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To see or not to see: non-invasive imaging for improved readout of drug treatment trials in the murine model of secondary alveolar echinococcosis

Published online by Cambridge University Press:  08 March 2017

D. GORGAS ,
N. MARREROS ,
R. RUFENER ,
A. HEMPHILL  and
B. LUNDSTRÖM-STADELMANN Open the ORCID record for B. LUNDSTRÖM-STADELMANN [Opens in a new window]
D. GORGAS
Affiliation:
Division of Clinical Radiology, Vetsuisse-Faculty, Länggassstrasse 128, 3012 Berne, Switzerland
N. MARREROS
Affiliation:
Centre for Fish and Wildlife Health, Vetsuisse-Faculty, Länggassstrasse 122, 3012 Berne, Switzerland Vetsuisse-Faculty, Institute of Parasitology, Länggassstrasse 122, 3012 Berne, Switzerland
R. RUFENER
Affiliation:
Vetsuisse-Faculty, Institute of Parasitology, Länggassstrasse 122, 3012 Berne, Switzerland
A. HEMPHILL
Affiliation:
Vetsuisse-Faculty, Institute of Parasitology, Länggassstrasse 122, 3012 Berne, Switzerland
B. LUNDSTRÖM-STADELMANN*
Affiliation:
Vetsuisse-Faculty, Institute of Parasitology, Länggassstrasse 122, 3012 Berne, Switzerland
*
*Corresponding author. Britta Lundström-Stadelmann Institute of Parasitology, Länggassstrasse 122 3012 Bern, Switzerland. E-mail: Britta.lundstroem@vetsuisse.unibe.ch
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Summary

Alveolar echinococcosis (AE) is an emerging zoonotic disease caused by the cestode Echinococcus multilocularis. The secondary infection model of AE is based on intraperitoneal injection of disease-causing metacestodes into the peritoneal cavity of mice, which allows investigations on novel drugs or immunotherapeutical treatment options in vivo. So far, such in vivo studies assessed exclusively the parasite weight at the endpoint of a given treatment period. We here developed an ultrasound (US)-based scoring system that allows to follow-up parasite development in the living animal, and provides insights into parasite growth during the treatment phase. By this method a statistically significant difference between untreated and medicated mice with E. multilocularis infection was observed at 2 months post-infection, and the growth curve of the parasite load was described by a linear mixed model. High correlation and similar levels of variation were observed for the standard method based on parasite weight measurement, the novel US-based scoring system, as well volume segmentation by post-mortem magnetic resonance imaging. Thus, US-based scoring in the live animal has the potential to assist the 3R concept by contributing to the refinement and reduction of animal use in experimental echinococcosis.

Keywords

Echinococcus multilocularisultrasound scoringmagnetic resonance imaging3Rrefinement
Type
Research Article
Information
Parasitology , Volume 144 , Issue 7 , June 2017 , pp. 937 - 944
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Copyright
Copyright © Cambridge University Press 2017 

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