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Genetic diversity among Lagenaria siceraria accessions containing resistance to root-knot nematodes, whiteflies, ZYMV or powdery mildew

  • Amnon Levi (a1), Judy Thies (a1), Kai-shu Ling (a1), Alvin M. Simmons (a1), Chandrasekar Kousik (a1) and Richard Hassell (a2)...

In recent years, there has been an increased interest in Europe and in the USA in grafting watermelon onto bottle gourd, Lagenaria siceraria (Mol.) Standl. In this study, genetic diversity and relationships were examined [using 236 sequence-related amplified polymorphism markers] among 56 United States plant introductions (PIs) of L. siceraria and PIs of important cucurbit crops [including Cucurbita maxima Duchesne (winter squash), Cucurbita pepo L. (squash and pumpkin), Citrullus spp. (watermelon), Cucumis melo L. (melon) and Cucumis sativus L. (cucumber)]. The analysis showed that L. siceraria is distinct and has similar genetic distances to the cucurbit species examined herein. The L. siceraria PIs were assembled into two major clusters. One cluster includes groups of PIs collected mostly in South Asia (India) and a few PIs collected in the Mediterranean region and in Northeast Africa. The second cluster includes groups of PIs collected mainly in Southern Africa and in North, Central and South America, and PIs collected in China, Indonesia and Cyprus. All L. siceraria PIs in this study were susceptible to the southern root-knot nematode (RKN) [Meloidogyne incognita (Kofoid and White) Sandground]. However, several PIs, among them a group of closely related PIs collected in Mexico and Florida, were less infected with southern RKNs. All L. siceraria PIs were infested with whiteflies [Bemisia tabaci (Gennadius)], while several PIs were less infested than others and need further evaluation and selection for developing breeding lines that may be less appealing to this pest. Most of the PIs that showed resistance to zucchini yellow mosaic virus and tolerance to powdery mildew were collected in India and belong to the same phylogenetic groups (PGs). Experiments with L. siceraria PIs representing different PGs showed similar grafting compatibility with watermelon. Findings from this study should be useful for the development of superior L. siceraria rootstock lines with enhanced resistance to diseases and insect pests of cucurbit crops.

