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An Irish perennial ryegrass genetic resource collection clearly divides into two major gene pools

  • Susanne Barth (a1), Sarah Katherine McGrath (a1) (a2), Sai Krishna Arojju (a1) (a2) and Trevor Roland Hodkinson (a2)

This study assessed the genetic diversity in 928 individuals from 40 diploid populations of Lolium perenne using nuclear simple sequence repeat markers, including 22 accessions of Irish ecotypes, seven European ecotypes and 11 released varieties. High levels of allelic and genetic diversity were determined, with intra-population variation accounting for the majority of the variation. The majority of the accessions deviated from Hardy–Weinberg equilibrium and had relatively high inbreeding coefficients. Two major gene pools of ecotypic accessions were defined by unweighted pair group method with arithmetic mean (UPGMA) and PCA analyses. One of these two gene pools accounted for two-thirds of the ecotypes and included most of the current Irish and Northern Irish breeding materials and about half of the European ecotypes included in this study; these European ecotypes performed well under Irish selection conditions. Population structure and differentiation analyses using Structure analysis and analysis of molecular variance confirmed the results found in the UPGMA and PCA analyses. These results will be useful for breeders who wish to exploit specific pools from ecotype collections.

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J Auzanneau , C Huyghe , B Julier and P Barre (2007) Linkage disequilibrium in synthetic varieties of perennial ryegrass. Theoretical and Applied Genetics 115: 837847.

EE Berg and JL Hamrick (1995) Fine-scale genetic structure of a Turkey oak forest. Evolution 49: 110120.

T Blackmore , I Thomas , R McMahon , W Powell and M Hegarty (2015) Genetic–geographic correlation revealed across a broad European ecotypic sample of perennial ryegrass (Lolium perenne) using array-based SNP genotyping. Theoretical and Applied Genetics 128: 19171932.

S Bolaric , S Barth , AE Melchinger and UK Posselt (2005b) Molecular genetic diversity within and among German ecotypes in comparison to European perennial ryegrass cultivars. Plant Breeding 124: 257262.

D Charlesworth and SI Wright (2001) Breeding systems and genome evolution. Current Opinion in Genetics and Development 11: 685690.

A Cresswell , NRS Hamilton , AK Roy and BMF Viegas (2001) Use of amplified fragment length polymorphism markers to assess genetic diversity of Lolium species from Portugal. Molecular Ecology 10: 229241.

D Earl and B vonHoldt (2012) STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method. Conservation Genetics Resources 4: 359361.

G Evanno , S Regnaut and J Goudet (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology 14: 26112620.

D Falush , M Stephens and JK Pritchard (2007) Inference of population structure using multilocus genotype data: dominant markers and null alleles. Molecular Ecology 7: 574578.

GP Gill , PL Wilcox , DJ Whittaker , RA Winz , P Bickerstaff , CE Echt , J Kent , MO Humphreys , KM Elborough and RC Gardner (2006) A framework linkage map of perennial ryegrass based on SSR markers. Genome 49: 354364.

S Guo and E Thompson (1992) Performing the exact test of Hardy–Weinberg proportion for multiple alleles. Biometrics 48: 361371.

KM Guthridge , MP Dupal , R Kolliker , ES Jones , KF Smith and JW Forster (2001) AFLP analysis of genetic diversity within and between populations of perennial ryegrass (Lolium perenne L.). Euphytica 122: 191201.

M Jakobsson and NA Rosenberg (2007) CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics 23: 18011806.

ES Jones , MP Dupal , R Kolliker , MC Drayton and JW Forster (2001) Development and characterisation of simple sequence repeat (SSR) markers for perennial ryegrass. Theoretical and Applied Genetics 102: 405415.

R Kolliker , FJ Stadelmann , B Reidy and J Nosberger (1999) Genetic variability of forage grass cultivars: a comparison of Festuca pratensis Huds., Lolium perenne L., and Dactylis glomerata L. Euphytica 106: 261270.

C Kubik , M Sawkins , WA Meyer and BS Gaut (2001) Genetic diversity in seven perennial ryegrass (Lolium perenne L.) cultivars based on SSR markers. Crop Science 41: 15651572.

