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Association mapping of genomic loci linked with Fusarium wilt resistance (Foc2) in chickpea

Published online by Cambridge University Press:  21 April 2021

Uday Chand Jha Open the ORCID record for Uday Chand Jha [Opens in a new window] ,
Rintu Jha ,
Abhishek Bohra ,
Lakshmaiah Manjunatha ,
Parasappa Rajappa Saabale ,
Swarup K. Parida ,
Sushil Kumar Chaturvedi ,
Virevol Thakro  and
Narendra Pratap Singh
Uday Chand Jha*
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
Rintu Jha
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
Abhishek Bohra
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
Lakshmaiah Manjunatha
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
Parasappa Rajappa Saabale
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
Swarup K. Parida
Affiliation:
National Institute of Plant Genome Research (NIPGR), New Delhi, India
Sushil Kumar Chaturvedi
Affiliation:
Rani Lakshmi Bai Central Agricultural University, Jhansi284 003, India
Virevol Thakro
Affiliation:
National Institute of Plant Genome Research (NIPGR), New Delhi, India
Narendra Pratap Singh
Affiliation:
ICAR-Indian Institute of Pulses Research (IIPR), Kanpur208024, India
*
*Corresponding author. E-mail: uday_gene@yahoo.co.in
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Abstract

Improving plant resistance against Fusarium wilt (FW) is key to sustaining chickpea production worldwide. Given this, the current study tested a set of 75 FW-responsive chickpea breeding lines including checks in a wilt-sick plot for two consecutive years (2016 and 2017). Genetic diversity analysis using 75 simple sequence repeats (SSRs) revealed a total of 267 alleles with an average of 3.56 alleles per marker. The entire set was divided into two major classes based on clustering method and factorial analysis. Similarly, STRUCTURE analysis placed the 75 genotypes into three distinct sub-groups (K = 3). Marker-trait association (MTA) analysis using the generalized linear model approach revealed nine and eight significant MTAs for FW resistance in the years 2016 and 2017, respectively. Three significant MTAs were obtained for FW resistance following the mixed linear model approach for both years. The SSR markers CESSR433, NCPGR21 and ICCM0284 could be potentially employed for targeted and accelerated improvement of FW resistance in chickpea. To the best of our knowledge, this is the first report on association mapping of the genomic loci controlling FW (Foc2) resistance in chickpea.

Keywords

association mappingCicer arietinumFusarium wiltSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 3 , June 2021 , pp. 195 - 202
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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