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Proceedings of the Nutrition Society Proceedings of the Nutrition Society

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Effects of n-3 fatty acids on cartilage metabolism

Published online by Cambridge University Press:  28 July 2008

Clare L. Curtis ,
Sarah G. Rees ,
Joanna Cramp ,
Carl R. Flannery ,
Clare E. Hughes ,
Chris B. Little ,
Rhys Williams ,
Chris Wilson ,
Colin M. Dent  and
John L. Harwood
...Show all authors
Clare L. Curtis
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Sarah G. Rees
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Joanna Cramp
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Carl R. Flannery*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Clare E. Hughes
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Chris B. Little*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Rhys Williams
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
Chris Wilson
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
Colin M. Dent
Affiliation:
Department of Traumatic and Orthopaedic Surgery, University of Wales College of Medicine, Heath Park, Cardiff, CF14 4XW, UK
John L. Harwood
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
Bruce Caterson*
Affiliation:
Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3US, UK
*
*Present address: Wyeth Research, Cambridge, MA 02140, USA.
†Present address: Melbourne University Department of Paediatrics and Murdoch Children's Research Institute, Parkville 3052, Victoria, Australia.
†Present address: Melbourne University Department of Paediatrics and Murdoch Children's Research Institute, Parkville 3052, Victoria, Australia.
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Abstract

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Although the clinical benefits of dietary supplementation with n-3 polyunsaturated fatty acids (PUFA) has been recognised for a number of years, the molecular mechanisms by which particular PUFA affect metabolism of cells within the synovial joint tissues are not understood. This study set out to investigate how n-3 PUFA and other classes of fatty acids affect both degradative and inflammatory aspects of metabolism of articular cartilage chondrocytes using an in vitro model of cartilage degradation. Using well-established culture models, cartilage explants from normal bovine and human osteoarthritic cartilage were supplemented with either n-3 or n-6 PUFA, and cultures were subsequently treated with interleukin 1 to initiate catabolic processes that mimic cartilage degradation in arthritis. Results show that supplementation specifically with n-3 PUFA, but not n-6 PUFA, causes a decrease in both degradative and inflammatory aspects of chondrocyte metabolism, whilst having no effect on the normal tissue homeostasis. Collectively, our data provide evidence supporting dietary supplementation of n-3 PUFA, which in turn may have a beneficial effect of slowing and reducing inflammation in the pathogenesis of degenerative joint diseases in man.

Keywords

n-3 PUFACartilage metabolismChondrocytesDegenerative diseases
Type
Joint National Nurses Nutritional Group and Parenteral and Enteral Group of the British Dietetic Association Symposium on ‘Working in Partnership’
Information
Proceedings of the Nutrition Society , Volume 61 , Issue 3 , August 2002 , pp. 381 - 389
Copyright
Copyright © The Nutrition Society 2002

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