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Genetic diversity in local barley accessions collected from different geographical regions of Tunisia

Published online by Cambridge University Press:  03 July 2009

M. A. Ould Med Mahmoud  and
S. Hamza
M. A. Ould Med Mahmoud
Affiliation:
Laboratory of Cereal Genetics and Plant Breeding, Institut National Agronomique de Tunisie, Avenue Charles Nicolle, 1082Tunis, Tunisia
S. Hamza*
Affiliation:
Laboratory of Cereal Genetics and Plant Breeding, Institut National Agronomique de Tunisie, Avenue Charles Nicolle, 1082Tunis, Tunisia
*
*Corresponding author. E-mail: hamza.sonia07@yahoo.fr
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Abstract

To assess the genetic diversity among Tunisian local barley, a set of 120 barley accessions representing five distinct geographical regions (North-West, Littoral, South, Jerba and Kerkennah Islands) was characterized with 20 simple sequence repeats (SSR) and 8 expressed sequence tag (EST)-SSR markers. The 28 loci revealed a total of 98 alleles, with an average of 3.76 alleles per locus (range 2–10). Gene diversity averaged 0.50 (range 0.09–0.84). Partitioning analysis of genetic diversity showed that about 95% of the total variation was within regions and no geographical differentiation could be found except for the North-West population. Similarly, neighbour joining clustering of the genotypes did not indicate any clear pattern of division among the barley accessions based on geographical origin. These results may reflect the impact of seed exchange between farmers which is likely to limit highlighting favourable alleles due to local adaptation.

Keywords

barleygenetic diversitymicrosatellitespopulation differentiationseed exchangeTunisia
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 2 , August 2009 , pp. 169 - 176
Copyright
Copyright © NIAB 2009

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