Skip to main content

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
Cancel
×
×
Home
  • Home
Article
  • Cited by 7
  • Cited by
    Crossref Citations
    Crossref logo
    This article has been cited by the following publications. This list is generated based on data provided by CrossRef.
    Li, Shuo Jia, Zirui Chen, Xiaoli Geng, Xuyun and Sun, Jinsheng 2015. Identification and characterization of the cDNAs encoding the two subunits of Chinese mitten crab (Eriocheir sinensis) calcineurin: Their implications in stress and immune response. Fish & Shellfish Immunology, Vol. 43, Issue. 1, p. 91.
    Nakazawa, Ayami Usuda, Nobuteru Matsui, Toshifumi Hanai, Toru Matsushita, Sachio Arai, Hiroyuki Sasaki, Hidetada and Higuchi, Susumu 2001. Localization of Calcineurin in the Mature and Developing Retina. Journal of Histochemistry & Cytochemistry, Vol. 49, Issue. 2, p. 187.
    Muzzio, I A. Talk, A C. and Matzel, L D. 1998. Intracellular Ca2+ and adaptation of voltage responses to light in Hermissenda photoreceptors. NeuroReport, Vol. 9, Issue. 7, p. 1625.
    Dorlöchter, Marlies and Stieve, Hennig 1997. The Limulus ventral photoreceptor: Light response and the role of calcium in a classic preparation. Progress in Neurobiology, Vol. 53, Issue. 4, p. 451.
    Edwards, Samuel C. O'Day, Peter M. and Herrera, Desiree C. 1996. Characterization of protein phosphatases type 1 and type 2A in Limulus nervous tissue: Their light regulation in the lateral eye and evidence of involvement in the photoresponse. Visual Neuroscience, Vol. 13, Issue. 01, p. 73.
    Calman, B.G Andrews, A.W Rissler, H.M Edwards, S.C and Battelle, B.-A 1996. Calcium/calmodulin-dependent protein kinase II and arrestin phosphorylation in Limulus eyes. Journal of Photochemistry and Photobiology B: Biology, Vol. 35, Issue. 1-2, p. 33.
    Kass, Leonard and Bray, William O 1996. Kinetic model for phototransduction and G-protein enzyme cascade: understanding quantal bumps during inhibition of CaM-KII or PP2B. Journal of Photochemistry and Photobiology B: Biology, Vol. 35, Issue. 1-2, p. 105.
    ×
  • Get access
    Add to cart USD35.00
    Check if you have access via personal or institutional login
    Log in Register Recommend to librarian

Characterization of a calcium/calmodulin-dependent protein phosphatase in the Limulus nervous tissue and its light regulation in the lateral eye

  • D. Z. Ellis (a1) and S. C. Edwards (a1)
Abstract

Calcium (Ca2+) plays an integral role in the light response of the photoreceptors in both vertebrate and invertebrate organisms. In the ventral eye of the horseshoe crab, Limulus polyphemus, a flash of light delivered to a dark-adapted photoreceptor stimulates a rapid rise in intracellular free calcium concentration ([Ca2+]i), which in turn mediates light adaptation. It has previously been demonstrated that in Limulus photoreceptors light, via Ca2+, activate s a calcium/calmodulin (Ca2+/CaM)-dependent protein kinase which increases the phosphorylation of arrestin. We now have identifie d biochemically, a calcium/calmodulin-dependent protein phosphatase (Ca2+/CaM PP ) in homogenates of the Limulus lateral and ventral eye, brain, and lateral optic nerve using as a substrate, a 32P-labeled peptide fragment of the regulatory subunit of cAMP-dependent protein kinase (RII). This protein phosphatase shares biochemical properties with calcineurin, a Ca2+/CaM-dependent protein phosphatase (type-2B). Its activity is enhanced by Ca2+, calmodulin and Mn2+; and is inhibited by mastoparan, a calmodulin antagonist, and a synthetic peptide corresponding to the autoinhibitory domain of mammalian calcineurin. Most importantly, light regulates the Ca2+/CaM PP activity in the lateral eye. While there is no difference in basal activity in long-term dark- or light-adapted preparations, Ca2+ enhances Ca2+/CaM PP activity only in long-term light-adapted eyes.

