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Genetic diversity in Vicia faba L. populations cultivated in Tunisia revealed by simple sequence repeat analysis

  • Yassine Yahia (a1), Hédia Hannachi (a1), Antonio Jose Monforte (a2), James Cockram (a3), Mohamed Loumerem (a1), Belkacem Zarouri (a4) and Ali Ferchichi (a1)...
Abstract

Faba bean (Vicia faba L.) is one of the most important legumes in the world. Little is known about the genetic resources of faba bean in Southern Tunisia. In the present study, genetic diversity within Tunisian faba bean germplasms was investigated using 16 simple sequence repeat markers. In total, 50 alleles were detected. The number of alleles per marker ranged from 2 to 6, with an average of 3. Genetic diversity and polymorphism information content values averaged, respectively, 0.43 (range 0.34–0.51) and 0.36 (range 0.28–0.43). The mean heterozygosity value was 0.27. A model-based structure analysis based on neighbour-joining tree and factorial correspondence analysis revealed the presence of two subpopulations, consistent with the clustering based on genetic distance (GD). The overall F is value was 0.36, indicating the importance of selfing in these populations. Analysis of molecular variance revealed that the within-population genetic variance component was much higher than the between-population or between-subpopulation variance component. The genetic relationships based on Nei's GD revealed that AGD (Aguadulce) and SAG (Super Aguadulce) and TF1 and TF2 (Tafartassa-Gafsa) were the most closely related populations. Assessment of genetic variation within faba bean populations will be informative for the conservation of germplasms and the implementation of effective breeding programmes in Tunisia.

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* Corresponding author. E-mail: yahia.yassine@gmail.com
References
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Alvarez, MT, Sáenz de Miera, LE and Pérez de la Vega, M (1998) Genetic variation in common and runner bean of the Northern Meseta in Spain. Genetic Resources and Crop Evolution 45: 243251.
Bebeli, PJ and Kaltsikes, PJ (1993) New developments in varietal identification. In: van Gastel, AJG, Pagnotta, MA and Porceddu, E (eds) Seed Science and Technology. Aleppo: ICARDA, pp. 161172.
Belaid, Y, Chtourou- Chorbel, N, Marrakchi, M and Trifi-Farah, N (2006) Genetic diversity within and between populations of Lathyrus genus (Fabaceae) revealed by ISSR markers. Genetic Resources and Crop Evolution 53: 14131418.
Chaieb, N, Louis González, J, Lopez-Mesas, M, Bouslama, M and Valiente, M (2011) Polyphenol content and antioxidant capacity of thirteen faba bean (Vicia faba L.) genotypes cultivated in Tunisia. Food Research International 44: 970977.
Cipriani, G, Marazzo, MT, Marconi, R and Cimato, A (2002) Microsatellite markers isolated in olive (Olea europaea L.) are suitable for individual fingerprinting and reveal polymorphism within ancient cultivars. Theoretical and Applied Genetics 104: 223228.
Curley, J and Jung, G (2004) RAPD-based genetic relationships in Kentucky bluegrass: comparison of cultivars, interspecific hybrids, and plant introductions. Crop Science 44: 12991306.
Duc, G (1997) Faba bean (Vicia faba L.). Field Crops Research 53: 99109.
Engels, JMM, Ramanatha, RV, Brown, AHD and Jackson, MT (2002) Managing Plant Genetic Diversity. Rome: IPGRI.
Fatokun, CA, Menancio-Hautea, DI, Danesh, D and Young, ND (1992) Evidence for orthologous seed weight genes in cowpea and mung bean based on RFLP mapping. Genetics 132: 841846.
Gabriella, S, Angel a, RP and Domenico, P (1996) Distribution of allozyme variation in Vicia benghalensis (Leguminosae). Plant Systematics and Evolution 207: 99110.
Gong, Y, Xu, S, Mao, W, Li, Z, Hu, Q, Zhang, G and Ding, J (2011) Genetic diversity analysis of faba bean (Vicia faba L.) based on EST-SSR markers. Journal of Integrative Agriculture 10: 838844.
Gresta, F, Albertini, E, Raggi, L and Abbate, V (2009) A study of variability in the Sicilian faba bean landrace ‘Larga di Leonforte’. Genetic Resources and Crop Evolution 57: 523531.
