Skip to main content
×
Home
    • Aa
    • Aa

Direct multiplex PCR for grapevine genotyping and varietal identification

  • Daniele Migliaro (a1), Giacomo Morreale (a1), Massimo Gardiman (a1), Sara Landolfo (a1) and Manna Crespan (a1)...
Abstract

Grapevine cultivar identification is based mainly on two complementary methodologies: microsatellite (simple sequence repeat (SSR)) DNA analysis and traditional ampelography. Here, we report a direct multiplex PCR approach that allows the simultaneous amplification of 11 SSR loci from crude samples, i.e. bypassing DNA extraction, by using an engineered DNA polymerase improved to tolerate plant PCR inhibitors. Many different plant tissues were successfully amplified: leaf, root, wood, berry flesh and skin, stalk and must, from wine and table grape varieties, and rootstocks. The direct multiplex PCR that we propose is quicker and cheaper than the methodologies used until now, and provides accurate results. Thus, SSR DNA analysis becomes economically more accessible to a larger number of potential users in addition to research institutes.

Copyright
Corresponding author
*Corresponding author: E-mail: manna.crespan@entecra.it
Linked references
Hide All

This list contains references from the content that can be linked to their source. For a full set of references and notes please see the PDF or HTML where available.

ST Kalinowski , ML Taper and TC Marshall (2007) Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Molecular Ecology 16: 10991106.

D Merdinoglu , G Butterlin , L Bevilacqua , V Chiquet , AF Adam-Blondon and S Decroocq (2005) Development and characterization of a large set of microsatellite markers in grapevine (Vitis vinifera L.) suitable for multiplex PCR. Molecular Breeding 15: 349366.

P Moreno-Sanz , MD Loureiro and B Suárez (2011) Microsatellite characterization of grapevine (Vitis vinifera L.) genetic diversity in Asturias (Northern Spain). Scientia Horticulturae 129: 433440.

P This , A Jung , P Boccacci , J Borrego , R Botta , L Costantini , M Crespan , GS Dangl , C Eisenheld , F Ferreira-Monteiro , S Grando , J Ibáñez , T Lacombe , V Laucou , M Magalhães , CP Meredith , N Milani , E Peterlunger , F Regner , L Zulini and E Maul (2004) Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics 109: 14481458.

P This , T Lacombe and MR Thomas (2006) Historical origins and genetic diversity of wine grapes. Trends in Genetics 22: 511519.

LJ Welter , N Göktürk-Baydar , M Akkurt , E Maul , R Eibach , R Töpfer and EM Zyprian (2007) Genetic mapping and localization of quantitative trait loci affecting fungal disease resistance and leaf morphology in grapevine (Vitis vinifera L.). Molecular Breeding 20: 359374.

Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Plant Genetic Resources
  • ISSN: 1479-2621
  • EISSN: 1479-263X
  • URL: /core/journals/plant-genetic-resources
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords:

Type Description Title
WORD
Supplementary Materials

Migliaro Supplementary Material
Tables 1-2

 Word (53 KB)
53 KB