Skip to main content

Development and application of EST-SSRs for diversity analysis in Ethiopian grass pea

  • M. Ponnaiah (a1), E. Shiferaw (a1), M. E. Pè (a1) and E. Porceddu (a2)

To date, very limited molecular knowledge and molecular tools are available on grass pea (Lathyrus sativus L.). Herein, we present a genetic diversity analysis of 20 grass pea accessions collected from various regions of Ethiopia by means of seven newly developed Lathyrus expressed sequence tag-derived simple sequence repeat (EST-SSR) markers and four cross-transferable EST-SSRs derived from Medicago truncatula L. Forty-five alleles were detected among all the analyzed accessions. The number of alleles/locus ranged from two to seven with an average of four alleles/locus. The observed heterozygosity (Ho) ranged from 0.320 to 0.504, while the expected heterozygosity (He) ranged from 0.354 to 0.470. FST values estimated by analysis of molecular variance were 0.01, 0.15 and 0.84 for among regions, among accessions and within accessions, respectively, indicating that most of the variation (84%) resides within accession. The model-based cluster analysis grouped the accessions into three clusters grouping accessions, irrespective of their collection regions.

Corresponding author
*Corresponding author. E-mail:
Hide All
CSA(2007) Agricultural sample survey 2006/2007. Report on area and production of crops volume I. Ethiopia: Central Statistics Authority.
Campbell, CG (1997) Grass pea. Lathyrus sativus L. Promoting the conservation and use of underutilized and neglected crops. 18. Rome: Institute of Plant Genetics and Crop Plant Research, Gatersleben/International Plant Genetic Resources Institute.
Chowdhury, MA and Slinkard, AE (1997) Natural outcrossing in grasspea. J Hered 88: 154156.
Chowdhury, MA and Slinkard, AE (2000) Genetic diversity in grass pea (Lathyrus sativus L.). Genetic Resources and Crop Evolution 47: 163169.
Ellis, JR and Burke, JM (2007) EST-SSRs as a resource for population genetic analyses. Heredity 99: 125132.
Eujayl, I, Sledge, MK, Wang, L, May, GD, Chekhovskiy, K, Zwonitzer, JC and Mian, MAR (2004) Medicago truncatula EST-SSRs reveal cross-species genetic markers for Medicago spp. Theoretical and Applied Genetics 108: 414422.
Falush, D, Stephens, M and Pritchard, JK (2003) Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164: 15671587.
Gupta, PK, Rustgi, S, Sharma, S, Singh, R, Kumar, N and Balyan, HS (2003) Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat. Molecular Genetics and Genomics 270: 315323.
Gutiérrez-Marcos, JF, Vaquero, F, Sáenz de Miera, LE and Vences, FJ (2006) High genetic diversity in a world-wide collection of Lathyrus sativus L. revealed by isozymatic analysis. Plant Genetic Resources 4: 159171.
Gutierrez, MV, Vaz Patto, MC, Huguet, T, Cubero, JI, Moreno, MT and Torres, AM (2005) Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops. Theoretical and Applied Genetics 110: 12101217.
Holton, TA, Christopher, JT, McClure, L, Harker, N and Henry, RJ (2002) Identification and mapping of polymorphic SSR markers from expressed gene sequences of barley and wheat. Molecular Breeding 9: 6371.
Kantety, R, La Rota, VM, Matthews, DE and Sorrells, ME (2002) Data mining for simple sequence repeats in expressed sequence tags from barley, maize, rice, sorghum and wheat. Plant and Molecular Biology 48: 501510.
Katti, MV, Ranjekar, PK and Gupta, VS (2001) Differential distribution of simple sequence repeats in eukaryotic genome sequences. Molecular Biology and Evolution 18: 11611167.
Liu, K and Muse, SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21: 21282129.
Louette, D, Charrier, A and Berthaud, J (1997) In situ conservation of maize in Mexico: genetic diversity and maize seed management in a traditional community. Economic Botany 51: 2038.
Peakall, R and Smouse, PE (2006) GENALEX6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288295.
Pritchard, JK, Stephens, M and Donnelly, P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945959.
Schuelke, M (2000) An economic method for the fluorescent labeling of PCR fragments. Nature Biotechnology 18: 233234.
Tadesse, W and Bekele, E (2003) Phenotypic diversity of Ethiopian grass pea (Lathyrus sativus L.) in relation to geographical regions and altitudinal range. Genetic Resources and Crop Evolution 50: 497505.
Van Oosterhout, C, Hutchinson, WF, Wills, DPM and Shipley, P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Molecular Ecology Notes 4: 535538.
Yadav, SS and Bejiga, G (2006) In: Brink, M and Belay, G (eds) Lathyrus sativus L. Record from Protabase. Wageningen: PROTA (Plant Resources of Tropical Africa),
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
  • URL: /core/journals/plant-genetic-resources
Please enter your name
Please enter a valid email address
Who would you like to send this to? *


Type Description Title
Supplementary materials

Ponnaiah Supplementary Material
Ponnaiah Supplementary Material

 Word (33 KB)
33 KB


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed