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The new use of Sorghum bicolor-derived SSR markers to evaluate genetic diversity in 17 Australian Sorghum species

Published online by Cambridge University Press:  12 February 2007

Sally L. Dillon ,
Peter K. Lawrence  and
Robert J. Henry
Sally L. Dillon*
Affiliation:
Australian Tropical Crops and Forages Centre, Queensland Department of Primary Industries and Fisheries, LMB 1, Biloela, QLD 4715, Australia
Peter K. Lawrence
Affiliation:
Australian Tropical Crops and Forages Centre, Queensland Department of Primary Industries and Fisheries, LMB 1, Biloela, QLD 4715, Australia
Robert J. Henry
Affiliation:
Grain Foods CRC Limited, Centre for Plant Conservation Genetics, Southern Cross University, PO Box 157, Lismore, NSW 2480, Australia
*
*Corresponding author: E-mail:, sally.dillon@dpi.qld.gov.au
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Abstract

The Sorghum genus is extremely diverse both morphologically and geographically, however, relatively few of the 25 recognized species have been evaluated genetically. The apparent lack of basic knowledge pertaining to the levels of genetic diversity both within and between the 17 Australian wild species is a major obstacle to both their effective conservation and potential use in breeding programmes. Twelve Sorghum bicolor-derived simple sequence repeat (SSR) markers were evaluated for cross-species amplification in all 25 Sorghum species. The SSR markers were highly polymorphic, with diversity indices ranging from 0.59 to 0.99 with mean of 0.91. Five markers combined were able to differentiate 24 of the 25 Sorghum species, with intra-species polymorphism apparent. Sorghum bicolor-derived SSRs have proven to be an efficient source of markers for genetic diversity studies of the relatively poorly characterized Australian indigenous Sorghum species.

Keywords

cross-species amplificationgermplasm conservationmicrosatellitessorghum breeding
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 1 , April 2005 , pp. 19 - 28
Copyright
Copyright © The State of Queensland (Department of Primary Industries & Fisheries) 2005

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