P Lem and J Lallemand (2003) Grass consensus STS markers: an efficient approach for detecting polymorphism in Lolium . Theoretical and Applied Genetics 107: 11131122.

S McGrath , TR Hodkinson and S Barth (2007) Extremely high cytoplasmic diversity in natural and breeding populations of Lolium (Poaceae). Heredity (Edinburgh) 99: 531544.

S McGrath , TR Hodkinson , TM Charles , DG Zen and S Barth (2010) Variation in inflorescence characters and inflorescence development in ecotypes and cultivars of Lolium perenne L. Grass and Forage Science 65: 398409.

A Momotaz , JW Forster and T Yamada (2004) Identification of cultivars and accessions of Lolium, Festuca and Festulolium hybrids through the detection of simple sequence repeat polymorphism. Plant Breeding 123: 370376.

M Nei (1972) Genetic distance between populations. The American Naturalist 106: 283292.

M Nei (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70: 33213323.

RC Ponting , MC Drayton , NOI Cogan , MP Dobrowolski , GC Spangenberg , KF Smith and JW Forster (2007) SNP discovery, validation, haplotype structure and linkage disequilibrium in full-length herbage nutritive quality genes of perennial ryegrass (Lolium perenne L.). Molecular Genetics and Genomics 278: 585597.

JK Pritchard , M Stephens , NA Rosenberg and P Donnelly (2000 a) Association mapping in structured populations. American Journal of Human Genetics 67: 170181.

RK Ramasamy , S Ramasamy , BB Bindroo and VG Naik (2014) STRUCTURE PLOT: a program for drawing elegant STRUCTURE bar plots in user friendly interface. SpringerPlus 3: 431.

I Roldan-Ruiz , FA van Eeuwijk , TJ Gilliland , P Dubreuil , C Dillmann , J Lallemand , M De Loose and CP Baril (2001) A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties. Theoretical and Applied Genetics 103: 11381150.

NA Rosenberg , JK Pritchard , J Weber , HM Cann , KK Kidd , LA Zhivotovsky and MW Feldman (2002) Genetic structure of human populations. Science 298: 23812385.

N Salamin , TR Hodkinson and V Savolainen (2005) Towards building the tree of life: a simulation study for all angiosperm genera. Systematic Biology 54: 183196.

L Skøt , NRS Hamilton , S Mizen , KH Chorlton and ID Thomas (2002) Molecular genecology of temperature response in Lolium perenne: 2. Association of AFLP markers with ecogeography. Molecular Ecology 11: 18651876.

L Skøt , MO Humphreys , I Armstead , S Heywood , KP Skøt , R Sanderson , ID Thomas , KH Chorlton and NRS Hamilton (2005) An association mapping approach to identify flowering time genes in natural populations of Lolium perenne (L.). Molecular Breeding 15: 233245.

B Studer , F Widmer , J Enkerli and R Kolliker (2006) Development of novel microsatellite markers for the grassland species Lolium multiflorum, Lolium perenne and Festuca pratensis . Molecular Ecology Notes 6: 11081110.

LB Turner , AJ Cairns , IP Armstead , J Ashton , K Skøt , D Whittaker and MO Humphreys (2006) Dissecting the regulation of fructan metabolism in perennial ryegrass (Lolium perenne) with quantitative trait locus mapping. New Phytologist 169: 4557.

R Van Treuren , N Bas , PJ Goossens , J Jansen and JM Van Soest (2005) Genetic diversity in perennial ryegrass and white clover among old Dutch grasslands as compared to cultivars and nature reserves. Molecular Ecology 14: 3952.

SE Warnke , RE Barker , G Jung , SC Sim , MA Rouf Mian , MC Saha , LA Brilman , MP Dupal and JW Forster (2004) Genetic linkage mapping of an annual × perennial ryegrass population. Theoretical and Applied Genetics 109: 294304.

BS Weir and CC Cockerham (1984) Estimating F-statistics for the analysis of population structure. Evolution 38: 13581370.

Y Xing , U Frei , B Schejbel , T Asp and T Lubberstedt (2007) Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in Lolium perenne . BMC Plant Biology 7: 43.

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