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Ultrastructural and molecular studies of Microgemma carolinus n. sp. (Microsporidia), a parasite of the fish Trachinotus carolinus (Carangidae) in Southern Brazil

Published online by Cambridge University Press:  19 July 2012

G. CASAL ,
E. MATOS ,
P. GARCIA ,
S. AL-QURAISHY  and
C. AZEVEDO
G. CASAL
Affiliation:
Laboratory of Pathology, Interdisciplinary Centre of Marine and Environmental Research, University of Porto (CIIMAR-UP), Rua dos Bragas, 177, 4050-123 Porto, Portugal Departmento de Ciências, Instituto Superior de Ciências da Saúde – Norte, CESPU, 4585-116 Gandra, Portugal Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, 4050-313 Porto, Portugal
E. MATOS
Affiliation:
Carlos Azevedo Research Laboratory, Federal Rural University of Amazonia, 66077-530 Belém, PA, Brazil
P. GARCIA
Affiliation:
Laboratory of Diagnostic and Pathology in Aquaculture, Federal University of Santa Catarina, 88040-970 Florianópolis, SC, Brazil
S. AL-QURAISHY
Affiliation:
Zoology Department, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia
C. AZEVEDO*
Affiliation:
Laboratory of Pathology, Interdisciplinary Centre of Marine and Environmental Research, University of Porto (CIIMAR-UP), Rua dos Bragas, 177, 4050-123 Porto, Portugal Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, 4050-313 Porto, Portugal
*
*Corresponding author: Department of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP), University of Porto, Rua de Jorge Viterbo Ferreira, no. 228, 4050-313 Porto, Portugal. Tel: +351 220 428 241. E-mail: azevedoc@icbas.up.pt
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Summary

A new species of Microsporidia Microgemma carolinus n. sp. found in the marine teleost Trachinotus carolinus collected in Florianópolis, Brazil was described based on light, ultrastructural and phylogenetic studies. This parasite developed in the liver forming whitish xenomas that contained different developmental stages with monokaryotic nuclei. The periphery of the xenoma presented some vacuolization and possessed several small projections in the membrane. The mature spores, measuring 3·8 ± 0·4 μm in length and 2·4 ± 0·4 μm in width, were slightly pyriform to ellipsoidal and had rounded ends. The polaroplast was bipartite and the isofilar polar filament was coiled with 8 – 9 turns in a single or double row at the posterior end of the spore. The nucleus was voluminous and in a central position, measuring ∼0·9 μm in diameter. A large posterior vacuole appeared as a pale area, occupying about a third of the spore length. The SSU rRNA gene was sequenced and analysed using maximum parsimony, maximum likelihood and neighbour-joining methods. This study allowed us to conclude that this was a new species of the genus Microgemma, being the first description of this genus from among South America fauna.

Keywords

fishparasiteMicrogemma carolinus n. sp.ultrastructurephylogeny
Type
Research Article
Information
Parasitology , Volume 139 , Issue 13 , November 2012 , pp. 1720 - 1728
Copyright
Copyright © Cambridge University Press 2012

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