Copyright
COPYRIGHT: © Cambridge University Press 1994
References
Hide All
Aitken, A., Klee, C.B. & Cohen, P. (1984). The structure of the B subunit of calcineurin. European Journal of Biochemistry 139, 663–671.
Blumenthal, D.K., Takio, K., Hensen, R.S. & Krebs, E.G. (1986). Dephosphorylation of cAMP-dependent protein kinase regulatory subunit (type II) by calmodulin-dependent protein phosphatase. Journal of Biological Chemistry 261, 8140–8145.
Bolsover, S.R. & Brown, J.E. (1985). Calcium ion, an intracellular messenger of light-adaptation, also participates in excitation of Limulus photoreceptor. Journal of Physiology 364, 381–391.
Bradford, M.M. (1976). A rapid and sensitive method for the quanti-tation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 131, 248–254.
Brown, J.E. & Blinks, J.R. (1974). Changes in intracellular free calcium concentrations during illumination of invertebrate photoreceptors. Detection with aequorin. Journal of General Physiology 73, 219–243.
Brown, J.E. & Rubin, L.J. (1984). A direct demonstration that inositol-triphosphate induces an increase in intracellular calcium in Limulus photoreceptors. Biochemical and Biophysical Research Communications 125, 1137–1142.
Busse, R. & Mulsch, A. (1990). Calcium-dependent nitric oxide synthesis in endothelial cytosol is mediated by calmodulin. FEBS Letters 4, 133–134.
Calman, B.C., Andrews, A.W. & Battelle, B.A. (1992). The 46 kD protein in Limulus photoreceptors is phosphorylated by a calcium/calmodulin-dependent protein kinase. Investigative Ophthalmology and Visual Science 34, 807.
Cohen, P. (1991). Classification of protein-serine-threonine phosphatases: Identification and quantitation in cell extracts. Methods in Enzymology 201, 389–398.
Edwards, S.E., Wishart, A.C., Wiebe, E.M. & Battelle, B.A. (1989). Light-regulated proteins in Limulus ventral photoreceptor cells. Visual Neuroscience 3, 95–105.
Edwards, S.C. & Herrera, D.C. (1991). Protein phosphatases in the photoresponse in Limulus photoreceptor. Investigative Ophthalmology and Visual Science 32, 1008.
Edwards, S.E. (1993). Involvement of serine/threonine protein phosphatases in the photoresponse in Limulus photoreceptor. Advances in Protein Phosphatases 7, 529–542.
Goto, S., Matsukado, Y. & Mihara, Y. (1986). The distribution of calcineurin in rat brain and its relation to extrapyramidal system. Brain Research 397, 161–172.
Harrison, S.M. & Bers, D.M. (1987). The effects of temperature and ionic strength on the apparent Ca-affinity of EGTA and the analogous Ca-chelators BAPTA and dibromo-BAPTA. Biochimica et Biophysica Acta 925, 133–143.
Hashimoto, Y. & Soderling, T. (1988). Regulation of calcineurin by phosphorylation. Identification of the regulatory site phosphorylated by a Ca+/CaM-dependent protein kinase II and protein kinase C. The Journal of Biological Chemistry 264, 16524–16529.
Hashimoto, Y., Perrino, B.A. & Soderling, T.R. (1990). Identification of an autoinhibitory domain in calcineurin. Journal of Biological Chemistry 265, 1924–1927.
Hemmings, H.C. Jr., & Greengard, P. (1986). DARP-32, a dopamine-regulated phosphoprotein. Brain Research 69, 149–158.
Hendey, B., Klee, C. & Maxfield, F. (1992). Inhibition of neutro-phil chemokinesis on vitronectin by inhibitors of calcineurin. Science 258, 296–299.
Ingebritsen, T.S., Stewart, A.A. & Cohen, P. (1983). The protein phosphatases involved in cellular regulation 6. Measurement of type-I and type II protein phosphatases in extracts of mammalian tissue: An assessment of their physiological role. European Journal of Biochemistry 132, 297–304.
Kincaid, R.L., Vaughn, M., Osborne, J.C. Jr., & Tkachuk, V.A. (1982). Calcium-dependent interaction of 5-dimethylamino naphthalene-1-sulfonyl-calmodulin with cyclic nucleotide phospho-diesterase, calcineurin, and troponin-1. Journal of Biological Chemistry 257, 10638–10643.
Kincaid, R., Nightingale, M.S. & Martin, B. (1988). Characterization of a cDNA clone encoding the calmodulin-binding domain of mouse brain calcineurin. Proceedings of the National Academy of Sciences of the U.S.A. 85, 8983–8987.
King, M.M., Huang, C.Y., Chock, B.P., Nairn, A.C., Hemmings, H.C. Jr., Chan, K. & Greengard, P. (1984). Mammalian brain phosphoproteins as substrates for calcineurin. Journal of Biological Chemistry 259, 8080–8083.
Klee, C.B., Draetta, G.A. & Hubbard, M. (1988). Calcineurin. Advances in Enzymology 61, 149–200.
