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Diversity of the W1 gene encoding flavonoid 3′,5′-hydroxylase in white- and purple-flowered soybeans

Published online by Cambridge University Press:  08 December 2014

Gyu Tae Park ,
Jagadeesh Sundaramoorthy ,
Jong-Beum Park ,
Jeong Dong Lee ,
Kwang Shik Choi ,
Jeong Hoe Kim ,
Hak Soo Seo ,
Soon-Ki Park  and
Jong Tae Song
Gyu Tae Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jagadeesh Sundaramoorthy
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jong-Beum Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jeong Dong Lee
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Kwang Shik Choi
Affiliation:
Department of Biology, Kyungpook National University, Daegu702-701, Republic of Korea
Jeong Hoe Kim
Affiliation:
Department of Biology, Kyungpook National University, Daegu702-701, Republic of Korea
Hak Soo Seo
Affiliation:
Department of Plant Bioscience, Seoul National University, Seoul151-742, Republic of Korea Bio-MAX Institute, Seoul National University, Seoul151-818, Republic of Korea
Soon-Ki Park
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
Jong Tae Song*
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu702-701, Republic of Korea
*
*Corresponding author. E-mail: jtsong68@knu.ac.kr
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Abstract

Cultivated soybeans [Glycinemax (L.) Merr.] have various flower colours such as dark purple, purple, light purple, pink, magenta, near white and white. About one-third of the soybean accessions in the United States Department of Agriculture – Germplasm Resource Information Network (USDA-GRIN) Soybean Germplasm Collections have white flowers and are the second dominant accessions after the purple-flowered accessions. Earlier studies have shown that the w1 recessive allele of the W1 gene encoding flavonoid 3′,5′-hydroxylase produces white flowers. In the present study, we aimed to understand why the white-flowered accessions have become abundant among the cultivated soybeans and what their genetic and regional origin is. For this purpose, 99 landraces with white flowers and 39 landraces with purple flowers from eight Asian countries and Russia were analysed with regard to the nucleotide sequences of the W1 locus. We not only found that the w1 alleles of the 99 white-flowered landraces were identical to those of the white-flowered Williams 82, but also found that these w1 alleles displayed no polymorphism at all. By carrying out a phylogenetic analysis, we were able to identify a group with W1 alleles from which the w1 allele might have diverged.

Keywords

phylogenetic analysispurple flowerssoybean landracesW1 genewhite flowers
Type
Research Article
Information
Plant Genetic Resources , Volume 13 , Issue 3 , November 2015 , pp. 213 - 218
Copyright
Copyright © NIAB 2014 

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