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    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.

    Frölich, Sonja and Wallach, Michael 2016. Use of fluorescent nanoparticles to investigate nutrient acquisition by developing Eimeria maxima macrogametocytes. Scientific Reports, Vol. 6, p. 29030.

    FRÖLICH, SONJA and WALLACH, MICHAEL 2015. F-actin distribution and function during sexual development in Eimeria maxima. Parasitology, Vol. 142, Issue. 07, p. 855.

    Wunderlich, Frank Al-Quraishy, Saleh Steinbrenner, Holger Sies, Helmut and Dkhil, Mohamed A. 2014. Towards identifying novel anti-Eimeria agents: trace elements, vitamins, and plant-based natural products. Parasitology Research, Vol. 113, Issue. 10, p. 3547.


The spatial organization and extraction of the wall-forming bodies of Eimeria maxima

  • DOI:
  • Published online: 14 March 2013

Eimeria maxima has been used as a model apicomplexan parasite to study sexual stage development and oocyst wall formation. A complete understanding of the wall's biochemical and biophysical properties is of great interest in research on all apicomplexan parasites. Purified gametocytes, zygotes and oocysts were analysed by three-dimensional confocal microscopy, and wide-field fluorescent microscopy was used to investigate the appearance and spatial organization of the 2 types of wall-forming bodies (WFBs). In addition, a variety of staining procedures and immunoassays were used to assess the biosynthesis, metabolic activity, intactness and molecular composition of the WFBs in situ. WFBs were extracted from gametocytes/zygotes and their composition was assessed by microscopy and SDS-PAGE analysis. It was concluded that isolated gametocytes are intact and metabolically active. Additionally, it was observed that the Type 1 WFBs are aligned at the periphery of the parasite and fuse together producing neutral lipid rich patches that appear to be inserted into the space between 2 parasite-specific membranes. Finally, it was shown that the WFBs extracted from purified gametocytes had the same shape, size and staining properties as those observed in situ, and contained the major glycoprotein antigens known to be present in these organelles.

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*Corresponding author: The iThree Institute, School of Medical and Molecular Biosciences, University of Technology Sydney, PO Box 123, Broadway, Sydney, New South Wales, 2007, Australia. Tel.: +61-2-9514-4082. Fax: +61-2-9514-4026. E-mail:
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