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A panel of elite accessions of durum wheat (Triticum durum Desf.) suitable for association mapping studies

Published online by Cambridge University Press:  12 February 2007

Marco Maccaferri
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Maria Corinna Sanguineti
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Vincenzo Natoli
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
José Luis Araus Ortega
Affiliation:
Departament de Biologia Vegetal, Universitat de Barcelona, Barcelona, Spain
Moncef Ben Salem
Affiliation:
Tunisian National Institute of Agronomic Research, Tunis, Tunisia
Jordi Bort
Affiliation:
Departament de Biologia Vegetal, Universitat de Barcelona, Barcelona, Spain
Cynda Chenenaoui
Affiliation:
Tunisian National Institute of Agronomic Research, Tunis, Tunisia
Enzo De Ambrogio
Affiliation:
Società Produttori Sementi Bologna, Divisione Ricerca, Argelato (BO), Italy
Luis Garcia del Moral
Affiliation:
Dpto. Fisiologia Vegetal, University of Granada, Granada, Spain
Andrea De Montis
Affiliation:
Società Produttori Sementi Bologna, Divisione Ricerca, Argelato (BO), Italy
Ahmed El-Ahmed
Affiliation:
Plant Protection Department, Aleppo University, Aleppo, Syria
Fouad Maalouf
Affiliation:
Department of Plant Breeding, Lebanese Agricultural Research Institute, Bekaa, Lebanon
Hassan Machlab
Affiliation:
Department of Plant Breeding, Lebanese Agricultural Research Institute, Bekaa, Lebanon
Mark Moragues
Affiliation:
Area de Conreus Extensius Centre Udl-IRTA, Lleida, Spain
Jihan Motawaj
Affiliation:
ICARDA, Aleppo, Syria
Miloudi Nachit
Affiliation:
ICARDA, Aleppo, Syria
Nasserlehaq Nserallah
Affiliation:
CRRA-INRA, Settat, Morocco
Hassan Ouabbou
Affiliation:
CRRA-INRA, Settat, Morocco
Conxita Royo
Affiliation:
Area de Conreus Extensius Centre Udl-IRTA, Lleida, Spain
Roberto Tuberosa*
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
*
*Corresponding author. E-mail:roberto.tuberosa@unibo.it

Abstract

The effectiveness of association mapping (AM) based on linkage disequilibrium (LD) is currently being tested in a number of crops. An important prerequisite for the application of AM is the availability of collections of accessions with a suitable level of genetic variation for target traits and with limited spurious LD due to the presence of population structure. Herein, the results of a genome-wide molecular characterization of a collection of elite durum wheat accessions well-adapted to Mediterranean environments are presented. Ninety-seven highly polymorphic simple sequence repeats and 166 amplified fragment length polymorphism markers were used to characterize 189 durum accessions, mainly cultivars and advanced breeding lines. Genome-wide significant and sizeable LD indices at a centimorgan scale were observed, while LD mainly decayed within 10 cM. On the other hand, effects due to spurious LD were notably lower than those previously observed in a durum wheat collection sampling durum gene pools of more diverse origin. These results, coupled with the high level of genetic variability detected for a number of important morpho-physiological traits and their high heritability, indicate the suitability of this collection for AM studies targeting agronomically important traits.

Type
Research Article
Copyright
Copyright © NIAB 2006

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