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    Benchimol, Marlene de Almeida, Luiz G. P. Vasconcelos, Ana Tereza de Andrade Rosa, Ivone Reis Bogo, Maurício Kist, Luiza Wilges and de Souza, Wanderley 2017. Draft Genome Sequence of Tritrichomonas foetus Strain K . Genome Announcements, Vol. 5, Issue. 16,
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New insights on the Golgi complex of Tritrichomonas foetus

  • IVONE De ANDRADE ROSA (a1) (a2) (a3), MARJOLLY BRIGIDO CARUSO (a4), SILAS PESSINI RODRIGUES (a4), REINALDO BARROS GERALDO (a4), LUIZA WILGES KIST (a5), MAURICIO REIS BOGO (a5), LUIZ GONZAGA (a6), ANA TEREZA R DE VASCONCELOS (a6), JOSE ANDRES MORGADO-DÍAZ (a7), RUSSOLINA BENEDETA ZINGALI (a4) and MARLENE BENCHIMOL (a1)...
Summary

Tritrichomonas foetus is a protist that causes bovine trichomoniasis and presents a well-developed Golgi. There are very few studies concerning the Golgi in trichomonads. In this work, monoclonal antibodies were raised against Golgi of T. foetus and used as a tool on morphologic and biochemical studies of this organelle. Among the antibodies produced, one was named mAb anti-Golgi 20.3, which recognized specifically the Golgi complex by fluorescence and electron microscopy. By immunoblotting this antibody recognized two proteins with 60 and 66 kDa that were identified as putative beta-tubulin and adenosine triphosphatase, respectively. The mAb 20.3 also recognized the Golgi complex of the Trichomonas vaginalis, a human parasite. In addition, the nucleotide coding sequences of these proteins were identified and included in the T. foetus database, and the 3D structure of the proteins was predicted. In conclusion, this study indicated: (1) adenosine triphosphatase is present in the Golgi, (2) ATPase is conserved between T. foetus and T. vaginalis, (3) there is new information concerning the nucleic acid sequences and protein structures of adenosine triphosphatase and beta-tubulin from T. foetus and (4) the mAb anti-Golgi 20.3 is a good Golgi marker and can be used in future studies.

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COPYRIGHT: © Cambridge University Press 2013 
Corresponding author
* Corresponding author: Rua Jornalista Orlando Dantas 59, Botafogo, CEP 22231-010 Rio de Janeiro, RJ, Brazil. E-mail: marlenebenchimol@gmail.com
References
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Abreu, P. A., Albuquerque, M. G., Rodrigues, C. R. and Castro, H. C. (2006). Structure function inferences based on molecular modeling, sequence-based methods and biological data analysis of snake venom lectins. Toxicon 48, 690–701.
Almeida, J. C., Benchimol, M., de Souza, W. and Okorokov, L. A. (2003). Ca(2+) sequestering in the early-branching amitochondriate protozoan Tritrichomonas foetus: an important role of the Golgi complex and its Ca(2+)-ATPase. Biochimica et Biophysica Acta 1615, 60–68.
Benchimol, M. (2004) Trichomonads under microscopy. Microscopy and Microanalysis 10, 528–550.
Benchimol, M. and Bernardino, M. V. (2002). Ultrastructural localization of glycoconjugates in Tritrichomonas foetus . Parasitology Research 88, 134–143.
Benchimol, M., Elias, C. A. and De Souza, W. (1982). Tritrichomonas foetus: fine structure of freeze-fractured membranes. Journal of Protozoology 29, 348–353.
Benchimol, M., Ribeiro, K. C., Mariante, R. M. and Alderete, J. F. (2001). Structure and division of the Golgi complex in Trichomonas vaginalis and Tritrichomonas foetus . European Journal of Cell Biology 80, 593–607.
Bozner, P. (1997). Immunological detection and subcellular localization of Hsp70 and Hsp60 homologs in Trichomonas vaginalis . Journal of Parasitology 83, 224–229.
Carafoli, E. and Brini, M. (2000). Calcium pumps: structural basis for and mechanism of calcium transmembrane transport. Current Opinion in Chemical Biology 4, 152–161.
