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Studies on the nutrient uptake by the beet cyst nematode Heterodera schachtii by in situ microinjection of fluorescent probes into the feeding structures in Arabidopsis thaliana

  • A. Böckenhoff (a1) and F. M. W. Grundler (a1)

A method was developed, which enables substances to be injected into the feeding structure (syncytium) established by Heterodera schachtii in roots of Arabidopsis thaliana. The technique was used to study the uptake of nutrients by the feeding nematode. The fluorescent dye lucifer yellow CH (LYCH) and fluorescence-labelled dextrans of different molecular weights were injected into the thin and translucent roots of A. thaliana. Such roots are a feature of this plant and they provide optimal conditions for microinjection. Injected LYCH was taken up by feeding juveniles and adults, indicated by the staining of the alimentary duct and the digestive system. Fluorescent dextrans of 3, 10 and 20 kDa but not of 40 and 70 kDa were ingested, suggesting that molecules of a maximum Stokes radius of 3·2 to 4·4 nm are taken up. It is likely that the feeding tube, forming the interface between the plant cytosol and the nematode's digestive system, is responsible for this size exclusion effect. The injected fluorescent substances were not detected in plant cells adjacent to the syncytium or in the root vascular elements. Injections into parts of roots which were infested by several nematodes revealed that feeding H. schachtii individuals may share one syncytium.

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M. Betka , F. Crundler & U. Wyss (1991). Influence of changes in the nurse cell system (syncytium) on the development of the cyst nematode Heterodera schachtii: single amino acids. Phytopathology 81, 75–9.

M. A. Conkling , C. L. Cheng , Y. T. Yamamoto & H. M. Goodman (1990). Isolation of transcriptionally regulated root-specific genes from tobacco. Plant Physiology 93, 1203–11.

R. Dorhout , C. Kollüffel & F. J. Gommers (1988). Transport of an apoplastic fluorescent dye to feeding sites induced in tomato roots by Meloidogyne incognita. Phytopathology 78, 1421–4.

R. Dorhout , C. Kollüffel & F. J. Gommers (1992). Alteration of distribution of regions with high net proton extrusion in tomato roots infected with Meloidogyne incognita. Physiological and Molecular Plant Pathology 40, 153–62.

M. C. Erwee , P. B. Goodwin & A. J. E. Van Bel (1985). Cell—cell communications in the leaves of Commelina cyanea and other plants. Plant, Cell and Environment 8, 173–8.

O. G. Fisher (1988). Movement of lucifer yellow in leaves of Coleus blumei Benth. Plant. Cell and Environment 11, 639–44.

F. Grundler , M. Betka & U. Wyss (1991). Influence of changes in the nurse cell system (syncytium) on sex determination and development of the cyst nematode Heterodera schachtii: total amounts of proteins and amino acids. Phytopathology 81, 70–4.

M. G. K. Jones (1981). Host cell responses to endoparasitic nematode attack: structure and function of giant cells and syncytia. Annual and Applied Biology 97, 353–72.

H. J. Krauthausen & U. Wyss (1982). Influence of the cyst nematode Heterodera schachtii on relative changes in the pattern of free amino acids at the feeding sites. Physiological Plant Pathology 21, 425–36.

K. Luby-Phelps , D. L. Taylor & F. Lanni (1986). Probing the structure of cytoplasm. Journal of Cell Biology 102, 2015–22.

K. J. Oparka (1991). Uptake and compartment of fluorescent probes by plant cells. Journal of Experimental Botany 42, 565–79.

K. J. Oparka & D. A. M. Prior (1992). Direct evidence for pressure generated closure of plasmodesmata. The Plant Journal 2, 741–50.

B. A. Palevitz & P. K. Hepler (1985). Changes in dye coupling of stomatal cells of Allium and Commelina demonstrated by microinjection of Lucifer Yellow. Planta 164, 473–9.

B. Peters (1986). Fluorescence microphotolysis to measure nucleocytoplasmic transport and intracellular mobility. Biochimica et Biophysica Acta 864, 305–59.

H. J. Rumpenhorst (1984). Intracellular feeding tubes associated with sedentary plant parasitic nematodes. Nematologica 30, 7785.

P. Sijmons , F. M. W. Grundler , N. Von Mende , P. R. Burrows & U. Wyss (1991). Arabidopsis thaliana as a new model host for plant parasitic nematodes. The Plant Journal 1, 245–54.

N. Simionescu & G. E. Palade (1971). Dextrans and glycogens as particulate tracers for studying capillary permeability. Journal of Cell Biology 50, 616–24.

W. W. Stewart (1978). Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthylimide tracer. Cell 14, 741–59.

W. W. Stewart (1981). Lucifer dyes – highly fluorescent dyes for biological tracing. Nature, London292, 1721.

N. Thorball (1981). FITC-dextran tracers in microcirculatory and permeability studies using combined fluorescence stereo microscopy, fluorescence light microscopy and electron microscopy. Histochemistry 71, 209–33.

U. WYSS & F. M. W. Grundler (1992). Seminar: Heterodera schachtii and Arabidopsis thaliana, a model host–parasite interaction. Nematologica 38, 488–93.

U. Zimmermann , H. Räde & E. Steudle (1969). Kontinuierliche Druckmessung in Pflanzenzellen. Naturwissenschaften 56, 634.

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  • ISSN: 0031-1820
  • EISSN: 1469-8161
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