Skip to main content Accessibility help
×
×
Home

Recombinant protein Bd37 protected gerbils against heterologous challenges with isolates of Babesia divergens polymorphic for the bd37 gene

  • K. HADJ-KADDOUR (a1), B. CARCY (a1), A. VALLET (a1), S. RANDAZZO (a1), S. DELBECQ (a1), J. KLEUSKENS (a2), T. SCHETTERS (a1) (a2), A. GORENFLOT (a1) and E. PRECIGOUT (a1)...
Abstract

The Bd37gene encoding for a glycosyl-phosphatidyl-inositol anchored protein of Babesia divergens displays genetic polymorphisms among isolates. Five major polymorphic groups (clades) were shown by PCR-RFLP among different B. divergens isolates. Each group has been characterized according to a reference Bd37 gene (Rouen87, W8843, Y5, 6303E and 1705B). Recombinant (GST fusion) protein (Bd37r) expressed from the Bd37 gene, was used as antigen in a saponin-based formulation and was able to protect gerbils, after 2 injections at low dose vaccine concentration (1 μg per dose), against a virulent challenge with the B. divergens Rouen87 isolate. In spite of polymorphism of Bd37 gene, Bd37r induced complete immunoprotection against challenges with each of the 5 reference isolate groups defined by PCR-RFLP.

Copyright
Corresponding author
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 ‘Vaccination anti-parasitaire’ UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France. Tel: +04 67 66 81 20. Fax: +04 67 66 81 29. E-mail: kamel.hadj-kaddour@univ-montp1.fr
References
Hide All

