Skip to main content
×
Home
    • Aa
    • Aa

The identification and characterization of a novel splicing protein, Isy1p, of Saccharomyces cerevisiae

  • IAN DIX (a1), CAROLINE RUSSELL (a1), SIGAL BEN YEHUDA (a2), MARTIN KUPIEC (a2) and JEAN D. BEGGS (a1)
  • Published online: 01 March 1999
Abstract

We have identified a novel splicing factor, Isy1p, through two-hybrid screens for interacting proteins involved in nuclear pre-mRNA splicing. Isy1p was tagged and demonstrated to be part of the splicing machinery, associated with spliceosomes throughout the splicing reactions. At least a portion of the Isy1 protein population is associated with snRNAs; low levels of U5 and U6 snRNAs are coimmunoprecipitated specifically with Isy1p. When the ISY1 gene was knocked out, no defect in vegetative growth was observed. Using a sensitive in vivo splicing assay, however, we observed lower splicing efficiency in the isy1 null mutant compared to wild-type, indicating that Isy1p is important in the optimization of splicing.

Copyright
Corresponding author
Reprint requests to: Jean D. Beggs, Institute of Cell and Molecular Biology, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, United Kingdom; e-mail: jbeggs@ed.ac.uk.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

RNA
  • ISSN: 1355-8382
  • EISSN: 1469-9001
  • URL: /core/journals/rna
Please enter your name
Please enter a valid email address
Who would you like to send this to? *
×

Keywords: