Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro. The Journal of Parasitology 82, 757762.

P. J. O'Donoghue (1995). Cryptosporidium and cryptosporidiosis in man and animals. International Journal for Parasitology 25, 139195.

M. E. Perkins , Y. A. Riojas , T. W. Wu and S. M. Le Blancq (1999). CpABC, a Cryptosporidium parvum ATP-binding cassette protein at the host-parasite boundary in intracellular stages. Proceedings of the National Academy of Sciences, USA 96, 57345739.

R. C. Pollok , V. McDonald , P. Kelly and M. J. Farthing (2003). The role of Cryptosporidium parvum-derived phospholipase in intestinal epithelial cell invasion. Parasitology Research 90, 181186.

H. V. Smith , R. A. Nichols and A. M. Grimason (2005). Cryptosporidium excystation and invasion: getting to the guts of the matter. Trends in Parasitology 21, 133142.

W. J. Snelling , Q. Lin , J. E. Moore , B. C. Millar , F. Tosini , E. Pozio , J. S. Dooley and C. J. Lowery (2007). Proteomics analysis and protein expression during sporozoite excystation of Cryptosporidium parvum (Coccidia, Apicomplexa). Molecular and Cellular Proteomics: MCP 6, 346355.

L. Tetley , S. M. Brown , V. McDonald and G. H. Coombs (1998). Ultrastructural analysis of the sporozoite of Cryptosporidium parvum. Microbiology 144, 32493255.

R. C. A. Thompson , M. E. Olson , G. Zhu , S. Enomoto , M. S. Abrahamsen and N. S. Hijjawi (2005). Cryptosporidium and cryptosporidiosis. Advances in Parasitology 59, 77–158.

M. A. Toso and C. K. Omoto (2007). Ultrastructure of the Gregarina niphandrodes nucleus through stages from unassociated trophozoites to gamonts in syzygy and the syzygy junction. The Journal of Parasitology 93, 479484.

A. Valigurova , M. Jirku , B. Koudela , M. Gelnar , D. Modry and J. Slapeta (2008). Cryptosporidia: epicellular parasites embraced by the host cell membrane. International Journal for Parasitology 38, 913922.

D. M. Wetzel , J. Schmidt , M. S. Kuhlenschmidt , J. P. Dubey and L. D. Sibley (2005). Gliding motility leads to active cellular invasion by Cryptosporidium parvum sporozoites. Infection and Immunity 73, 53795387.

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