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Microsatellite high-resolution melting (SSR-HRM) analysis for identification of sweet cherry rootstocks in Greece

Published online by Cambridge University Press:  04 September 2013

Ganopoulos Ioannis
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki 54 124, Greece
Xanthopoulou Aliki
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki 54 124, Greece
Aravanopoulos Filippos
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece Laboratory of Forest Genetics and Tree Breeding, Faculty of Forestry and Natural Environment, Aristotle University of Thessaloniki, Thessaloniki, Greece
Kazantzis Konstantinos
Affiliation:
Pomology Institute, Hellenic Agricultural Organisation – ‘DEMETER’, PO Box 122, Naoussa 59200, Greece
Tsaftaris Athanasios
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece Department of Genetics and Plant Breeding, School of Agriculture, Aristotle University of Thessaloniki, Thessaloniki 54 124, Greece
Madesis Panagiotis
Affiliation:
Institute of Applied Biosciences, CERTH, Thermi, Thessaloniki 570 01, Greece
Corresponding
E-mail address:

Abstract

Sweet cherries can be grafted onto a wide range of rootstocks belonging to the genus Prunus. The identification of sweet cherry rootstocks using morphological traits is almost impossible particularly during the dormant season, yet it is very important for the grower to know exactly the rootstock as this has a major influence on cultivar performance and agricultural practices. However, DNA-based molecular analysis carried out on actively growing shoot tips, leaves or dormant buds provides a good opportunity to reliably distinguish the rootstocks. Herein, we have integrated high-resolution melting (HRM) analysis, coupled with five simple sequence repeat (SSR) markers, in order to facilitate the identification of the most popular sweet cherry rootstocks in Greece. The five SSR loci used were highly informative and generated a unique melting curve profile of microsatellites for each of the six sweet cherry rootstocks tested. In particular, one marker, BPPCT002, with six HRM profiles was sufficient to discriminate all the sweet cherry rootstocks studied, highlighting its potential use for rootstock identification. Hence, this assay provided a flexible, cost-effective and closed-tube microsatellite genotyping method well suited to sweet cherry rootstock identification.

Type
Short Communications
Copyright
Copyright © NIAB 2013 

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References

Chatzicharisis, I and Kazantzis, K (2011) Prunus avium rootstocks. In: Chatzicharisis I (ed.) Monography. Naousa, Greece: Institute of Pomology, pp. 4–27.Google Scholar
Ganopoulos, I, Argiriou, A and Tsaftaris, A (2011) Microsatellite high resolution melting (SSR-HRM) analysis for authenticity testing of protected designation of origin (PDO) sweet cherry products. Food Control 22: 532541.CrossRefGoogle Scholar
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Rom, RC (1982) A new philosophy for peach rootstock development. Fruit Varieties Journal 36: 3436.Google Scholar
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Microsatellite high-resolution melting (SSR-HRM) analysis for identification of sweet cherry rootstocks in Greece
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