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Amaido A (2004) Alternatives of methyl bromide adopted for cucurbit production in projects funded by montreal protocol. In: Batchelor T and Alfarroba F (eds) Proceedings of the Fifth International Conference on Alternatives to Methyl Bromide, Lisbon, pp. 7174.
Cohen R, Burger Y, Horev C, Koren A and Edelstein M (2007) Introducing grafted cucurbits to modern agriculture: the Israeli experience. Plant Disease 91: 916923.
Colla G, Fanasca S, Cardarelli M, Rouphael Y, Saccardo F, Graifenberg A and Curadi M (2005) Evaluation of salt tolerance in rootstocks of Cucurbitaceae. Acta Horticulturae 697: 469474.
Cutler HC and Whitaker TW (1967) Cucurbits from the Techuacan Caves. In: Byers DS (ed.) The Prehistory of the Tehuacan Valley: Environment and Subsistence. 1. Austin, TX/London: University of Texas Press, pp. 212219.
Davis AR, Perkins-Veazie P, Sakata Y, López-Galarza S, Maroto JV, Lee SG, Huh YC, Sun Z, Miguel A, King SR, Cohen R and Jung-Myung L (2008) Cucurbit grafting. Critical Reviews in Plant Sciences 27: 5074.
Decker-Walters D, Staub JE, Chung SM and Nakata E (2001) Diversity in landraces and cultivars of bottle gourd (Lagenaria siceraria: Cucurbitaceae) as assessed by random amplified polymorphic DNA. Genetic Resources and Crop Evolution 48: 369380.
Decker-Walters DS, Wilkins-Ellert M, Chung SM and Staub JE (2004) Discovery and genetic assessment of wild bottle gourd [Lagenaria siceraria (Mol.) Standley; Cucurbitaceae] from Zimbabwe. Economic Botany 58: 501508.
Edelstein M, Tadmore Y, Abo-Moch F, Karchi Z and Mansur F (2000) The potential of Lagenaria rootstock to confer resistance to the carmine spider mite. Entomology Research 90: 113117.
Erickson DL, Smith BD, Clarke AC, Sandweiss DH and Tuross N (2005) An Asian origin for a 10,000-year-old domesticated plant in the Americas. Proceedings of the National Academy of Sciences of the United States of America 102: 1831518320.
Fulton TM, Van der Hoeven R, Eannetta NT and Tanksley SD (2002) Identification, analysis, and utilization of conserved ortholog set markers for comparative genomics in higher plants. The Plant Cell 14: 14571467.
Hassell LR, Memmott F and Liere DG (2008) Grafting methods for watermelon production. HortScience 43: 16491677.
Jeffrey C (1967) Cucubitaceae. In: Milne-Redlead E and Polhill RM (eds) Flora of Tropical East Africa. London: Crown Agents, pp. 4753.
Jeffrey C (1980) A review of the Cucurbitaceae. Botanical Journal of the Linnean Society 81: 233247.
Kocyan A, Zhang L-B, Schäfer H and Renner SS (2007) A multi-locus chloroplast phylogeny for the Cucurbitaceae and its implications for character evolution and classification. Molecular Phylogenetics and Evolution 44: 553577.
Kousik CS, Levi A, Ling KS and Wechter WP (2008) Potential sources of resistance to cucurbit powdery mildew in US plant introductions of bottle gourd. HortScience 43: 13591364.
Lecoq H, Desbiez C, Wipf-Scheibel C and Girard M (2003) Potential involvement of melon fruit in the long distance dissemination of cucurbit potyviruses. Plant Diseases 87: 955959.
Lee JM and Oda M (2003) Grafting of herbaceous vegetable and ornamental crops. Horticultural Reviews 28: 61124.
Levi A and Thomas CE (1999) An improved procedure for isolation of high quality DNA from watermelon and melon leaves. Cucurbit Genetics Cooperative Report 22: 4142.
Levi A, Thomas CE, Keinath AP and Wehner TC (2001) Genetic diversity among watermelon (Citrullus lanatus and Citrullus colocynthis) accessions. Genetic Resources and Crop Evolution 48: 559566.
Levi A, Thomas CE, Trebitsh T, Salman A, King J, Karalius J, Newman M, Reddy OUK, Xu Y and Zhang X (2006) An extended linkage map for watermelon based on SRAP, AFLP, SSR, ISSR, and RAPD markers. Journal of American Society of Horticultural Science 131: 393402.
Li G and Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theoretical and Applied Genetics 103: 455461.
Ling K and Levi A (2007) Sources of resistance to zucchini yellow mosaic virus in Lagenaria siceraria germplasm. HortScience 42: 11241126.
Mai WF and Abawi GS (1987) Interactions among root-knot nematodes and Fusarium wilt fungi on host plants. Annual Review of Phytopathology 25: 317338.
Miguel A, Maroto JV, San Bautista A, Baixauli C, Cebolla V, Pascual B, López S and Guardiola JL (2004) The grafting of triploid watermelon is an advantageous alternative to soil fumigation by methyl bromide for control of Fusarium wilt. Scientia Horticulturae 103: 917.
Morimoto Y, Maundu P, Kawase M, Fujimaki H and Morishima H (2006) RAPD polymorphism of the white-flowered gourd Lagenaria siceraria (Molina) Standl. landraces and its wild relatives in Kenya. Genetic Resources and Crop Evolution 53: 963974.
Murata J and Ohara K (1936) Prevention of watermelon Fusarium wilt by grafting Lagenaria. Japanese Journal of Phytopathology 6: 183189 (in Japanese).
Nei M and Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America 76: 52695273.
Perkins-Veazie PM, Zhang X, Lu G and Huan J (2007) Grafting increases lycopene in seedless watermelon. HortScience 42: 959.
Renner SS and Schaefer H (2008) Phylogenetics of Cucumis (Cucurbitaceae) as understood in 2008. In: Pitrat M (ed.) Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae, Avignon, France, pp. 5358.
Richardson JB III (1972) The Pre-Columbian distribution of the bottle gourd (Lagenaria siceraria): a re-evaluation. Economic Botany 26: 265273.
Rohlf FJ (1993) NTSYS-PC Numerical Taxonomy and Multivariate Analysis System, Version 2.00. Setauket, NY: Exter Publishing, Ltd..
Sakata Y, Takayoshi O and Mitsuhiro S (2007) The history and present state of the grafting of cucurbitaceous vegetables in Japan. Acta Horticultrae 731: 159170.
SAS Institute, (2002) SAS/STAT User's Guide, Version 9.1. Cary, NC: South Institute.
Sato T and Ito K (1962) Fusarium spp. isolated from bottle gourd grafted watermelon. Annals of the Phytopathological Society of Japan 27: 252 (in Japanese).
Simmons AM and Levi A (2002) Sources of whitefly (Homoptera: Aleyrodidae) resistance in Citrullus for the improvement of cultivated watermelon. HortScience 37: 581584.
Taylor M, Bruton BD, Fish WW and Roberts W (2006) Cost benefit analyses of using grafted watermelons for disease control and the fresh-cut market. In: Holms J (ed.) Cucurbitaceae 2006, Asheville, NC, pp. 277285.
Thies JA and Fery RL (1998) Modified expression of the N gene for southern root-knot nematode resistance in pepper at high soil temperatures. Journal of American Society of Horticultural Science 123: 10121015.
Thies JA and Levi A (2003) Resistance of watermelon (Citrullus spp.) germplasm to the peanut root-knot nematode (Meloidogyne arenaria race 1). Hortscience 38: 14171421.
Thies JA and Levi A (2007) Characterization of watermelon (Citrullus lanatus Var. citroides) germplasm for resistance to root-knot nematodes. HortScience 42: 15301533.
Thies JA, Merrill SB and Corley EL Jr (2002) Red food coloring stain: new, safer procedures for staining nematodes in roots and egg masses on root surfaces. Journal of Nematology 34: 179181.
Whitaker TW (1972) The Pre-Columbian distribution of the bottle gourd (Lagenaria siceraria): a re-evaluation. Economic Botany 26: 265273.
Widjaja EA and Reyes MEC (1993) Lagenaria siceraria (Molina) Standley. In: Siemonsma JS and Piluek K (eds) Plant Resources of South-East Asia No. 8, Vegetables. Wageningen: Pudoc Scientific Publishers, pp. 190192.
Yetisir H, Çaliskan ME, Soylu S and Sakar M (2006) Some physiological and growth responses of watermelon [Citrullus lanatus (Thunb.) Matsum. and Nakai] grafted onto Lagenaria siceraria to flooding. Environmental and Experimental Botany 58: 18.
Yetisir H, Kurt F, Sari N and Tok FM (2007) Root stock potential of Turkish Lagenaria siceraria germplasm for watermelon: plant growth, graft compatibility, and resistance to Fusarium. Turkish Journal of Agriculture and Forestry 31: 381388.
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