Carlton, J. M., Hirt, R. P., Silva, J. C., Delcher, A. L., Schatz, M., Zhao, Q., Wortman, J. R., Bidwell, S. L., Alsmark, U. C., Besteiro, S., Sicheritz-Ponten, T., Noel, C. J., Dacks, J. B., Foster, P. G., Simillion, C., Van de Peer, Y., Miranda-Saavedra, D., Barton, G. J., Westrop, G. D., Müller, S., Dessi, D., Fiori, P. L., Ren, Q., Paulsen, I., Zhang, H., Bastida-Corcuera, F. D., Simoes-Barbosa, A., Brown, M. T., Hayes, R. D., Mukherjee, M., Okumura, C. Y., Schneider, R., Smith, A. J., Vanacova, S., Villalvazo, M., Haas, B. J., Pertea, M., Feldblyum, T. V., Utterback, T. R., Shu, C. L., Osoegawa, K., de Jong, P. J., Hrdy, I., Horvathova, L., Zubacova, Z., Dolezal, P., Malik, S. B., Logsdon, J. M. Jr., Henze, K., Gupta, A., Wang, C. C., Dunne, R. L., Upcroft, J. A., Upcroft, P., White, O., Salzberg, S. L., Tang, P., Chiu, C. H., Lee, Y. S., Embley, T. M., Coombs, G. H., Mottram, J. C., Tachezy, J., Fraser-Liggett, C. M. and Johnson, P. J. (2007). Draft genome sequence of the sexually transmitted pathogen Trichomonas vaginalis . Science 315, 207–212.
Castro, H. C., Silva, D. M., Craik, C. and Zingali, R. B. (2001 ). Structural features of a snake venom thrombin-like enzyme: thrombin and trypsin on a single catalytic platform? Biochemica Biophysica Acta 1547, 183–195.
De Melo, E. J. and de Souza, W. (1996). Pathway of C6-NBD-Ceramide on the host cell infected with Toxoplasma gondii . Cell Structure and Function 21, 47–52.
Diamond, L. S. (1957). The establishment of various trichomonads of animals and man in axenic cultures. Journal of Parasitology 43, 488–490.
Díaz, J. A. and De Souza, W. (1998). Biochemical characterization of the Golgi-complex proteins of Tritrichomonas foetus . Parasitology Research 84, 760–762.
Díaz, J. A., Monteiro-Leal, L. H. and De Souza, W. (1996). Tritrichomonas foetus: isolation and characterization of the Golgi complex. Experimental Parasitology 83, 174–183.
Eng, J. K., McCormack, A. L. and Yates, J. R. J. III (1994). An approach to correlate tandem mass spectral data of peptides with amino acid sequence in a protein database. American Society for Mass Spectrometry 5, 976–989.
Gookin, J. L., Stebbins, M. E., Hunt, E., Burlone, K., Fulton, M., Hochel, R., Talaat, M., Poore, M. and Levy, M. G. (2004). Prevalence of and risk factors for feline Tritrichomonas foetus and giardia infection. Journal of Clinical Microbiology 42, 2707–2710.
Grunow, A., Rüsing, M., Becker, B. and Melkonian, M. (1999). V-ATPase is a major component of the Golgi complex in the scaly green flagellate Scherffeli adubia . Protist 150, 265–281.
Hampl, V., Pavlícek, A. and Flegr, J. (2001). Construction and bootstrap analysis of DNA finger printing-based phylogenetic trees with the freeware program FreeTree: application to trichomonad parasites. International Journal of Systematic and Evolutionary Microbiology 51, 731–735.
Honigberg, B. M. and Brugerolle, G. (1990). Structure. In Trichomonads Parasitic in Humans (ed. Honigberg, B. M.), pp. 54–83. Springer-Verlag, New York, USA.
Honigberg, B. M., Mattern, C. F. T. and Daniel, W. A. (1971). Fine structure of the mastigont system in Tritrichomonas foetus (Riedmuller). Journal of Protozoology 18, 183–192.
Knudsen, G. M. and Chalkley, R. J. (2011). The effect of using an inappropriate protein database for proteomic data analysis. PLoS ONE 6, e20873.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.
Lindmark, D. G. and Müller, M. (1973). Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritrichomonas foetus, and its role in pyruvate metabolism. Journal of Biological Chemistry 248, 7724–7728.
Lopes, L. C., Ribeiro, K. C. and Benchimol, M. (2001). Immunolocalization of tubulin isoforms and post-translational modifications in the protists Tritrichomonas foetus and Trichomonas vaginalis . Histochemistry and Cell Biology 116, 17–29.
Lucocq, J. M. and Gawden-Bone, C. (2010). Quantitative assessment of specificity in immunoelectron microscopy. Journal of Histochemistry and Cytochemistry 58, 917–927.
Lundgren, D. H., Han, D. K. and Eng, J. K. (2005). Unit 13.3. Protein identification using TurboSEQUEST. Current Protocols in Bioinformatics. doi: 10.1002/0471250953.bi1303s10.
Lytton, J., Westlin, M., Burk, S. E., Shull, G. E. and MacLennan, D. H. (1992). Functional comparisons between isoforms of the sarcoplasmic or endoplasmic reticulum family of calcium pumps. Journal of Biological Chemistry 267, 14483–14489.
Madeiro da Costa, R. F. and Benchimol, M. (2004). The effect of drugs on cell structure of Tritrichomonas foetus . Parasitology Research 92, 159–170.
Müller, M. (1973). Biochemical cytology of trichomonad flagellates. I. Subcellular localization of hydrolases, dehydrogenases, and catalase in Tritrichomonas foetus . Journal of Cell Biology 57, 453–474.
Nielsen, M. H. (1974). Fine structural localization of nucleoside triphosphatase and acid phosphatase activity in Trichomonas vaginalis Donné. Cell and Tissue Research 151, 269–280.
Ordenes, V. R., Reyes, F. C., Wolff, D. and Orellana, A. (2002). A thapsigargin-sensitive Ca(2+) pump is present in the pea Golgi apparatus membrane. Plant Physiology 129, 1820–1828.
Pagano, R. E., Sepanski, M. A. and Martin, O. C. (1989). Molecular trapping of a fluorescent ceramide analogue at the Golgi apparatus of fixed cells: interaction with endogenous lipids provides a trans-Golgi marker for both light and electron microscopy. Journal of Cell Biology 109, 2067–2079.
Queiroz, R. C. B., Santos, L. M. S., Benchimol, M. and De Souza, W. (1991). Cytochemical localization of enzyme markers in Tritrichomonas foetus . Parasitology Research 77, 561–566.
Rae, D. O. and Crews, J. E. (2006). Tritrichomonas foetus . Veterinary Clinics of North America. Food Animal Practice 22, 595–611.
Rodrigues, S. P., Ventura, J. A., Aguilar, C., Nakayasu, E. S., Almeida, I. C., Fernandes, P. M. and Zingali, R. B. (2011). Proteomic analysis of papaya (Carica papaya L.) displaying typical sticky disease symptoms. Proteomics 11, 2592–2602.
Sagara, Y. and Inesi, G. (1991). Inhibition of the sarcoplasmic reticulum Ca2+ transport ATPase by thapsigargin at subnanomolar concentrations. Journal of Biological Chemistry 266, 13503–13506.
Sharma, N. N. and Bourne, G. H. (1967). Distribution of adenosine triphosphatase in Trichomonas vaginalis . Acta Histochemica 26, 210–221.
Simons, K. and van Meer, G. (1988). Lipid sorting in epithelial cells. Biochemistry 27, 6197–6202.
Stockdale, H. D., Dillon, A. R., Newton, J. C., Bird, R. C., Bondurant, R. H., Deinnocentes, P., Barney, S., Bulter, J., Land, T., Spencer, J. A., Lindsay, D. S. and Blagburn, B. L. (2008). Experimental infection of cats (Felis catus) with Tritrichomonas foetus isolated from cattle. Veterinary Parasitology 154, 156–161.
Yaeger, M. J. and Gookin, J. L. (2005). Histologic features associated with Tritrichomonas foetus-induced colitis in domestic cats. Veterinary Pathology 42, 797–804.
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Parasitology
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