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Diversity and genetic structure of cassava landraces and their wild relatives (Manihot spp.) in Colombia revealed by simple sequence repeats

  • E. Tovar (a1), J. L. Bocanegra (a1), C. Villafañe (a2), L. Fory (a3), A. Velásquez (a3), G. Gallego (a3) and R. Moreno (a1)...

Understanding the genetic composition and population structure of plant species at a molecular level is essential for the development of adequate strategies aimed at enhancing the conservation and use of their genetic resources. In addition, such knowledge can help to plan ahead for a scenario under which wild and cultivated species come into contact with their genetically modified (GM) counterpart(s). Using ten simple sequence repeat markers, we genotyped 409 samples pertaining to the species in the Manihot genus known to occur in Colombia, i.e. cassava (Manihot esculenta) and its wild relatives Manihot brachyloba, Manihot carthaginensis and Manihot tristis. High genetic variation was observed in all the species (HE= 0.212–0.603), with cassava showing highest diversity. Most of the genetic variation was found within species populations. Our results suggest that outcrossing events among populations occur much more frequently in M. tristis and M. esculenta, and particularly so in the latter, where the exchange of varieties among local farmers plays a key role in maintaining and introducing new genetic diversity. The occurrence of gene flow within and among populations of Manihot species in Colombia becomes relevant in a biosafety context, where gene flow from GM cassava, if introduced to the country, might have detrimental effects on the structure and dynamics of populations of wild species. The baseline information on the genetic diversity and structure of the four Colombian species that we have presented here provides a first and indispensable step towards the development of targeted interventions necessary to preserve their genetic resources.

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ACAllem (1994) The origin of Manihot esculenta Crantz (Euphorbiaceae). Genetic Resources and Crop Evolution 41: 133150.

ACAllem , RAMendes , ANSalomão and MLBurle (2001) The primary gene pool of cassava (Manihot esculenta Crantz subspecies esculenta, Euphorbiaceae). Euphytica 120: 127132.

ADuputié , PDavid , CDebain and DMcKey (2007) Natural hybridization between a clonally propagated crop, cassava (Manihot esculenta ssp. esculenta) and a wild relative in French Guiana. Molecular Ecology 16: 30253038.

MElias , OPanaud and TRobert (2000) Assessment of genetic variability in a traditional cassava (Manihot esculenta Crantz) farming system, using AFLP markers. Heredity 85: 219230.

MElias , LPenet , PVindry , DMcKey , OPanaud and TRobert (2001) Unmanaged sexual reproduction and the dynamics of genetic diversity of a vegetatively propagated crop plant, cassava (Manihot esculenta Crantz), in a traditional farming system. Molecular Ecology 10: 18951907.

MElias , GSantos Mühlen , DMcKey , ACRoa and JTohme (2004) Genetic diversity of traditional south american landraces of cassava (Manihot esculenta Crantz): an analysis using microsatellites. Economic Botany 58: 242256.

NCEllstrand and DRElam (1993) Population genetic consequences of small population size: implications for plant conservation. Annual Review of Ecology, Evolution, and Systematics 24: 217242.

LExcoffier and HELLischer (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10: 564567.

MFregene , MSuarez , JMkumbira , HKulembeka , ENdedya , AKulaya , SMitchel , UGullberg , HRosling , ADixon and SKresovich (2003) Simple sequence repeat (SSR) diversity of cassava (Manihot esculenta Crantz) landraces: genetic diversity and differentiation in a predominantly asexually propagated crop. Theoretical and Applied Genetics 107: 10831093.

UIhemere , DArias-Garzon , SLawrence and RTSayre (2006) Genetic modification of cassava for enhanced starch production. Plant Biotechnology Journal 4: 453465.

RSKawuki , MFerguson , MLabuschagne , LHerselman and DJKim (2009) Identification, characterization and application of single nucleotide polymorphisms for diversity assessment in cassava (Manihot esculenta Crantz). Molecular Breeding 23: 669684.

