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Quantitative analysis of parasite DNA in the blood of immunized and naïve mice after infection with Neospora caninum

  • S. PINITKIATISAKUL (a1), J. G. MATTSSON (a1) and A. LUNDÉN* (a1)
Summary

Real-time PCR was used to study the duration and level of parasitaemia in mice immunized with immune-stimulating complexes (iscoms) containing recombinant NcSRS2, one of the immunodominant surface antigens of Neospora caninum. After challenge infection, blood was collected daily for 9 days. During this period the amounts of parasite DNA detected in immunized mice were significantly lower (P<0·001), and the duration of parasitaemia appeared to be shorter, than in non-immunized controls. Furthermore, the degree of parasitaemia seemed to correlate well with the amount of N. caninum DNA in the brain 3 weeks post-inoculation and with disease severity measured as changes in body weight. These results indicate that the protective immunity induced by the NcSRS2-iscoms was sufficient to reduce the level of parasitaemia, which probably reduced the number of parasites reaching the brain, and could be the reason for the reduction in brain parasite load and clinical symptoms. Furthermore, real-time PCR was found to be a sensitive means for rapid assessment of N. caninum in blood.

Copyright
COPYRIGHT: © Cambridge University Press 2007
Corresponding author
*Corresponding author. Tel: +46 18 674000. Fax: +46 18 309162. E-mail: Anna.Lunden@bvf.slu.se
References
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Alaeddine, F., Keller, N., Leepin, A. and Hemphill, A. (2005). Reduced infection and protection from clinical signs of cerebral neosporosis in C57BL/6 mice vaccinated with recombinant microneme antigen NcMIC1. Journal of Parasitology 91, 657–665.
Baszler, T. V., Long, M. T., McElwain, T. F. and Mathison, B. A. (1999). Interferon-γ and interleukin-12 mediate protection to acute Neospora caninum infection in BALB/c mice. International Journal for Parasitology 29, 1635–1646.
Cannas, A., Naguleswaran, A., Müller, N., Gottstein, B. and Hemphill, A. (2003). Reduced cerebral infection of Neospora caninum-infected mice after vaccination with recombinant microneme protein NcMIC3 and RIBI adjuvant. Journal of Parasitology 89, 44–50.
Collantes-Fernández, E., López-Pérez, I., Álvarez-Garcia, G. and Ortega-Mora, L. M. (2006). Temporal distribution and parasite load kinetics in blood and tissues during Neospora caninum infection in mice. Infection and Immunity 74, 2491–2494.
Collantes-Fernández, E., Zaballos, A., Álvarez-Garcia, G. and Ortega-Mora, L. M. (2002). Quantitative detection of Neospora caninum in bovine aborted fetuses and experimentally infected mice by real-time PCR. Journal of Clinical Microbiology 40, 1194–1198.
Dubey, J. P., Buxton, D. and Wouda, W. (2006). Pathogenesis of bovine neosporosis. Journal of Comparative Pathology 134, 267–289.
Dubey, J. P., Dorough, K. R., Jenkins, M. C., Liddell, S., Speer, C. A., Kwok, O. C. and Shen, S. K. (1998). Canine neosporosis: clinical signs, diagnosis, treatment and isolation of Neospora caninum in mice and cell culture. International Journal for Parasitology 28, 1293–1304.
Ferre, I., Aduriz, G., Del-Pozo, I., Regidor-Cerrillo, J., Atxaerandio, R., Collantes-Fernández, E., Hurtado, A., Ugarte-Garagalza, C. and Ortega-Mora, L. M. (2005). Detection of Neospora caninum in the semen and blood of naturally infected bulls. Theriogenology 63, 1504–1518.
Innes, E. A., Andrianarivo, A. G., Björkman, C., Williams, D. J. L. and Conrad, P. A. (2002). Immune responses to Neospora caninum and prospects for vaccination. Trends in Parasitology 18, 497–504.
Kirisits, M. J., Mui, E. and McLeod, R. (2000). Measurement of the efficacy of vaccines and antimicrobial therapy against infection with Toxoplasma gondii. International Journal for Parasitology 30, 149–155.
Liddell, S., Jenkins, M. C. and Dubey, J. P. (1999). A competitive PCR assay for quantitative detection of Neospora caninum. International Journal for Parasitology 29, 1583–1587.
Lindsay, D. S., Lenz, S. D., Cole, R. A., Dubey, J. P. and Blagburn, B. L. (1995). Mouse model for central nervous system Neospora caninum infections. Journal of Parasitology 81, 313–315.
Long, M. T., Baszler, T. V. and Mathison, B. A. (1998). Comparison of intracerebral parasite load, lesion development, and systemic cytokines in mouse strains infected with Neospora caninum. Journal of Parasitology 84, 316–320.
Lundén, A., Marks, J., Maley, S. W. and Innes, E. A. (1998). Cellular immune responses in cattle experimentally infected with Neospora caninum. Parasite Immunology 20, 519–526.
Macaldowie, C., Maley, S. W., Wright, S., Bartley, P., Esteban-Redondo, I., Buxton, D. and Innes, E. A. (2004). Placental pathology associated with fetal death in cattle inoculated with Neospora caninum by two different routes in early pregnancy. Journal of Comparative Pathology 131, 142–156.
Müller, N., Vonlaufen, N., Gianinazzi, C., Leib, S. L. and Hemphill, A. (2002). Application of real-time fluorescent PCR for quantitative assessment of Neospora caninum infections in organotypic slice cultures of rat central nervous system tissue. Journal of Clinical Microbiology 40, 252–255.
Nishikawa, Y., Inoue, N., Xuan, X., Nagasawa, H., Igarashi, I., Fujisaki, K., Otsuka, H. and Mikami, T. (2001). Protective efficacy of vaccination by recombinant vaccinia virus against Neospora caninum infection. Vaccine 19, 1381–1390.
Okeoma, C. M., Stowell, K. M., Williamson, N. B. and Pomroy, W. E. (2005). Neospora caninum: quantification of DNA in the blood of naturally infected aborted and pregnant cows using real-time PCR. Experimental Parasitology 110, 48–55.
Pinitkiatisakul, S., Friedman, M., Wikman, M., Mattsson, J. G., Lövgren-Bengtsson, K., Ståhl, S. and Lundén, A. (2007). Immunogenicity and protective effect against murine cerebral neosporosis of recombinant NcSRS2 in different iscom formulations. Vaccine 25, 3658–3668.
Pinitkiatisakul, S., Mattsson, J. G., Wikman, M., Friedman, M., Bengtsson, K. L., Ståhl, S. and Lundén, A. (2005). Immunisation of mice against neosporosis with recombinant NcSRS2 iscoms. Veterinary Parasitology 129, 25–34.
Staska, L. M., McGuire, T. C., Davies, C. J., Lewin, H. A. and Baszler, T. V. (2003). Neospora caninum-infected cattle develop parasite-specific CD4+ cytotoxic T lymphocytes. Infection and Immunity 71, 3272–3279.
Wikman, M., Friedman, M., Pinitkiatisakul, S., Hemphill, A., Lövgren-Bengtsson, K., Lundén, A. and Ståhl, S. (2005). Applying biotin-streptavidin binding for iscom (immunostimulating complex) association of recombinant immunogens. Biotechnology and Applied Biochemistry 41, 163–174.
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