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Molecular markers to assess genetic diversity of Gentiana lutea L. from the Ukrainian Carpathians

Published online by Cambridge University Press:  12 December 2014

Maryana Z. Mosula ,
Igor O. Andreev ,
Olena M. Bublyk ,
Vitaliy M. Mel'nyk ,
Iryna I. Konvalyuk ,
Nadiya M. Drobyk  and
Viktor A. Kunakh
Maryana Z. Mosula
Affiliation:
Volodymyr Hnatiuk Ternopil National Pedagogical University, M. Kryvonis street 2, Ternopil46027, Ukraine
Igor O. Andreev*
Affiliation:
Institute of Molecular Biology and Genetics NAS of Ukraine, Akad. Zabolotnogo street 150, Kyiv03680, Ukraine
Olena M. Bublyk
Affiliation:
Institute of Molecular Biology and Genetics NAS of Ukraine, Akad. Zabolotnogo street 150, Kyiv03680, Ukraine
Vitaliy M. Mel'nyk
Affiliation:
Institute of Molecular Biology and Genetics NAS of Ukraine, Akad. Zabolotnogo street 150, Kyiv03680, Ukraine
Iryna I. Konvalyuk
Affiliation:
Institute of Molecular Biology and Genetics NAS of Ukraine, Akad. Zabolotnogo street 150, Kyiv03680, Ukraine
Nadiya M. Drobyk
Affiliation:
Volodymyr Hnatiuk Ternopil National Pedagogical University, M. Kryvonis street 2, Ternopil46027, Ukraine
Viktor A. Kunakh
Affiliation:
Institute of Molecular Biology and Genetics NAS of Ukraine, Akad. Zabolotnogo street 150, Kyiv03680, Ukraine
*
*Corresponding author. E-mail: i.o.andreev@imbg.org.ua
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Abstract

The aim of the study was to develop the system of polymerase chain reaction (PCR)-based markers for the assessment of genetic diversity and population genetic studies of Gentiana lutea L. as well as to determine the utility of two indices of marker informativeness. The informativeness was determined for 40 PCR primers of different types (random amplified polymorphic DNA, inter simple sequence repeat, inter-retrotransposon amplified polymorphism, resistance gene analog polymorphism and conserved DNA-derived polymorphism markers) by evaluating discriminating power (DL) and resolving power (Rp) in a sample of 30 plants from two populations. Analysis of correlation between the index value and the number of differentiated pairs of genotypes in the given sample revealed that DL is more efficient than Rp; therefore, we selected primers based on the DL value. In total, 12 primers with the largest values of DL were chosen. Analysis of genetic relationship among 86 plants from six populations showed that the number of bands produced by the three of selected primers was sufficient to give average bootstrap support across six key nodes in the dendrogram higher than 85%, while using six of the primers resulted in average bootstrap value exceeding 99%. Thus, a minimal set of three to six selected primers are sufficient for a quick assessment of genetic diversity of G. lutea populations, depending on the sample size and degree of differentiation between populations, while the rest of the primers with DL values above 0.8 may be used for ecogenetic surveys. Preliminary results obtained with selected primers indicate the moderate level of genetic variation within the species and significant differentiation among individual populations.

Keywords

discriminating powergenetic diversityGentiana luteamolecular markersprimer informativenessresolving power
Type
Research Article
Information
Plant Genetic Resources , Volume 13 , Issue 3 , November 2015 , pp. 266 - 273
Copyright
Copyright © NIAB 2014 

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