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Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites

  • Padmavathi Nimmakayala (a1), Yan R. Tomason (a1) (a2), Jooha Jeong (a1), Gopinath Vajja (a1), Amnon Levi (a3), Paul Gibson (a2) (a4) and Umesh K. Reddy (a1)...

Thirty-eight melon accessions, which are of primary breeding importance in the Ukraine, were analysed for diversity. These collections represent a major non-US and non-western Europe source of melon germplasm that have not yet been subjected to molecular characterization. Molecular diversity was estimated based on a robust set of 465 polymorphisms gathered by amplified fragment length polymorphisms and simple sequence repeats (SSR). In this paper, we report 12 newly developed polymorphic SSR primer pairs, and their use for molecular characterization in the Ukrainian melon collections. Based on these polymorphisms, we estimated similarity indices that ranged from 0.70 to 1.00 among various accessions. The phylogenetic tree based on the similarity indices and a three-dimensional plot of the first three vectors of the principal component analysis corresponded fairly well with the existing three classical morphotypes namely aestivalis, europeus and hiemalis, under the convar Europeus, which is also known as adana. The polymorphisms generated in the current study, which are specific to the grouping of fruit types and days to maturity will be very useful for further genetic studies and marker-assisted selections.

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Arumuganathan K and Earle ED (1991) Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208218.
Connell JP, Pammi S, Iqbal MJ, Huizinga T and Reddy AS (1998) A high throughput procedure for capturing microsatellites from complex plant genomes. Plant Molecular Biology Reporter 16: 341349.
Danin-Poleg Y, Reis N, Tzuri G and Katzir N (2001) Development and characterization of microsatellite marker in Cucumis. Theoretical and Applied Genetics 102: 6172.
Decker-Walters DS, Chung S-M, Staub JE, Quemada HD and López- Sesé AI (2002) The origin and genetic affinities of wild population of melon (Cucumis melo, Cucurbitaceae) in North America. Plant Systematics and Evolution 233: 183197.
Dhillon NPS, Ranjana R, Singh Kuldeep, Eduardo I, Monforte AJ, Pitrat M, Dhillon NK and Singh PP (2007) Diversity among landraces of India snapmelon (Cucumis melo var. momordica). Genetic Resources and Crop Evolution 54: 12671283.
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783791.
Garcia-Mas J, Oliver M, Gómez-Paniagua H and De Vicente MC (2000) Comparing AFLP, RAPD and FLP markers for measuring genetic diversity in melon. Theoretical and Applied Genetics 101: 860864.
Garcia-Mas J, Monforte AJ and Arús P (2004) Phylogenetic relationships among Cucumis species based on the ribosomal internal transcribed spacer sequence and microsatellite markers. Plant Systematics and Evolution 248: 191203.
Gonzalo MJ, Oliver M, Garcia-Mas J, Monfort A, Dolcet-Sanjuan R, Katzir N, Arús P and Monforte AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theoretical and Applied Genetics 110: 802811.
Greuter W, Mcneil J, Barrie FR, Burdet HM, Demoulin V, Filgueiras TS, Nicolson DH, Silva PC, Skog JE, Trehane P, Turland NJ and Hawsworth DL (eds), (2002) The International Code of Botanical Nomenclature (Saint Louis Code) Sixteenth International Botanical Congress. St Louis, MS, July–August 1999 Regnum Vegetabile 138: 1–474. Königstein, Germany: Koeltz Scientific Books.
Jaccard P (1908) Nouvelles rescherches sur la distribution florale. Bulletin Société Vaudoise des Sciences Naturelles 44: 223270.
López-Sesé AI, Staub JE, Katzir N and Gómez-Guillamón ML (2002) Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127: 4151.
López-Sesé AI, Staub JE and Gómez-Guillamón ML (2003) Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. Theoretical and Applied Genetics 108: 4152.
Mliki A, Staub JE, Zhangyong S and Ghorbel A (2001) Genetic diversity in melon (Cucumis melo L.): an evaluation of African germplasm. Genetic Resources and Crop Evolution 48: 587597.
