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Schistosoma mansoni: in vitro conversion of cercariae to schistosomula

  • L. K. Eveland and S. I. Morse
Extract

Schistosoma mansoni cercariae were incubated for 3–5 h at 37°C in various test solutions, and the rate and extent of conversion of th ecercariae to schistosomula were determined. Criteria used to identify schistosomula included: (1) loss of cercariai tail, (2) viability of organisms in saline but not in water, (3) pre-acetabular gland evacuation and (4) ability to survive in culture.

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Austin, F., Frappaola, P., Gilbert, B., Landis, W., da Rosa, M. N. & Stirewalt, M. (1974). Further studies of Schistosoma mansoni cercarial stimulation by crude egg lecithin and other lipids. Parasitology 64, 455–63.
Clegg, J. A. (1965). In vitro cultivation of Schistosoma mansoni. Experimental Parasitology 16, 133–47.
Clegg, J. A. & Smithers, S. R. (1972). The effect of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal of Parasitology 2, 7998.
Colley, D. G. & Wikel, S. K. (1974). Schistosoma mansoni: Simplified method for the production of schistosomules. Experimental Parasitology 35, 261–4.
Eveland, L. K. (1972). Schistosoma mansoni: Conversion of cercariae to schistosomula. Experimental Parasitology 32, 261–4.
Gazzinelli, G., de Oliveira, C. C., Figueiredo, E. A., Pereira, L. H., Coelho, P. M. Z. & Pellegrino, J. (1973). Schistosoma mansoni: Biochemical evidence for morphogenetic change from cercaria to schistosomule. Experimental Parasitology 34, 181–8.
Gilbert, B., da Rosa, M., Borojevic, R. & Pellegrino, J. (1972). Schistosoma mansoni: in vitro transformation of cercariae into schistosomula. Parasitology 64, 333–9.
Hirsch, J. G. & Fedorko, M. E. (1968). Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and ‘postfixation’ in uranyl acetate. Journal of Cell Biology 38, 615–27.
Lewert, R. M. & Para, J. B. (1966). The physiological incorporation of carbon-14 in Schistosoma mansoni cercariae. Journal of Infectious Diseases 116, 171–82.
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A., Figueiredo, E. A. & Pellegrino, J. (1974). Schistosoma mansoni: Defined system for stepwise transformation of cercariae to schistosomula in vitro. Experimental Parasitology 36, 360–72.
Stirewalt, M. A. (1966). Definition and collection in quantity of schistosomules of Schistosoma mansoni. Transactions of the Royal Society of Tropical Medicine and Hygiene 60, 352–60.
Stirewalt, M. A. & Uy, A. (1969). Schistosoma mansoni: Cercarial penetration and schistosomule collection in an in vitro system. Experimental Parasitology 26, 1728.
Tiba, Y., Holanda, J. C., Ramalho-Pinto, F. J., Gazzinelli, G. & Pellegrino, J. (1974). Schistosomula (Schistosoma mansoni) obtained in vitro: viability in culture and infectivity for mice. Transactions of the Royal Society of Tropical Medicine and Hygiene 68, 72.
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Parasitology
  • ISSN: 0031-1820
  • EISSN: 1469-8161
  • URL: /core/journals/parasitology
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