Hostname: page-component-76fb5796d-45l2p Total loading time: 0 Render date: 2024-04-25T20:28:57.325Z Has data issue: false hasContentIssue false
  • English
  • Français

Ambient ozone can induce plant defence reactions in tobacco

Published online by Cambridge University Press:  05 December 2011

Martina Schraudner ,
Ursula Graf ,
Christian Langebartels  and
Heinrich Sandermann Jr
Martina Schraudner
Affiliation:
GSF - Institut für Biochemische Pflanzenpathologie, D-85764 Oberschleißheim, Germany
Ursula Graf
Affiliation:
GSF - Institut für Biochemische Pflanzenpathologie, D-85764 Oberschleißheim, Germany
Christian Langebartels*
Affiliation:
GSF - Institut für Biochemische Pflanzenpathologie, D-85764 Oberschleißheim, Germany
Heinrich Sandermann Jr
Affiliation:
GSF - Institut für Biochemische Pflanzenpathologie, D-85764 Oberschleißheim, Germany
*
*Author for correspondence.
Get access

Synopsis

Typical phytopathological defence reactions of tobacco have been studied in response to ozone. After a single standard ozone pulse (5 h, 150 nl 1−1) β-l,3-glucanase was induced up to 40-fold in the sensitive tobacco cv. Bel W3, which developed necrotic lesions following the treatment. The activity was elevated 10-fold in the tolerant Bel B without concomitant injury. A decrease within 24 h occurred only in Bel B. Tyramine hydroxycinnamoyl-CoA transferase activity was induced 6- to 7-fold in both cultivars with a maximum after 5–8 h. Tyramine conjugates as the product of this reaction could not be isolated and were apparently rapidly integrated into the cell wall. Precultivation of Bel B plants in a greenhouse containing up to 70% of the outdoor ozone concentration resulted in a 4-fold induction of β-l,3-glucanase and a further increase after an administered ozone pulse. Pretreatment (30 nl 1−1 ozone, 7 h per day, 23 days) of sensitive Bel W3 caused increased injury and ethylene formation upon a subsequent ozone pulse. β-l,3-glucanase was induced to the same level as in non-pretreated control plants. In a comparison with other major air pollutants (SO2, NO2), only ozone induced the above reactions. However, a combined treatment of ozone and NO2 led to synergistic effects. These data indicate that ambient ozone is able to change the phytopathological disposition of plants. This finding may have practical significance for the usual plant experiments carried out in greenhouses with non-filtered air.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 55 - 61
Copyright
Copyright © Royal Society of Edinburgh 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Beimen, A., Witte, L. & Barz, W. 1992. Growth characteristics and elicitor-induced reactions of photosynthetically active and heterotrophic cell suspension cultures of Lycopersicon peruvianum (Mill.). Botanica Acta 105, 152–60.CrossRefGoogle Scholar
Boiler, T. 1993. Antimicrobial functions of the plant hydrolases, chitinase and β-1,3-glucanase. In Fritig, B. & Legrand, M. (Eds) Mechanisms of plant defense responses, pp. 391–400. Dordrecht: Kluwer Press.CrossRefGoogle Scholar
Booker, F. L., Fiscus, E. L. & Miller, J. E. 1991. Ozone-induced changes in soybean cell wall physiology. In Pell, E. & Steffen, K. (Eds) Active oxygen/oxidative stress and plant metabolism, pp. 229–32. Rockville: American Society of Plant Physiologists.Google Scholar
Ernst, D., Schraudner, M., Langebartels, C. & Sandermann, H. Jr 1992. Ozone induced changes of mRNA levels of β-1,3-glucanase, chitinase and pathogenensis-related protein lb in tobacco plants. Plant Molecular Biology 20, 673–82.CrossRefGoogle Scholar
Heagle, A. S. 1989. Ozone and crop yield. Annual Review of Phytopathology 27, 397–423.CrossRefGoogle Scholar
Heggestad, H. E. 1991. Origin of Bel-W3, Bel-C and Bel-B tobacco varieties and their use as indicators of ozone. Environmental Pollution 74, 264–91.CrossRefGoogle ScholarPubMed
Keefe, D., Hinz, U. & Meins, F. 1990. The effect of ethylene on the cell-type-specific and intracellular localization of β-1,3-glucanase and chitinase in tobacco leaves. Planta 182, 43–51.CrossRefGoogle ScholarPubMed
Langebartels, C., Kerner, K., Leonardi, S., Schraudner, M., Trost, M., Heller, W. & Sandermann, H. Jr, 1991. Biochemical plant responses to ozone: I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiology 95, 339–67.CrossRefGoogle ScholarPubMed
Negrel, J. & Jeandet, P. 1987. Metabolism of tyramine and feruloyltyramine in TMV inoculated leaves of Nicotiana tabacum. Phytochemistry 26, 2185–90.CrossRefGoogle Scholar
Negrel, J. & Martin, C. 1984. The biosynthesis of feruloyltyramine in Nicotiana tabacum. Phytochemistry 26, 2185–90.CrossRefGoogle Scholar
Rosemann, D., Heller, W., Sandermann, H. 1991. Biochemical plant responses to ozone: II. Induction of the stilbene biosynthesis in scots pine (Pinus sylvestris L.) seedlings. Plant Physiology 97, 1280–86.CrossRefGoogle ScholarPubMed
Schraudner, M., Ernst, D., Langebartels, C. & Sandermann, H. Jr 1992. Biochemical plant responses to ozone: III. Activation of the defense-related proteins β-1,3-glucanase and chitinase in tobacco leaves. Plant Physiology 99, 1321–28.CrossRefGoogle ScholarPubMed
Schraudner, M., Langebartels, C., Negrel, J. & Sandermann, H. Jr 1993. Plant defense reactions induced in tobacco by the air pollutant ozone. In Fritig, B. & Legrand, M. (Eds) Mechanisms of plant defense responses, pp. 286–90. Dordrecht: Kluwer Press.CrossRefGoogle Scholar
Steinberger, E. H. & Naveh, Z. 1982. Effects of recurring exposures to small ozone concentrations on Bel W3 tobacco plants. Agriculture and Environment 7, 255–63.CrossRefGoogle Scholar
Villegas, M. & Brodelius, P. E. 1990. Elicitor-induced hydroxycinnamoyl-CoA:tyramine hydroxycinnamoyltransferase in plant cell suspension cultures. Physiologia plantarum 78, 414–20.CrossRefGoogle Scholar
Yin, Z.-H., Langebartels, C. & Sandermann, H. Jr 1994. Specific induction of ethylene biosynthesis in tobacco plants by the air pollutant, ozone. Proceedings of the Royal Society of Edinburgh 102B, 127–30.Google Scholar