Skip to main content
×
Home
    • Aa
    • Aa

Paramyosin from the parasitic mite Sarcoptes scabiei: cDNA cloning and heterologous expression

  • J. G. MATTSSON (a1), E. L. LJUNGGREN (a1) and K. BERGSTRÖM (a1)
Abstract

The burrowing mite Sarcoptes scabiei is the causative agent of the highly contagious disease sarcoptic mange or scabies. So far, there is no in vitro propagation system for S. scabiei available, and mites used for various purposes must be isolated from infected hosts. Lack of parasite-derived material has limited the possibilities to study several aspects of scabies, including pathogenesis and immunity. It has also hampered the development of high performance serological assays. We have now constructed an S. scabiei cDNA expression library with mRNA purified from mites isolated from red foxes. Immunoscreening of the library enabled us to clone a full-length cDNA coding for a 102.5 kDa protein. Sequence similarity searches identified the protein as a paramyosin. Recombinant S. scabiei paramyosin expressed in Escherichia coli was recognized by sera from dogs and swine infected with S. scabiei. We also designed a small paramyosin construct of about 17 kDa that included the N-terminal part, an evolutionary variable part of the helical core, and the C-terminal part of the molecule. The miniaturized protein was efficiently expressed in E. coli and was recognized by sera from immunized rabbits. These data demonstrate that the cDNA library can assist in the isolation of important S. scabiei antigens and that recombinant proteins can be useful for the study of scabies.

Copyright
Corresponding author
Corresponding author. Tel: +46 18 67 41 20. Fax: +46 18 30 91 62. E-mail: Jens.Mattsson@sva.se
References
Hide All
ARLIAN, L. G. (1989). Biology, host relations, and epidemiology of Sarcoptes scabiei. Annual Review of Entomology 34, 139161.
ARLIAN, L. G., MORGAN, M. S., VYSZENSKI-MOHER, D. L. & STEMMER, B. L. (1994). Sarcoptes scabiei: the circulating antibody response and induced immunity to scabies. Experimental Parasitology 78, 3750.
BECKER, K. D., O'DONNELL, P. T., HEITZ, J. M., VITO, M. & BERNSTEIN, S. I. (1992). Analysis of Drosophila paramyosin: identification of a novel isoform which is restricted to a subset of adult muscles. Journal of Cell Biology 116, 669681.
BORNSTEIN, S., THEBO, P. & ZAKRISSON, G. (1996). Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of canine sarcoptic mange. Veterinary Dermatology 7, 2128.
BORNSTEIN, S. & WALLGREN, P. (1997). Serodiagnosis of sarcoptic mange in pigs. Veterinary Record 141, 812.
BORNSTEIN, S. & ZAKRISSON, G. (1993). Humoral antibody response to experimental Sarcoptes scabiei var. vulpes infection in the dog. Veterinary Dermatology 4, 107110.
BURGESS, I. (1994). Sarcoptes scabiei and scabies. Advances in Parasitology 33, 235292.
CHOSIDOW, O. (2000). Scabies and pediculosis. Lancet 355, 819826.
DAHMEN, A., GALLIN, M., SCHUMACHER, M. & ERTTMANN, K. D. (1993). Molecular cloning and pre-mRNA maturation of Onchocerca volvulus paramyosin. Molecular and Biochemical Parasitology 57, 335338.
DEVEREUX, J., HAEBERLI, P. & SMITHIES, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12, 387395.
HOLLANDERS, W., VERCRUYSSE, J., RAES, S. & BORNSTEIN, S. (1997). Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of sarcoptic mange in swine. Veterinary Parasitology 69, 117123.
JACOBS, E. & CLAD, A. (1986). Electroelution of fixed and stained membrane proteins from preparative sodium dodecyl sulfate-polyacrylamide gels into a membrane trap. Analytical Biochemistry 154, 583589.
KALINNA, B. H. & MCMANUS, D. P. (1997). A vaccine against the Asian schistosome, Schistosoma japonicum: an update on paramyosin as a target of protective immunity. International Journal for Parasitology 27, 12131219.
LACLETTE, J. P., LANDA, A., ARCOS, L., WILLMS, K., DAVIS, A. E. & SHOEMAKER, C. B. (1991). Paramyosin is the Schistosoma mansoni (Trematoda) homologue of antigen B from Taenia solium (Cestoda). Molecular and Biochemical Parasitology 44, 287295.
LANDA, A., LACLETTE, J. P., NICHOLSON-WELLER, A. & SHOEMAKER, C. B. (1993). cDNA cloning and recombinant expression of collagen-binding and complement inhibitor activity of Taenia solium paramyosin (AgB). Molecular and Biochemical Parasitology 60, 343347.
LIMBERGER, R. J. & MCREYNOLDS, L. A. (1990). Filarial paramyosin: cDNA sequences from Dirofilaria immitis and Onchocerca volvulus. Molecular and Biochemical Parasitology 38, 271280.
MÖRNER, T. (1992). Sarcoptic mange in Swedish wildlife. Revue Scientific et technique Office International des Epizooties 11, 11151121.
NORMAZNAH, Y., SANIAH, K., NAZMA, M., MAK, J. W., KRISHNASAMY, M. & HAKIM, S. L. (1996). Seroprevalence of Sarcoptes scabiei var. canis antibodies among aborigines in peninsular Malaysia. Southeast Asian Journal of Tropical Medicine and Public Health 27, 5356.
PRYOR, K. D. & LEITING, B. (1997). High-level expression of soluble protein in Escherischia coli using a His6-tag and maltose-binding-protein double-affinity fusion system. Protein Expression and Purification 10, 309319.
RIGGS, P. (1997). Detection and isolation of recombinant protein based on binding affinity reporter. Maltose binding protein. Methods in Molecular Biology 63, 85101.
SCHLESINGER, I., OELRICH, D. M. & TYRING, S. K. (1994). Crusted (Norwegian) scabies in patients with AIDS: the range of clinical presentations. Southern Medical Journal 87, 352356.
TELLAM, R. L. & BOWLES, V. M. (1997). Control of blowfly strike in sheep: current strategies and future prospects. International Journal for Parasitology 27, 261273.
WALTON, S. F., CHOY, J. L., BONSON, A., VALLE, A., MCBROOM, J., TAPLIN, D., ARLIAN, L., MATHEWS, J. D., CURRIE, B. & KEMP, D. J. (1999). Genetically distinct dog-derived and human-derived Sarcoptes scabiei in scabies-endemic communities in northern Australia. American Journal of Tropical Medicine and Hygiene 61, 542547.
WILLADSEN, P. (1997). Novel vaccines for ectoparasites. Veterinary Parasitology 71, 209222.
ZAHLER, M., ESSIG, A., GOTHE, R. & RINDER, H. (1999). Molecular analyses suggest monospecificity of the genus Sarcoptes (Acari: Sarcoptidae). International Journal for Parasitology 29, 759766.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Metrics

Full text views

Total number of HTML views: 1
Total number of PDF views: 10 *
Loading metrics...

Abstract views

Total abstract views: 107 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 22nd October 2017. This data will be updated every 24 hours.