Hallauer, AR and Miranda, JB (1988) Quantitative Genetics in Maize Breeding. 2nd edn. Ames, IA: Iowa State Univers ity Press.
Hamrick, JL and Godt, MJW (1990) Allozyme diversity in plant species. In: Brown, AHD, Clegg, MT, Kahler, AL and Weir, BS (eds) Plant Population Genetics, Breeding and Genetic Resources. Sunderland, MA: Sinauer Associates, pp. 145162.
Jin, Y, He, TH and Lu, BR (2006) Genetic spatial clustering: significant implications for conservation of wild soybean (Glycine soja: Fabaceae). Genetica 128: 4149.
Johnson, WC, Menendez, C, Nodari, R, Koinange, EMK, Magnusson, S, Singh, SP and Gepts, P (1996) Association of a seed weight factor with the phaseolin seed storage protein locus across genotypes, environments and genomes in PhaseolusVigna spp.: Sax (1923) revisited. Journal of Agricultural Genomics 2: Article 5, .
Karanja, J, Amugune, NO, Ininda, J, Kimatu, JN and Danson, JW (2009) Microsatellite analysis of the correlation between molecular and morphological traits in assorted maize inbred lines. African Crop Science Journal 17: 133144.
Kaur, S, Pembleton, LW, Cogan, NO, Savin, KW, Leonforte, T, Paull, J, Materne, M and Forster, JW (2012) Transcriptome sequencing of field pea and faba bean for discovery and validation of SSR genetic markers. BMC Genomics 13: 104115.
Khaldi, S and Zekri, S (2002) Production and Marketing of Legumes in Tunisia. In French. L'étude des légumineuses alimentaires dans les systèmes de production du Nord de la Tunisie. Ministry of Agriculture, Tunis, Tunisia, p. 92.
Kumar, S, Nei, M, Dudley, J and Tamura, K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Briefings in Bioinformatics 9: 299306.
Lawes, DA, Bond, DA and Poulsen, MH (1983) Classification, origin, breeding methods and objectives. In: Hebblethwaite, PD (ed.) The faba bean (Vicia faba L.), a basis for improvement. Butterworths, London, UK, pp. 2376.
Link, W, Dixkens, C, Singh, M, Schwall, M and Melchinger, AE (1995) Genetic diversity in European and Mediterranean faba bean germplasm revealed by RAPD markers. Theoretical Applied Genetics 90: 2732.
Link, W, Schill, B, Barbera, AC, Cubero, JI, Filippetti, A, Stringi, L, von Kittlitz, E and Melchinger, AE (1996) Comparison of intra- and inter-pool crosses in faba beans (Vicia faba L.). I. Hybrid performance and heterosis in Mediterranean and German environments. Plant Breeding 115: 352360.
Liu, K and Muse, SV (2005) Power Marker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21: 21282129.
Lopes, MS, Mendoca, D, Sefc, KM, Sabino Gil, F and Da Camara Machado, A (2004) Genetic evidence of intra-cultivar variability within Iberian olive cultivars. HortScience 39: 15621565.
Ma, Y, Bao, S, Yang, T, Hu, J, Quan, J, He, Y, Wang, X, Wan, Y, Sun, X, Jiang, J, Gong, C and Zong, X (2013) Genetic linkage map of Chinese native variety faba bean (Vicia faba L.) based on simple sequence repeat markers. Plant Breeding 132: 397400.
Mantzavinou, A, Bebeli, PJ and Kaltsikes, PJ (2005) Estimating genetic diversity in Greek durum wheat landraces with RAPD markers. Australian Journal Agricultural Research 56: 13551364.
Metayer, N (2004) Vicia faba L. breeding for sustainable agriculture in Europe-Identification of regional priorities and definition of target genotypes. GIE Feverole, Paris, France, p17.
Mohammadi, SA and Prasanna, BM (2003) Analysis of genetic diversity in crop plants: salient statistical tools and considerations. Crop Science 43: 12351248.
Nei, M (1983) Genetic polymorphism and the role of mutation in evolution. In: Nei, M and Koehn, R (eds) Evolution of Genes and Proteins. Sunderland, MA: Sinauer Associates, pp. 165190.
Ouji, A, Suso, MJ, Rouaissi, M, Abdellaoui, R and El Gazzah, M (2011 a) Genetic diversity of nine faba bean (Vicia faba L.) populations revealed by isozyme markers. Genes and Genomics 33: 3138.
Ouji, A, Rouaissi, M, Abdellaoui, R and El Gazzah, M (2011 b) The use of reproductive vigor descriptors in studying genetic variability in nine Tunisian faba bean (Vicia faba L.) populations. African Journal of Biotechnology 10: 896904.
Powell, W, Machray, GC and Provan, J (1996) Polymorphism revealed by simple sequence repeats. Trends in Plant Science 1: 215222.
Pradhan, A, Plummer, JA and Yan, G (2004) Comparison of morphological and molecular variation in seeds and seedlings of radish cultivars. Acta Horticulturae 637: 263270.
Ramakrishnan, AP, Meyer, SE, Waters, J, Stevens, MR, Coleman, CE and Fairbanks, DJ (2004) Correlation between molecular markers and adaptively significant genetic variation in Bromus tectorum (Poaceae), an inbreeding annual grass. American Journal of Botany 1: 797803.
Rohlf, FJ (1997) NTSYS-pc 2.1. Numerical Taxonomy and Multivariate Analysis System. Setauket, NY: Exeter Software.
Sheng, Y, Zheng, W, Pei, K and Ma, K (2005) Genetic variation within and among populations of a dominant desert tree Haloxylon ammodendron (Amaranthaceae) in China. Annals of Botany 96: 245252.
Sonnante, G, Piergiovanni, AR and Pignone, D (1997) Distribution of allozyme variation in Vicia benghalensis (Leguminosae) suggests the existence of two genepools. Plant Systematics and Evolution 207: 99110.
Sreelakshmi, Y, Gupta, S, Bodanapu, R, Chauhan, VS, Hanjabam, M, Thomas, S, Mohan, V, Sharma, S, Srinivasan, R and Sharma, R (2010) NEATTILL: a simplified procedure for nucleic acid extraction from arrayed tissue for TILLING and other high-throughput reverse genetic applications. Plant Methods 6: 3. DOI: 10.1186/1746-4811-6-3.
Schuelke, M (2000) An economic method for the fluorescent labelling of PCR fragments. Nature Biotechnology 18: 233234.
Terzopoulos, PJ and Bebeli, PJ (2008) Genetic diversity of Mediterranean faba bean (Vicia faba L.) with ISSR markers. Field Crops Research 108: 3944.
Terzopoulos, PJ, Kaltsikes, PJ and Bebeli, PJ (2003) Collection, evaluation and classification of Greek populations of faba bean (Vicia faba L.). Genetic Resources and Crop Evolution 50: 373381.
Terzopoulos, PJ, Kaltsikes, PJ and Bebeli, PJ (2008) Determining the sources of heterogeneity in Greek faba bean local populations. Field Crops Research 105: 124130.
Wang, HF, Zong, XX, Guan, JJ, Yang, T, Sun, XL, Ma, Y and Redden, R (2012) Genetic diversity and relationship of global faba bean (Vicia faba L.) germplasm revealed by ISSR markers. Theoretical Applied Genetics 124: 789797.
Yahia, Y, Guetat, A, Elfalleh, W, Ferchichi, A, Yahia, H and Loumerem, M (2012) Analysis of agromorphological diversity of southern Tunisia faba bean (Vicia faba L.) germplasm. African Journal of Biotechnology 11: 1191311924.
Yang, T, Bao, S, Ford, R, Jia, T, Guan, J, He, Y, Sun, X, Jiang, J, Hao, J, Zhang, X and Zong, X (2013) High-throughput novel microsatellite marker of faba bean via next generation sequencing. Molecules 18: 18441856.
Zeid, M, Schon, CC and Link, W (2003) Genetic diversity in recent elite faba bean lines using AFLP markers. Theoretical Applied Genetics 107: 13041314.
Zeid, M, Mitchell, S, Link, W, Carter, M, Nawar, A, Fulton, T and Kresovich, S (2009) Simple sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar ‘Giza 402’. Plant Breeding 128: 149155.
Zong, X, Ren, J, Guan, J, Wang, S, Liu, Q, Paull, JG and Redden, R (2010) Molecular variation among Chinese and global germplasm in spring faba bean areas. Plant Breeding 129: 508513.
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Plant Genetic Resources
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