Monforte AJ, Garcia-Mas J and Arús P (2003) Genetic variability in melon based on microsatellite variation. Plant breeding 122: 153157.
Munger HM and Robinson RW (1991) Nomenclature of Cucumis melo L. Cucurbit Genetics Cooperative Report 14: 4344.
Nakata Eijiro, Staub JE, López-Sesé AI and Katzir N (2005) Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genetic Resources and Crop Evolution 52: 405419.
Oliver M, Garcia-Mas J, Morales M, Dolcet-Sanjuan R, Vicente M, Gómez H, Leeuwen H, Monfort A, Puigdomenech P and Arús P (2000) Proceedings of Cucurbitaceae 2000. In: Katzir N and Paris HS (eds) VII Eucarpia Meeting on Cucurbit Genetics and Breeding Acta Horticulturae. Israel: Ma'ale Ha Hamisha, pp. 375378.
Pangalo KI (1958) Melons [Dyni]. Kishinev (MD): State publisher [Gosudarstvennoe izdatel'stvo], pp. 350433.
Périn C, Hagen L, De Conto V, Katzir N, Danin-Poleg Y, Portnoy V, Baudracco-Arnas S, Chadoeuf J, Dogimont C and Pitrat M (2002) A reference map of Cucumis melo based on two recombinant inbred line populations. Theoretical and Applied Genetics 104: 10171034.
Pyzhenkov VI and Malinina MI (1994) Cucurbits (cucumber, melon). Flora of Cultivated Plants XXI. Moscow: Kolos, pp. 200288.
Reddy OUK, Pepper AE, Abdurakhmonov I, Saha S, Jenkins JN, Brooks T and BoelkYEl-Zik K (2001) New di-nucleotide and tri-nucleotide micorsatellite marker resources for cotton genome research. Journal of Cotton Science 5: 103113.
Ritschel PS, De Lima Lins TC, Tristan RL, Cortopassi Buso GS, Buso JA and Ferrira ME (2004) Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biology 4: 14712229.
Robinson RW and Decker-Walters DS (1997) Cucurbits. New York: CAB International, pp. 156200.
Rohlf FJ (1998) NTSYS-pc: numerical taxonomy and multivariate analysis system. Program Manual. New York: Exeter Software.
Saitou N and Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406425.
Sensoy S, Büyükalaca S and Abak K (2007) Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genetic Resources and Crop Evolution 54: 13511365.
Silberstein L, Kovalski I, Ruguo Huang, Anagnostou K, Jahn MMK and Perl-Treves R (1999) Molecular variation in melo (Cucumis melo L.) as revealed by RFLP and RAPD markers. Scientia Horticulturae 79: 101111.
Staub JE, Danin-Poleg Y, Fazio G, Horejsi T, Reis N and Katzir N (2000) Comparative analysis of cultivated melon groups (Cucumis melo L.) using random amplified polymorphic DNA and simple sequence repeat markers. Euphytica 115: 225241.
Staub JE, López-Sesé AI and Fanourakis N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136: 151166.
Stepansky A, Kovalski I and Perl-Treves R (1999) Intraspecific classification of melon (Cucumis melo L.) in view of their phenotypic and molecular variation. Plant Systematics and Evolution 217: 313332.
Szabó Z, Gyulai G, Humphreys M, Horváth L, Bittsánszky A, Lágler R and Heszky L (2005) Genetic variation of melon (Cucumis melo L.) compared to an extinct landrace from the middle ages (Hungry) 1. rDNA, SSR and SNP analysis of 47 cultivars. Euphytica 146: 8794.
Tanaka K, Nishitani A, Akashi Y, Sakata Y, Nishida H, Yoshino H and Kato K (2007) Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis. Euphytica 153: 233247.
Tomason YR (2002) Selection of melon lines with female flower type and morphological markers to create heterotic F1 hybrids PhD Thesis, Dnepropetrovsk State Agrarian University.
Urina OV, Pivovarov VF and Balashova NN (1998) Breeding and Seed Production of Cucurbit Crops in Russia. Moscow: VNIISSOK, pp. 400410.
Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Fritjers A, Pot J, Peleman J, Kuiper M and Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 44074414.
Yashiro K, Iwata H, Akashi Y, Tomita K, Kuzuya M, Tsumura Y and Kato K (2005) Genetic relationship among East and South Asian melon (Cucumis melo L.) revealed by AFLP analysis. Breeding Science 55: 197206.
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