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Morphology, anatomy and germination response of heteromorphic achenes of Anthemis chrysantha J. Gay (Asteraceae), a critically endangered species

  • Mayra Aguado (a1), Juan J. Martínez-Sánchez (a1), José Reig-Armiñana (a2), Francisco J. García-Breijo (a2) (a3), José A. Franco (a1) and María J. Vicente (a1)...

This study demonstrates that Anthemis chrysantha, a ‘Critically Endangered’ annual plant, produces two morphs of achenes: white and dark achenes, which differ in size, mass, anatomy and germination behaviour. Fresh white achenes germinated at all temperatures assayed from 10 to 25°C in both continuous darkness and 12-h photoperiod, ranging between 24% at 25°C in darkness and 89% at 12/20°C in light, whereas fresh dark achenes did not germinate under any temperature or light conditions. To identify differences in dormancy type between the two morphs, germination of dry-stored achenes, and achenes stratified at 5 or 25°C for 2 months were tested in both darkness and light at 5, 15 and 12/20°C for dry-stored and warm-stratified (25°C) achenes; and at 15, 25 and 12/20°C for cold-stratified (5°C) achenes. Of the white achenes, 90% germinated during the cold stratification period. In general, dry storage and warm stratification did not increase germination compared to fresh achenes. However, dark achenes did not germinate under any conditions. Dark achene dormancy was only broken by mechanical scarification or by excising the embryo (germination reached 71%). An anatomical study showed that the mesocarp of dark achenes had no intercellular spaces and was much thicker and stronger than that of white achenes, making the entry of water difficult, and also preventing germination by mechanical restriction. This study demonstrated that dormancy in the dark achenes is likely caused by the thickness of their pericarp, physically impeding germination and hampering imbibition of water.

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J.M. Baskin and C.C. Baskin (2004) A classification system for seed dormancy. Seed Science Research 14, 116.

K. Beneke , I. von Teichman , M.W. van Rooyen and G.K. Theron (1992) Fruit polymorphism in ephemeral species of Naquamaland. I. Anatomic differences between polymorphic diaspores of two Dimorphotheca species. South African Journal of Botany 58, 448455.

K. Beneke , M.W. van Rooyen , G.K. Theron and H.A. van de Venter (1993) Fruit polymorphism in ephemeral species of Naquamaland. III. Germination differences between polymorphic diaspores. Journal of Arid Environments 24, 333344.

M. Brändel (2004) Dormancy and germination of heteromorphic achenes of Bidens frondosa. Flora 199, 228233.

J.L. Brenchley and R.J. Probert (1998) Seed germination responses to some environmental factors in the sea grass Zostera capricorni from eastern Australia. Aquatic Botany 62, 177188.

D.F. Greene and E.A. Johnson (1989) A model of wind dispersal of winged or plumed seeds. Ecology 70, 339347.

Y. Gutterman (1993) Seed germination in desert plants. Adaptations of desert organisms. Berlin, Springer-Verlag.

E. Imbert (2002) Ecological consequences and ontogeny of seed heteromorphism. Perspectives in Plant Ecology, Evolution and Systematics 5, 1336.

E. Imbert , J. Escarré and J. Lepart (1996) Achene dimorphism and among-population variation in Crepis sancta (Asteraceae). International Journal of Plant Sciences 157, 309315.

E. Imbert , J. Escarré and J. Lepart (1999) Differentiation among populations for life history, morphology, head traits, and achene morph proportions in the heterocarpic species Crepis sancta (L.) Bornm. (Asteraceae). International Journal of Plant Sciences 160, 543552.

M.A. Khan and S. Gulzar (2003) Light, salinity, and temperature effects on the seed germination of perennial grasses. American Journal of Botany 90, 131134.

A. Kreitschitz and J. Vallés (2007) Achene morphology and slime structure in some taxa of Artemisia L. and Neopallasia L. (Asteraceae). Flora 202, 570580.

P.B. McEvoy (1984) Dormancy and dispersal in dimorphic achenes of tansy ragwort, Senecio jacobaea L. (Compositae). Oecologia 61, 160168.

Y. Mohamed-Yasseen , S.A. Barringer , W.A. Splittstoesser and S. Costanza (1994) The role of seed coat in seed viability. Botanical Review 60, 426439.

C. Oberprieler (2001) Phylogenetic relationships in Anthemis L. (Compositae, Anthemideae) based on nrDNA ITS sequence variation. Taxon 50, 745762.

K.K. Pandey (1960) Evolution of gametophyte and sporophyte systems of self-incompatibility in angiosperms. Evolution 14, 98115.

R. Porras and J.M. Muñoz (2000) Achene heteromorphism in the cleistogamous species Centaurea melitensis. Acta Oecologica 21, 231243.

I. Rashid , Z. Reshi , R.R. Allaie and B.A. Wafai (2007) Germination ecology of invasive alien Anthemis cotula helps it synchronise its successful recruitment with favourable habitat conditions. Annals of Applied Biology 150, 361369.

O.J. Rocha (1996) The effects of achene heteromorphism on the dispersal capacity of Bidens pilosa. International Journal of Plant Sciences 157, 316322.

W. Schütz , P. Milberg and B.B. Lamont (2002) Seed dormancy, after-ripening and light requirements of four annual Asteraceae in south-western Australia. Annals of Botany 90, 707714.

A.R. Spurr (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructure Research 26, 3143.

H.Z. Sun , J.J. Lu , D.Y. Tan , J.M. Baskin and C.C. Baskin (2009) Dormancy and germination characteristics of the trimorphic achenes of Garhadiolus papposus (Asteraceae), an annual ephemeral from the Junggar Desert, China. South African Journal of Botany 75, 537545.

B.D. Tanowitz , P.F. Salopek and B.E. Mahall (1987) Differential germination of ray and disc achenes in Hemizonia increscens (Asteraceae). American Journal of Botany 74, 303312.

D.L. Venable (1985) The evolutionary ecology of seed heteromorphism. American Naturalist 126, 577595.

D.L. Venable , A. Burquez , G. Corral , E. Morales and F. Espinosa (1987) The ecology of seed heteromorphism in Heterosperma pinnatum in central Mexico. Ecology 68, 6576.

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Seed Science Research
  • ISSN: 0960-2585
  • EISSN: 1475-2735
  • URL: /core/journals/seed-science-research
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