REFERENCES

Beattie, J. F., Michelson, M. L. and Holman, P. J. ( 2002). Acute babesiosis caused by Babesia divergens in a resident of Kentucky. New England Journal of Medicine 347, 697698.
Ben Musa, N. and Dawoud, H. A. ( 2004). The protective activity of serum and fractionated serum from rats against Babesia divergens. Journal of the Egyptian Society of Parasitology 34, 407422.
Brown, W. C., Norimine, J., Goff, W. L., Suarez, C. E. and McElwain, T. F. ( 2006). Prospects for recombinant vaccines against Babesia bovis and related parasites. Parasite Immunology 28, 315327.
Carcy, B., Precigout, E., Valentin, A., Gorenflot, A. and Schrevel, J. ( 1995). A 37-kilodalton glycoprotein of Babesia divergens is a major component of a protective fraction containing low-molecular-mass culture-derived exoantigens. Infection and Immunity 63, 811817.
Christensson, D. A. ( 1987). Clinical and serological response after experimental inoculation with Babesia divergens of newborn calves with and without maternal antibodies. Acta Veterinaria Scandinavica 28, 381392.
Christensson, D. A. ( 1989). Inverse age resistance to experimental Babesia divergens infection in cattle. Acta Veterinaria Scandinavica 30, 453464.
Daly, T. M. and Long, C. A. ( 1996). Influence of adjuvants on protection induced by a recombinant fusion protein against malarial infection. Infection and Immunity 64, 26022608.
Delbecq, S., Precigout, E., Vallet, A., Carcy, B., Schetters, T. P. and Gorenflot, A. ( 2002). Babesia divergens: cloning and biochemical characterization of Bd37. Parasitology 125, 305312.
Goethert, H. K. and Telford, S. R. 3rd. ( 2003). Enzootic transmission of Babesia divergens among cottontail rabbits on Nantucket Island, Massachusetts. American Journal of Tropical Medicine and Hygiene 69, 455460.
Gorenflot, A., Brasseur, P., Precigout, E., L'Hostis, M., Marchand, A. and Schrevel, J. ( 1991). Cytological and immunological responses to Babesia divergens in different hosts: ox, gerbil, man. Parasitology Research 77, 312.
Gorenflot, A., Moubri, K., Precigout, E., Carcy, B. and Schetters, T. P. ( 1998). Human babesiosis. Annals of Tropical Medicine and Parasitology 92, 489501.
Gorenflot, A., Precigout, E., Bissuel, G., Lecointre, O., Brasseur, P., Vidor, E., L'Hostis, M. and Schrevel, J. ( 1990). Identification of major Babesia divergens polypeptides that induce protection against homologous challenge in gerbils. Infection and Immunity 58, 40764082.
Gorenflot, A., Precigout, E., Valentin, A., Bissuel, G., Carcy, B., Brasseur, P., Moreau, Y. and Schrevel, J. ( 1992). Babesia divergens vaccine. Memorias do Instituto Oswaldo Cruz 87 (Suppl. 3), 279281.
Gray, J. S. and Harte, L. N. ( 1985). An estimation of the prevalence and economic importance of clinical babesiosis in the republic of Ireland. Ireland Veterinary Journal 69, 455460.
Herwaldt, B., Persing, D. H., Precigout, E., Goff, W. L., Mathiesen, D. A., Taylor, P. W., Eberhard, M. L. and Gorenflot, A. ( 1996). A fatal case of babesiosis in Missouri: identification of another piroplasm that infects humans. Annals of Internal Medicine 124, 643650.
Herwaldt, B. L., de Bruyn, G., Pieniazek, N. J., Homer, M., Lofy, K. H., Slemenda, S. B., Fritsche, T. R., Persing, D. H. and Limaye, A. P. ( 2004). Babesia divergens-like infection, Washington State. Emerging Infectious Diseases 10, 622629.
Holman, P. J., Spencer, A. M., Droleskey, R. E., Goethert, H. K. and Telford, S. R. 3rd. ( 2005). In vitro cultivation of a zoonotic Babesia sp. isolated from eastern cottontail rabbits (Sylvilagus floridanus) on Nantucket Island, Massachusetts. Journal of Clinical Microbiology 8, 39954001.
James, M. A. ( 1989). Application of exoantigens of Babesia and Plasmodium in vaccine development. Transactions of the Royal Society of Tropical Medicine and Hygiene 83 (Suppl.), 6772.
Joyner, L. P. and Davies, S. F. ( 1967). Acquired resistance to Babesia divergens in experimental calves. Journal of Protozoology 14, 260262.
Kumar, S., Collins, W., Egan, A., Yadava, A., Garraud, O., Blackman, M. J., Guevara Patino, J. A., Diggs, C. and Kaslow, D. C. ( 2000). Immunogenicity and efficacy in aotus monkeys of four recombinant Plasmodium falciparum vaccines in multiple adjuvant formulations based on the 19-kilodalton C terminus of merozoite surface protein 1. Infection and Immunity 68, 22152223.
Kuttler, K. L. ( 1988). World-wide impact of babesiosis. In Babesiosis of Domestic Animals and Man (ed. Ristic, M.), pp. 122. CRC Press, Inc., Boca Raton, Florida.
Lewis, D. and Williams, H. ( 1979). Infection of the Mongolian gerbil with the cattle piroplasm Babesia divergens. Nature, London 278, 170171.
L'Hostis, M., Chauvin, A., Valentin, A., Precigout, E. and Gorenflot, A. ( 1997). Survey of Babesia divergens antibody kinetics in cattle in western France. Veterinary Research 28, 481848.
Montenegro-James, S., Toro Benitez, M., Leon, E., Lopez, R. and Ristic, M. ( 1987). Bovine babesiosis: induction of protective immunity with culture-derived Babesia bovis and Babesia bigemina immunogens. Parasitology Research 74, 142150.
Montenegro-James, S., Toro, M., Leon, E. and Guillen, A. T. ( 1992). Field evaluation of an exoantigen-containing Babesia vaccine in Venezuela. Memorias do Instituto Oswaldo Cruz 87 (Suppl.) 3, 283288.
Norimine, J., Mosqueda, J., Suarez, C., Palmer, G. H., McElwain, T. F., Mbassa, G. and Brown, W. C. ( 2003). Stimulation of T-helper cell gamma interferon and immunoglobulin G responses specific for Babesia bovis rhoptry-associated protein 1 (RAP-1) or a RAP-1 protein lacking the carboxy-terminal repeat region is insufficient to provide protective immunity against virulent B. bovis challenge. Infection and Immunity 71, 50215032.
Orinda, G. O., Gale, K. R., Wright, I. G. and Parrodi, F. ( 1992). Bovine babesiosis: failure to induce interferon gamma production in response to Babesia bovis antigens in cattle. International Journal for Parasitology 22, 395398.
Precigout, E., Delbecq, S., Vallet, A., Carcy, B., Camillieri, S., Hadj-Kaddour, K., Kleuskens, J., Schetters, T. and Gorenflot, A. ( 2004). Association between sequence polymorphism in an epitope of Babesia divergens Bd37 exoantigen and protection induced by passive transfer. International Journal for Parasitology 34, 585593.
Precigout, E., Gorenflot, A., Valentin, A., Bissuel, G., Carcy, B., Brasseur, P., Moreau, Y. and Schrevel, J. ( 1991). Analysis of immune responses of different hosts to Babesia divergens isolates from different geographic areas and capacity of culture-derived exoantigens to induce efficient cross-protection. Infection and Immunity 59, 27992805.
Schetters, T. ( 2005). Vaccination against canine babesiosis. Trends in Parasitology 21, 179184.
Schetters, T. ( 1995). Vaccine development from a commercial point of view. Veterinary Parasitology 57, 267275.
Schetters, T. P., Kleuskens, J. A., Scholtes, N. C., Gorenflot, A., Moubri, K. and Vermeulen, A. N. ( 2001). Vaccination of dogs against heterologous Babesia canis infection using antigens from culture supernatants. Veterinary Parasitology 100, 7586.
Schetters, T. P., Kleuskens, J. A., Scholtes, N. C., Pasman, J. W. and Bos, H. J. ( 1994). Vaccination of dogs against Babesia canis infection using antigens from culture supernatants with emphasis on clinical babesiosis. Veterinary Parasitology 52, 219233.
Schetters, T. P. and Montenero-James, S. ( 1995). Vaccines against babesiosis using soluble parasite antigens. Parasitology Today 11, 456462.
Timms, P., Dalgliesh, R. J., Barry, D. N., Dimmock, C. K. and Rodwell, B. J. ( 1983). Babesia bovis: comparison of culture-derived parasites, non-living antigen and conventional vaccine in the protection of cattle against heterologous challenge. Australian Veterinary Journal 60, 7577.
Valentin, A., Precigout, E., L'Hostis, M., Carcy, B., Gorenflot, A. and Schrevel, J. ( 1993). Cellular and humoral immune responses induced in cattle by vaccination with Babesia divergens culture-derived exoantigens correlate with protection. Infection and Immunity 61, 734741.
Yang, Y. S., Delbecq, S., Strub, M. P., Lohr, F., Schetters, T., Gorenflot, A., Precigout, E. and Roumestand, C. ( 2005). 1H, 15N and 13C Backbone resonance assignments of the 37 kDa surface antigen protein Bd37 from Babesia divergens. Journal of Biomolecular NMR 31, 6768.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords

Metrics

Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed