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A novel miRNA, miR-13664, targets CpCYP314A1 to regulate deltamethrin resistance in Culex pipiens pallens

Published online by Cambridge University Press:  03 July 2018

X. H. Sun ,
N. Xu Open the ORCID record for N. Xu [Opens in a new window] ,
Y. Xu ,
D. Zhou ,
Y. Sun ,
W. J. Wang ,
L. Ma ,
C. L. Zhu  and
B. Shen
X. H. Sun
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
N. Xu
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
Y. Xu
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
D. Zhou
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
Y. Sun
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
W. J. Wang
Affiliation:
Department of Pathogen Biology, Hebei Medical University, Shijiazhuang 050017, China
L. Ma
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
C. L. Zhu*
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
B. Shen*
Affiliation:
Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu 211166, PR China Jiangsu Province Key Laboratory of Modern Pathogen Biology, Nanjing, Jiangsu 211166, PR China
*
Author for correspondence: B. Shen, E-mail: shenbo@njmu.edu.cn and C.L. Zhu, clzhu@njmu.edu.cn
Author for correspondence: B. Shen, E-mail: shenbo@njmu.edu.cn and C.L. Zhu, clzhu@njmu.edu.cn
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Abstract

Extensive insecticide use has led to the resistance of mosquitoes to these insecticides, posing a major barrier to mosquito control. Previous Solexa high-throughput sequencing of Culex pipiens pallens in the laboratory has revealed that the abundance of a novel microRNA (miRNA), miR-13664, was higher in a deltamethrin-sensitive (DS) strain than a deltamethrin-resistant (DR) strain. Real-time quantitative PCR revealed that the miR-13664 transcript level was lower in the DR strain than in the DS strain. MiR-13664 oversupply in the DR strain increased the susceptibility of these mosquitoes to deltamethrin, whereas inhibition of miR-13664 made the DS strain more resistant to deltamethrin. Results of bioinformatic analysis, quantitative reverse-transcriptase polymerase chain reaction, luciferase assay and miR mimic/inhibitor microinjection revealed CpCYP314A1 to be a target of miR-13664. In addition, downregulation of CpCYP314A1 expression in the DR strain reduced the resistance of mosquitoes to deltamethrin. Taken together, our results indicate that miR-13664 could regulate deltamethrin resistance by interacting with CpCYP314A1, providing new insights into mosquito resistance mechanisms.

Keywords

Culex pipiens pallensdeltamethrin resistanceCpCYP314A1miR-13664
Type
Research Article
Information
Parasitology , Volume 146 , Issue 2 , February 2019 , pp. 197 - 205
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Copyright
Copyright © Cambridge University Press 2018 

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