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Nonsense-mediated decay mutants do not affect programmed −1 frameshifting

  • LAURE BIDOU (a1), GUILLAUME STAHL (a2), ISABELLE HATIN (a1), OLIVIER NAMY (a1), JEAN-PIERRE ROUSSET (a1) and PHILIP J. FARABAUGH (a2)
  • Published online: 01 July 2000
Abstract

Sequences in certain mRNAs program the ribosome to undergo a noncanonical translation event, translational frameshifting, translational hopping, or termination readthrough. These sequences are termed recoding sites, because they cause the ribosome to change temporarily its coding rules. Cis and trans-acting factors sensitively modulate the efficiency of recoding events. In an attempt to quantitate the effect of these factors we have developed a dual-reporter vector using the lacZ and luc genes to directly measure recoding efficiency. We were able to confirm the effect of several factors that modulate frameshift or readthrough efficiency at a variety of sites. Surprisingly, we were not able to confirm that the complex of factors termed the surveillance complex regulates translational frameshifting. This complex regulates degradation of nonsense codon-containing mRNAs and we confirm that it also affects the efficiency of nonsense suppression. Our data suggest that the surveillance complex is not a general regulator of translational accuracy, but that its role is closely tied to the translational termination and initiation processes.

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Corresponding author
Reprint requests to: Philip J. Farabaugh, Department of Biological Sciences and Program in Molecular and Cell Biology, University of Maryland, Baltimore County, Baltimore, Maryland 21250, USA; e-mail: farabaug@umbc.edu.
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RNA
  • ISSN: 1355-8382
  • EISSN: 1469-9001
  • URL: /core/journals/rna
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