Klee, C.B. (1991). Concerted regulation of protein phosphorylation and dephosphorylation by calmodulin. Neurochemical Research 169, 1059–1065.
Levine, H. III, Smith, D.P., Whitney, M., Malicki, D.M., Dolph, P.J., Smith, G.F., Burkhart, W. & Zuker, C.S. (1991). Isolation of a novel visual-system-specific arrestin: An in vivo substrate for light-dependent phosphorylation. Mechanism for Development 33, 19–26.
Lisman, J.E. & Brown, J.E. (1975). Effects of intracellular ionophoretic injection of calcium buffers on light adaptation in Limulus ventral photoreceptors. Journal of General Physiology 66, 489–506.
Malinow, R., Schulman, H. & Tsien, R. (1989). Inhibition of post-synaptic PKC or CaM K II blocks induction but not expression of LTP. Science 245, 862–866.
Miller, J.L., Fox, D.A. & Litman, B.J. (1986). Amplification of phos-phodiesterase activation is greatly reduced by rhodopsin phosphorylation. Biochemistry 25, 4983–4988.
Novak-Hofer, I., Lemos, J.R., Villermain, M. & Levitan, I.B. (1985). Calcium and cyclic nucleotide-dependent protein kinases and their substrates in Aplysia nervous system. Journal of Biological Chemistry 269, 10283–10287.
Payne, R., Walz, B., Levy, S. & Fein, A. (1988). The localization of calcium release by inositol trisphosphate in Limulus photoreceptors and its control in negative feedback. Philosophy Transfer Research Society (London) 320, 359–379.
Roskoski, R. (1983). Assays of protein kinase. Methods in Enzymology 99, 3–6.
Shin, J., Richard, E.A. & Lisman, J.E. (1993). Ca2+ is an obligatory intermediate in the excitation cascade of Limulus photoreceptors. Neuron 11, 845–855.
Smith, W.C., Calman, B.G., Hutchinson, L., Greenberg, R.M., Donoso, L.A. & Battelle, B.A. (1993 a). Arrestin from Limulus lateral eyes: Cloning, expression, and localization. Investigative Ophthalmology and Visual Science 34, 807.
Smith, W.C., Eschweiler, A.W., Andrews, A., Greenberg, R.M. & Battelle, B.A. (1993 b). Circadian efferent input into Limulus retina stimulates the phosphorylation of a protein similar to the ninaC gene products of Drosophila. Society for Neuroscience Abstracts 19, 1199.
Steele, F.R., Washburn, T., Rieger, R. & O'Tousa, J. (1992). Drosophila retinal degeneration C (rdg C) encodes a novel serine/thre-onine protein phosphatase. Cell 69, 669–676.
Stewart, A.A., Ingebritsen, T.S., Manalan, A., Klee, C.D. & Cohen, P. (1982). Discovery of a calcium and calmodulin-dependent protein phosphatase. Probable identity with calcineurin (Cam-Bp80). FEBS Letters 137, 80–84.
Tallant, E.A. & Cheung, W.Y. (1983). Ontogeny of a CaM-dependent protein phosphatase. Biochemistry 22, 3630–3635.
Tallant, E.A. & Cheung, W.Y. (1984). Activation of bovine brain calmodulin-dependent protein phosphatase by limited trypsinization. Biochemistry 23, 973–979.
Warren, M.K. & Pierce, S.K. (1982). Two cell volume regulation systems in the Limulus myocardium: An interaction of ions and quaternary ammonium compounds. Biological Bulletin 163, 504–516.
Wlebe, E.M., Wishart, A.C., Edwards, S.C., & Battelle, B.A. (1989). Calcium/calmodulin stimulate phosphorylation of photo-receptor proteins in Limulus. Visual Neuroscience 3, 107–118.
Wolff, D.J. & Sved, D.W. (1985). The divalent cation dependence of bovine brain calmodulin-dependent phosphatase. Journal of Biological Chemistry 260, 4195–4202.
Wright, D., Noiman, E., Chock, P.B. & Chau, V. (1981). Fluoromet-ric assay for adenosine 3′ 5′-cyclic monophosphate-dependent protein kinaseand phosphoprotein phosphatase activities. Proceedings of the National Academy of Sciences of the U.S.A. 78, 6048–6050.
Yang, S.D., Tallant, E.A. & Cheung, W.Y. (1982). Calcineurin is a calmodulin-dependent protein phosphatase. Biochemical and Biophysical Research Communications 106, 1419–1425.
Recommend this journal

Email your librarian or administrator to recommend adding this journal to your organisation's collection.

Visual Neuroscience
  • ISSN: 0952-5238
  • EISSN: 1469-8714
  • URL: /core/journals/visual-neuroscience
Please enter your name
Please enter a valid email address
Who would you like to send this to? *

CAPTCHA *

Skip to the audio challenge
×

Keywords:

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 1 *
Loading metrics...

Abstract views

Total abstract views: 101 *
Loading metrics...

* Views captured on Cambridge Core between September 2016 - 12th June 2018. This data will be updated every 24 hours.

Cancel
Confirm
×