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Paramyosin from the parasitic mite Sarcoptes scabiei: cDNA cloning and heterologous expression

  • J. G. MATTSSON (a1), E. L. LJUNGGREN (a1) and K. BERGSTRÖM (a1)

The burrowing mite Sarcoptes scabiei is the causative agent of the highly contagious disease sarcoptic mange or scabies. So far, there is no in vitro propagation system for S. scabiei available, and mites used for various purposes must be isolated from infected hosts. Lack of parasite-derived material has limited the possibilities to study several aspects of scabies, including pathogenesis and immunity. It has also hampered the development of high performance serological assays. We have now constructed an S. scabiei cDNA expression library with mRNA purified from mites isolated from red foxes. Immunoscreening of the library enabled us to clone a full-length cDNA coding for a 102.5 kDa protein. Sequence similarity searches identified the protein as a paramyosin. Recombinant S. scabiei paramyosin expressed in Escherichia coli was recognized by sera from dogs and swine infected with S. scabiei. We also designed a small paramyosin construct of about 17 kDa that included the N-terminal part, an evolutionary variable part of the helical core, and the C-terminal part of the molecule. The miniaturized protein was efficiently expressed in E. coli and was recognized by sera from immunized rabbits. These data demonstrate that the cDNA library can assist in the isolation of important S. scabiei antigens and that recombinant proteins can be useful for the study of scabies.

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ARLIAN, L. G. (1989). Biology, host relations, and epidemiology of Sarcoptes scabiei. Annual Review of Entomology34, 139161.

ARLIAN, L. G., MORGAN, M. S., VYSZENSKI-MOHER, D. L. & STEMMER, B. L. (1994). Sarcoptes scabiei: the circulating antibody response and induced immunity to scabies. Experimental Parasitology78, 3750.

BECKER, K. D., O'DONNELL, P. T., HEITZ, J. M., VITO, M. & BERNSTEIN, S. I. (1992). Analysis of Drosophila paramyosin: identification of a novel isoform which is restricted to a subset of adult muscles. Journal of Cell Biology116, 669681.

BORNSTEIN, S., THEBO, P. & ZAKRISSON, G. (1996). Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of canine sarcoptic mange. Veterinary Dermatology7, 2128.

BORNSTEIN, S. & ZAKRISSON, G. (1993). Humoral antibody response to experimental Sarcoptes scabiei var. vulpes infection in the dog. Veterinary Dermatology4, 107110.

BURGESS, I. (1994). Sarcoptes scabiei and scabies. Advances in Parasitology33, 235292.

CHOSIDOW, O. (2000). Scabies and pediculosis. Lancet355, 819826.

DAHMEN, A., GALLIN, M., SCHUMACHER, M. & ERTTMANN, K. D. (1993). Molecular cloning and pre-mRNA maturation of Onchocerca volvulus paramyosin. Molecular and Biochemical Parasitology57, 335338.

DEVEREUX, J., HAEBERLI, P. & SMITHIES, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research12, 387395.

HOLLANDERS, W., VERCRUYSSE, J., RAES, S. & BORNSTEIN, S. (1997). Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of sarcoptic mange in swine. Veterinary Parasitology69, 117123.

JACOBS, E. & CLAD, A. (1986). Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Analytical Biochemistry154, 583589.

KALINNA, B. H. & MCMANUS, D. P. (1997). A vaccine against the Asian schistosome, Schistosoma japonicum: an update on paramyosin as a target of protective immunity. International Journal for Parasitology27, 12131219.

LACLETTE, J. P., LANDA, A., ARCOS, L., WILLMS, K., DAVIS, A. E. & SHOEMAKER, C. B. (1991). Paramyosin is the Schistosoma mansoni (Trematoda) homologue of antigen B from Taenia solium (Cestoda). Molecular and Biochemical Parasitology44, 287295.

LANDA, A., LACLETTE, J. P., NICHOLSON-WELLER, A. & SHOEMAKER, C. B. (1993). cDNA cloning and recombinant expression of collagen-binding and complement inhibitor activity of Taenia solium paramyosin (AgB). Molecular and Biochemical Parasitology60, 343347.

LIMBERGER, R. J. & MCREYNOLDS, L. A. (1990). Filarial paramyosin: cDNA sequences from Dirofilaria immitis and Onchocerca volvulus. Molecular and Biochemical Parasitology38, 271280.

MÖRNER, T. (1992). Sarcoptic mange in Swedish wildlife. Revue Scientific et technique Office International des Epizooties11, 11151121.

PRYOR, K. D. & LEITING, B. (1997). High-level expression of soluble protein in Escherischia coli using a His6-tag and maltose-binding-protein double-affinity fusion system. Protein Expression and Purification10, 309319.

SCHLESINGER, I., OELRICH, D. M. & TYRING, S. K. (1994). Crusted (Norwegian) scabies in patients with AIDS: the range of clinical presentations. Southern Medical Journal87, 352356.

TELLAM, R. L. & BOWLES, V. M. (1997). Control of blowfly strike in sheep: current strategies and future prospects. International Journal for Parasitology27, 261273.

WILLADSEN, P. (1997). Novel vaccines for ectoparasites. Veterinary Parasitology71, 209222.

ZAHLER, M., ESSIG, A., GOTHE, R. & RINDER, H. (1999). Molecular analyses suggest monospecificity of the genus Sarcoptes (Acari: Sarcoptidae). International Journal for Parasitology29, 759766.

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  • ISSN: 0031-1820
  • EISSN: 1469-8161
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