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    Hill, S. M. and Crampton, J. M. 1994. DNA-based methods for the identification of insect vectors. Annals of Tropical Medicine & Parasitology, Vol. 88, Issue. 3, p. 227.
    van Vianen, Philip H. van Engen, Anneloes Thaithong, Sodsri van der Keur, Maarten Tanke, Hans J. van der Kaay, Hugo J. Mons, Barend and Janse, Chris J. 1993. Flow cytometric screening of blood samples for malaria parasites. Cytometry, Vol. 14, Issue. 3, p. 276.
    Hommel, Marcel 1991. 2. Impact of modern technologies on tropical medicine. Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 85, Issue. 2, p. 151.
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The use of biotin-labelled, synthetic DNA oligomers for the detection and identification of Plasmodium falciparum

  • M. A. Hughes (a1), M. Hommel (a2) and J. M. Crampton (a1)
Summary

An oligonucleotide mixture based on the 21 base pair repeat sequence of Plasmodium falciparum was covalently coupled to biotin and used as a probe to detect P. falciparum DNA. The limit of detection was 10 ng. This method was further developed as a fingerprint assay for parasite strain typing. After restrictian enzyme digestion, blotting and hybridization, distinct banding patterns were obtained for the strains tested and these were reproducible. In addition, discrete differences were found between PLF-3 S + /S-strains which may implicate genetic reorganization in the switching mechanism which occurs when parasites are passed from an intact to a splenectomized animal.

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COPYRIGHT: © Cambridge University Press 1990
References
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Parasitology
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