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The skin migratory stage of the schistosomulum of Schistosoma mansoni has a surface showing greater permeability and activity in membrane internalisation than other forms of skin or mechanical schistosomula

Published online by Cambridge University Press:  01 June 2015

WANDER DE JESUS JEREMIAS ,
JOSE RENAN DA CUNHA MELO ,
ELIO HIDEO BABA ,
PAULO MARCOS ZECH COELHO  and
JOHN ROBERT KUSEL
WANDER DE JESUS JEREMIAS
Affiliation:
Laboratory of schistosomiasis, Rene Rachou, Fiocuz, 1715 Augusto de Lima, Belo Horizonte, 30190-002 MG, Brazil
JOSE RENAN DA CUNHA MELO
Affiliation:
Depatarmento de Cirurgia, Faculdade de Medicina UFMG, Belo Horizonte, MG, Brazil
ELIO HIDEO BABA
Affiliation:
Laboratory of schistosomiasis, Rene Rachou, Fiocuz, 1715 Augusto de Lima, Belo Horizonte, 30190-002 MG, Brazil
PAULO MARCOS ZECH COELHO
Affiliation:
Laboratory of schistosomiasis, Rene Rachou, Fiocuz, 1715 Augusto de Lima, Belo Horizonte, 30190-002 MG, Brazil
JOHN ROBERT KUSEL*
Affiliation:
Centre for Open Studies, Glasgow University, Glasgow, UK
*
*Corresponding author. Centre for Open Studies, Glasgow University, Glasgow, UK. E-mail: john.kusel@glasgow.ac.uk
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Summary

Skin schistosomula can be prepared by collecting them after isolated mouse skin have been penetrated by cercariae in vitro. The schistosomula can also migrate out of isolated mouse skin penetrated by cercariae in vitro and from mouse skin penetrated by cercariae in vivo. Schistosomula can also be produced from cercariae applied through a syringe or in a vortex. When certain surface properties of the different forms of schistosomula were compared, those migrating from mouse skin penetrated by cercariae in vivo or in vitro had greatly increased permeability to membrane impermeant molecules such as Lucifer yellow and high molecular weight dextrans. These migrating forms also possessed surfaces which showed greatly enhanced uptake into internal membrane vesicles of the dye FM 143, a marker for endocytosis. This greatly enhanced activity and permeability of the surfaces of tissue migrating schistosomula is likely to be of great importance in the adaptation to the new host.

Keywords

Schistosomulasurface membranepermeabilityendocytosis
Type
Review Article
Information
Parasitology , Volume 142 , Issue 9 , August 2015 , pp. 1143 - 1151
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Copyright
Copyright © Cambridge University Press 2015 

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