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The use of enzyme electrophoresis for the identification of the species of Eimeria present in field isolates of coccidia

  • H. D. Chapman (a1)

The electrophoretic mobility of glucose phosphate isomerase (GPI) has been used to identify Eimeria acervulina, E. praecox and E. mivati in isolates of coccidia from commercial sites in the United Kingdom. Mixtures of two or three species gave separate bands upon electrophoresis and each species could be identified provided that it formed at least 10–20% of the total number of oocysts present in a sample of 8 × 106 oocysts. The principal forms of GPI corresponded to the GPI of E. acervulina (H), E. praecox (H) and E. mivati (W) and were found in 38, 34 and 8 isolates respectively of the 46 examined. Three further variants of GPI are described.

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Chapman, H. D. (1982). The treatment of coccidiosis: studies on the sensitivity of recent field isolates of Eimeria acervulina type to anticoccidial drugs given in the drinking water. Journal of Comparative Pathology 92, 213–18.
Edgar, S. A. & Seibold, C. T. (1964). A new coccidium of chickens, Eimeria mivati sp.n (Protozoa: Eimeriidae) with details of its life history. Journal of Parasitology 50, 193204.
Horton-Smith, C. & Long, P. L. (1959). The effects of different anticoccidial agents on the intestinal coccidioses of the fowl. Journal of Comparative Pathology and Therapeutics 69, 192207.
Jeffers, T. K. (1974). Eimeria acervulina and E. maxima: Incidence and anticoccidial drug resistance of isolants in major broiler-producing areas. Avian Diseases 18, 331–42.
Joyner, L. P. & Long, P. L. (1974). The specific characters of the Eimeria, with special reference to the coccidia of the fowl. Avian Pathology 3, 145–57.
Long, P. L. (1967 a). Studies on Eimeria mivati in chickens and a comparison with Eimeria acervulina. Journal of Comparative Pathology 77, 315–25.
Long, P. L. (1967 b). Studies on Eimeria praecox. Johnson, 1930, in the chicken. Parasitology 57, 351–61.
Norton, C. C. & Joyner, L. P. (1980). Studies with Eimeria acervulina and E. mivati: pathogenicity and cross immunity. Parasitology 81, 315–23.
Reid, W. M., Womack, H. E. & Johnson, J. (1968). Coccidiosis susceptibility in layer flock replacement programs. Pountry Science 47, 892–9.
Shirley, M. W. (1975). Enzyme variation in Eimeria species of the chicken. Parasitology 71, 369–76.
Shirley, M. W. (1978). Electrophoretic variants ofphosphoglucomutase in different species and strains of Eimeria from the chicken. In Avian Coccidiosis (ed. Long, P. L., Boorman, K.N. and Freeman, B. M.), pp. 127–34. British Poultry Science Ltd.
Shirley, M. W. (1979): A reappraisal of the taxonomic status of Eimeria mivati Edgar and Seibold 1964, by enzyme electrophoresis and cross-immunity tests. Parasitology 78, 221–37.
Shirley, M. W., Millard, B. J. & Long, P. L. (1977). Studies on the growth, chemotherapy and enzyme variation of Eimeria acervulina var. diminuta and E. acervulina var. mivati. Parasitology 75, 165–82.
Shirley, M. W. & Rollinson, D. (1979). Coccidia: the recognition and characterization of populations of Eimeria. In Problems in the Identification of Parasites and their Vectors. Symposia of the British Society for Parasitology 17, 730.
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  • ISSN: 0031-1820
  • EISSN: 1469-8161
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