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Separation of Isospora (Toxoplasma) gondii cysts and cystozoites from mouse brain tissue by continuous density - gradient centrifugation

  • A. W. C. A. Cornelissen (a1), J. P. Overdulve (a1) and J. M. Hoenderboom (a1)
Summary

A simple, quick and reproducible method consisting of density-gradient centrifugation of homogenized infected mouse brain tissue on Percoll is described for the isolation and purification of cysts of Isospora (Toxoplasma) gondii. A 100% recovery of cysts, with 74·2% in a single fraction with a specific gravity of 1·056, was obtained by overlaying homogenates of infected mouse brains on a pre-formed Percoll gradient and centrifugation at low g forces. With this procedure recovery was independent of the age of the cysts. Titration of purified cystozoites showed there to be no loss of infectivity.

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References
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Dubey, J. P., Voller, A. & Frenkel, J. K. (1970). The Toxoplasma gondii oocyst from cat faeces. Journal of Experimental Medicine 132, 636–62.
Masihi, K. N. & Jíra, J.(1975). Studies on purification of Toxoplasma gondii zoites by sintered glass filters. Acta parasitologica Polonica 23, 653–8.
Masihi, K. N., Mach, O., Valkoun, A. & Jíra, J. (1976). Toxoplasma gondii: large scale purification by zonal density gradient centrifugation. Experimental Parasitology 39, 84–7.
Motomura, I. & Jo, K. (1970). Estimation of the number of parasites contained within a cyst, and growth and distribution of cysts in the brains of mice infected with Toxoplasma gondii. Tropical Medicine (Japan) 12, 41–50.
Nakabayashi, T. & Motomura, I. (1968). A method for separating cysts of Toxoplasma gondii from the infected mouse brains by multi-layer centrifugation with Gum Arabic solution. Tropical Medicine (Japan) 10, 72–80.
Overdulve, J. P. (1978). Excretion of Toxoplasma gondii by non-immunized and immunized cats; its role in the epidemiology of Toxoplasmosis. Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen, Series C 81, 1–18.
Overdulve, J. P. & Antonisse, H. W. (1970). Measurement of the effect of low temperature on Protozoa by titration. 1. A mathematical model for titration, using prepatent period or survival time; with a discussion of the method of the ID63. Experimental Parasitology 27, 310–22.
Pettersen, E. K. (1979). Destruction of Toxoplasma gondii by HCL solution. Acta pathologica microbiologica Scandinavica, Section B 87, 217–20.
Tryon, J. C., Weidner, E., Larson, A. D. & Hart, L. T. (1978). A rapid method for isolating purified Toxoplasma tachyzoites from peritoneal exudate and cell culture. Journal of Parasitology 64, 1127–9.
Van der Waay, D. (1959). Ontwikkeling en transmissie-mogelijkheden van Toxoplasma gondii in experimentele infectie met chronisch beloop bij muizen. Thesis, Leiden, pp. 4–8.
Work, K. (1971). Toxoplasmosis. With special reference to transmission and life cycle of Toxoplasma gondii. Acta pathologica microbiologica Scandinavica, Section B, Suppl. 221, 15–18.
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Parasitology
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