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Carbon Sources in Fruit Carbonate of Buglossoides arvensis and Consequences for 14C Dating

  • Kazem Zamanian (a1), Konstantin Pustovoytov (a2) (a3) and Yakov Kuzyakov (a1) (a4)
Abstract
Abstract

Fruit carbonate of Buglossoides arvensis (syn. Lithospermum arvense) is a valuable dating and paleoenvironmental proxy for late Quaternary deposits and cultural layers because CaCO3 in fruit is assumed to be accumulated from photosynthetic carbon (C). However, considering the uptake of HCO3 by roots from soil solution, the estimated age could be too old depending on the source of HCO3 allocated in fruit carbonate. Until now, no studies have assessed the contributions of photosynthetic and soil C to the fruit carbonate. To evaluate this, the allocation of photo-assimilated carbon and root uptake of HCO3 was examined by radiocarbon (14C) labeling and tracing. B. arvensis was grown in carbonate-free and carbonate-containing soils (sand and loess, respectively), where 14C was provided as (1) 14CO2 in the atmosphere (5 times shoot pulse labeling), or (2) Na2 14CO3 in soil solution (root-labeling; 5 times by injecting labeled solution into the soil) during one month of fruit development. Distinctly different patterns of 14C distribution in plant organs after root- and shoot labeling showed the ability of B. arvensis to take up HCO3 from soil solution. The highest 14C activity from root labeling was recovered in roots, followed by shoots, fruit organics, and fruit carbonate. In contrast, 14C activity after shoot labeling was the highest in shoots, followed by fruit organics, roots and fruit carbonate. Total photo-assimilated C incorporated via shoot labeling in loess-grown plants was 1.51 mg lower than in sand, reflecting the presence of dissolved carbonate (i.e. CaCO3) in loess. Loess carbonate dissolution and root-respired CO2 in soil solution are both sources of HCO3 for root uptake. Considering this dilution effect by carbonates, the total incorporated HCO3 comprised 0.15% of C in fruit carbonate after 10 hr of shoot labeling. However, if the incorporated HCO3 during 10 hr of shoot labeling is extrapolated for the whole month of fruit development (i.e. 420-hr photoperiod), fruit carbonate in loess-grown plants incorporated approximately 6.3% more HCO3 than in sand. Therefore, fruit carbonates from plants grown on calcareous soils may yield overestimated 14C ages around 500 yr because of a few percentage uptake of HCO3 by roots. However, the age overestimation because of HCO3 uptake becomes insignificant in fruits older than approximately 11,000 yr due to increasing uncertainties in age determination.

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*Corresponding author. Email: kzamani@gwdg.de.
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BD Amiro , LL Ewing . 1992. Physiological conditions and uptake of inorganic carbon-14 by plant roots. Environmental and Experimental Botany 32:203211.

H Brix . 1990. Uptake and photosynthetic utilization of sediment-derived carbon by Phragmites australis (Cav.) Trin. ex Steudel. Aquatic Botany 38:377389.

MD Cramer , ZF Gao , SH Lips . 1999. The influence of dissolved inorganic carbon in the rhizosphere on carbon and nitrogen metabolism in salinity-treated tomato plants. New Phytologist 142:441450.

MD Cramer , SH Lips . 1995. Enriched rhizosphere CO2 concentrations can ameliorate the influence of salinity on hydroponically grown tomato plants. Plant Physiology 94:425432.

MD Cramer , MB Richards . 1999. The effect of rhizosphere dissolved inorganic carbon on gas exchange characteristics and growth rates of tomato seedlings. Journal of Experimental Botany 50:7987.

CR Ford , N Wurzburger , RL Hendrick , RO Teskey . 2007. Soil DIC uptake and fixation in Pinus taeda seedlings and its C contribution to plant tissues and ectomycorrhizal fungi. Tree Physiology 27:375383.

M Gocke , K Pustovoytov , Y Kuzyakov . 2011. Carbonate recrystallization in root-free soil and rhizosphere of Triticum aestivum and Lolium perenne estimated by 14C labeling. Biogeochemistry 103:209222.

GA Goodfriend . 1987. Radiocarbon age anomalies in shell carbonate of land snails from semi-arid areas. Radiocarbon 29(2):159167.

AH Jahren , R Amundson , C Kendall , P Wigand . 2001. Paleoclimatic reconstruction using the correlation in δ18O of hackberry carbonate and environmental water, North America. Quaternary Research 56:252263.

Y Kuzyakov , E Shevtzova , K Pustovoytov . 2006. Carbonate re-crystallization in soil revealed by 14C labeling: Experiment, model and significance for paleo-environmental reconstructions. Geoderma 131:4558.

R Overstreet , S Ruben , TC Broyer . 1940. The absorption of bicarbonate ion by barley plants as indicated by studies with radioactive carbon. Proceedings of the National Academy of Sciences USA 26:688695.

JS Pigati , J Quade , TM Shahanan , CV Haynes Jr. 2004. Radiocarbon dating of minute gastropods and new constraints on the timing of late Quaternary spring-discharge deposits in southern Arizona, USA. Palaeogeography, Palaeoclimatology, Palaeoecology 204:3345.

JS Pigati , JA Rech , JC Nekola . 2010. Radiocarbon dating of small terrestrial gastropod shells in North America. Quaternary Geochronology 5:519532.

KE Pustovoytov , S Riehl , S Mittmann . 2004. Radiocarbon age of carbonate in fruits of Lithospermum from the early Bronze Age settlement of Hirbet ez-Zeraqōn (Jordan). Vegetation History and Archaeobotany 13:207212.

K Pustovoytov , S Riehl . 2006. Suitability of biogenic carbonate of Lithospermum fruits for 14C dating. Quaternary Research 65:508518.

K Pustovoytov , S Riehl , HH Hilger , E Schumacher . 2010. Oxygen isotopic composition of fruit carbonate in Lithospermeae and its potential for paleoclimate research in the Mediterranean. Global and Planetary Change 71:258268.

JAJ Stolwijk , KV Thimann . 1957. On the uptake of carbon dioxide and bicarbonate by roots, and its influence on growth. 1. Plant Physiololgy 32:513520.

A Viktor , MD Cramer . 2003. Variation in root-zone CO2 concentration modifies isotopic fractionation of carbon and nitrogen in tomato seedlings. New Phytologist 157:4554.

A Viktor , MD Cramer . 2005. The influence of root assimilated inorganic carbon on nitrogen acquisition/assimilation and carbon partitioning. New Phytologist 165:157169.

AH Vuorinen , EM Vapaavuori , S Lapinjoki . 1989. Time-course of uptake of dissolved inorganic carbon through willow roots in light and in darkness. Physiol. Plant 77:3338.

Y Wang , AH Jahren , R Amundson . 1997. Potential for 14C dating of biogenic carbonate in cackberry (Celtis) endocarps. Quaternary Research 47:337343.

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Radiocarbon
  • ISSN: 0033-8222
  • EISSN: 1945-5755
  • URL: /core/journals/radiocarbon
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