LAKelley , SPGardner and MJSutcliffe (1996) An automated approach for clustering an ensemble of NMR-derived protein structures into conformationally-related subfamilies. Protein Engineering 9: 10631065.

FALefevre and SCharrier (1993) Isozyme diversity within African Manihot germplasm. Euphytica 66: 7380.

RECMba , PStephenson , KEdwards , SMelzer , JMkumbira , UGullberg , KApel , MGale , JTohme and MFregene (2001) Simple sequence repeat (SSR) markers survey of the cassava (Manihot esculenta Crantz) genome: towards an SSR-based molecular genetic map of cassava. Theoretical and Applied Genetics 102: 2231.

RMeireles da Silva , GBandel and PSMartins (2003) Mating system in an experimental garden composed of cassava (Manihot esculenta Crantz) ethnovarieties. Euphytica 134: 127135.

JAMontagnac , CRDavis and SATanumihardio (2009) Nutritional value of cassava for use as a staple food and recent advances for improvement. Comprehensive Reviews in Food Science and Food Safety 8: 181194.

MMontero-Rojas , AMCorrea and DSiritunga (2011) Molecular differentiation and diversity of cassava (Manihot esculenta) taken from 162 locations across Puerto Rico and assessed with microsatellite markers. AoB Plants 2011: plr010. doi:10.1093/aobpla/plr010.

KMOlsen (2004) SNPs, SSRs and inferences on cassava's origin. Plant Molecular Biology 56: 517526.

KMOlsen and BASchaal (1999) Evidence on the origin of cassava: phylogeography of Manihot esculenta . Proceedings of the National Academy of Sciences of the United States of America 96: 55865591.

BPujol , PDavid and DMcKey (2005) Microevolution in agricultural environments: how a traditional Amerindian farming practice favours heterozygosity in cassava (Manihot esculenta Crantz. Euphorbiaceae). Ecology Letters 8: 138147.

AAJRaji , JVAnderson , OAKolade and CDUgwu (2009 a) Gene-based microsatellites for cassava (Manihot esculenta Crantz): prevalence, polymorphisms, and cross-taxa utility. BMC Plant Biology 9: 118.

AAJRaji , IFawole , MGedil and AGODixon (2009 b) Genetic differentiation analysis of African cassava (Manihot esculenta) landraces and elite germplasm using amplified fragment length polymorphism and simple sequence repeat markers. Annals of Applied Biology 155: 187199.

ACRoa , MMMaya , MCDuque , JTohme , ACAllem and MWBonierbale (1997) AFLP analysis of relationships among cassava and other Manihot species. Theoretical and Applied Genetics 95: 741750.

JSalick , NCellinese and SKnapp (1997) Indigenous diversity of cassava: generation, maintenance, use and loss among the Amuesha, Peruvian upper Amazon. Economic Botany 51: 619.

JBMSambatti , PSMartins and AAndo (2001) Folk taxonomy and evolutionary dynamics of cassava: a case study in Ubatuba. Brazil. Economic Botany 55: 93105.

RSayre , JRBeeching , EBCahoon , CEgesi , CFauquet , JFellman , MFregene , WGruissem , SMallowa , MManary , BMaziya-Dixon , AMbanaso , DPSchachtman , DSiritunga , NTaylor , HVanderschuren and PZhang (2011) The bio-cassava plus program: biofortification of cassava for sub-saharan Africa. Annual Review of Plant Biology 62: 251272.

BASchaal , DAHayworth , KMOlsen , JTRauscher and WASmith (1998) Phylogeographic studies in plants: problems and prospects. Molecular Ecology 7: 465474.

MVBMSiqueira , JRQueiroz-Silva , EABressan , ABorges , KJCPereira , JGPinto and EAVeasey (2009) Genetic characterization of cassava (Manihot esculenta) landraces in Brazil assessed with simple sequence repeats. Genetics and Molecular Biology 32: 104110.

MSlatkin and NHBarton (1989) A comparison of three indirect methods for estimating average levels of gene flow. Evolution 43: 13491368.

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