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ρ-Cymene Inhibits Growth and Induces Oxidative Stress in Rice Seedling Plants

  • Fengjuan Zhang (a1) (a2), Fengxin Chen (a1), Wanxue Liu (a2), Jianying Guo (a2) and Fanghao Wan (a2)...

ρ-Cymene was one of the major components of volatiles released by croftonweed. The allelopthy of ρ-cymene on the growth of upland rice seedlings was performed. Hydrogen peroxide generation, malondialdehyde (MDA) content, proline content, total ascorbate (ascorbate/dehydroascorbate), reduced/oxidized glutathione, and the levels of induction of antioxidant enzyme were studied in the seedlings of upland rice. ρ-Cymene inhibited the growth of upland rice seedlings. Exposure of upland rice seedlings to ρ-cymene increased levels of H2O2, MDA, and proline, indicating lipid peroxidation and induction of oxidative stress. Activities of the antioxidant enzymes superoxide dismutase, catalase, peroxidase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase were significantly elevated during the treatment period (7–15 d) compared with enzymes in the upland rice seedlings unexposed to ρ-cymene, thereby indicating the enhanced generation of reactive oxygen species (ROS) upon ρ-cymene exposure. These results suggest that activation of the antioxidant system by ρ-cymene led to the formation of ROS that resulted in cellular damage and decreased growth of upland rice seedlings.

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Ain-Lhout, F., Zunzunegui, F. A., Diaz Barradas, M. C., Tirado, R., Clavijio, A., and Novo, Garcia. F. 2001. Comparison of proline accumulation in two Mediterranean shrubs subjected to natural and experimental water deficit. Plant Soil. 230:175183.
Amaral, J. A. and Knowles, R. 1998. Inhibition of methane consumption in forest soils by monoterpenes. J. Chem. Ecol. 24:723734.
Apel, K. and Hirt, H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Ann. Review. Plant Biol. 55:373399.
Asada, K. 2006. Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol. 141:391396.
Asao, T., Kitazawa, H., Tomita, K., Suyama, K., Yamamoto, H., Hosoki, T., and Pramanik, M. H. R. 2004. Mitigation of cucumber autotoxicity in hydroponic culture using microbial strain. Sci. Hort. 99:207214.
Azevedo Neto, A. D., Prisco, J. T., Eneas-Filho, J., Braga de Abreu, C. E., and Gomes-Filho, E. 2006. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt tolerant and salt sensitive maize genotypes. Environ. Exp. Bot. 56:8794.
Bai, R., Zhao, X., Ma, F. W., and Li, C. Y. 2009. Identification and bioassay of allelopathic substances from the root exudates of Malus prunifolia . Allelopathy J. 23:477484.
Bais, H. P., Vepachedu, R., Gilroy, S., Callaway, R. M., and Vivanco, J. M. 2003. Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science. 301:13771380.
Baruah, N. C., Sarma, S., and Shara, R. P. 1994. Seed germination and growth inhibitory cadinenes from Eupatorium adenophorum Spreng. J. Chem. Ecol. 20:18851892.
Bates, L. S., Walderen, R. D., and Taere, I. D. 1973. Rapid determination of free proline for water stress studies. Plant Soil. 39:205207.
Batish, D. R., Singh, H. P., Kaur, S., Kohli, R. K., and Yadav, S. S. 2008. Caffeic acid affects early growth, and morphogenetic response of hypocotyl cuttings of mung bean (Phaseolus aureus). J. Plant Physiol. 165:297305.
Beauchamp, C. and FridovichI, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44:276286.
Becana, M., Dalton, D. A., Moran, J. F., Iturbe-Ormaetxe, I., Matamoros, M. A., and Rubio, M. C. 2000. Reactive oxygen species and antioxidants in legume nodules. Physiol. Plant. 109:372381.
Blokhina, O., Virolanen, E., and Fagerstedt, K. V. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot. 91:179194.
Cakmak, I. and Marschner, H. 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol. 98:12221227.
Dalton, D. A., Russell, S. A., Hanus, F. J., Pascoe, G. A., and Evans, H. J. 1986. Enzymatic reactions of ascorbate and glutathione that prevent peroxide damage in soybean root nodules. Proc. Natl. Acad. Sci. U. S. A. 83:38113815.
Dayan, F. E. and Watson, S. B. 2011. Plant cell membrane as a marker for light-dependent and light-independent herbicide mechanisms of action. Pestic. Biochem. Physiol. 101:182190.
Egley, G. H., Paul, R. N., Vaughn, K. C., and Duke, S. O. 1983. Role of peroxidase in the development of water-impermeable seed coats in Sida spinosa L. Planta. 157:224232.
Foyer, C. H. and Halliwell, B. 1976. Presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta. 133:2125.
Foyer, C. H., Lelandais, M., Edwards, E. A., and Mullineaux, P. M. 1991. The role of ascorbate in plants, interactions with photosynthesis and regulatory significance. Pp. 131144 in Pell, E. and Steffen, K., eds. Proceedings of the 6th Annual Penn State Symposium in Plant Physiology. Rockville, MD American Society of Plant Physiologists.
Foyer, C. H. and Noctor, G. 2003. Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol. Plant. 119:355364.
Foyer, C. H. and Noctor, G. 2009. Redox regulation in photosynthetic organisms: signaling, acclimation, and practical implications. Pp. 861905 in Buchanan, B., Dietz, K. J., and Pfannschmidt, T., eds. Antioxidants and Redox Signaling. Volume 11. New Rochelle, NY Mary Ann Liebert.
Foyer, C. H., Trebst, A., and Noctor, G. 2006. Protective and signalling functions of ascorbate, glutathione and tocopherol in chloroplasts. Pp. 241268 in Demmig-Adams, B., Adams, W. W., and Mattoo, A. K., eds. Advances in Photosynthesis and Respiration. Volume 21. Photoprotection, Photoinhibition, Gene Regulation, and Environment. Dordrecht, the Netherlands Kluwer Academic Publishers.
Grant, J. J. and Loake, G. J. 2000. Role of ROIs and cognate redox signaling in disease resistance. Plant Physiol. 124:2129.
Griffith, O. W. 1980. Determination of glutathione and glutathione disulphide using glutathione reductase and 2-vinyl pyridine. Anal. Biochem. 106:207212.
Hammondkosack, K. E. and Jones, J. D. G. 1996. Resistance gene-dependent plant defense responses. Plant Cell. 8:17731791.
Hare, P. D., Cress, W. A., and van Staden, J. 1998. Dissecting the roles of osmolyte accumulation during stress. Plant Cell Environ. 21:535553.
Heath, R. L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125:189198.
Hernandez, J. A., Jimenez, A., Mullineaux, P., and Sevilla, F. 2000. Tolerance of pea plants (Pisum sativum) to long-term salt stress is associated with induction of antioxidant defences. Plant Cell Environ. 23:853862.
Hierro, J. L. and Callaway, R. M. 2003. Allelopathy and exotic plant invasion. Plant Soil. 256:2939.
Jones, M. A. and Smirnoff, N. 2005. Reactive oxygen species in plant development and pathogen defence. Pp. 5386 in Smirnoff, N., ed. Antioxidants and Reactive Oxygen Species in Plants. Oxford, UK Blackwell Publishing.
Knight, K. S., Kurylo, J. S., Endress, A. G., Stewart, J. R., and Reich, P. B. 2007. Ecology and ecosystem impacts of common buckthorn (Rhamnus cathartica): a review. Biol. Invasions. 9:925937.
Lee, D. H., Kim, Y. S., and Lee, C. B. 2001. The inductive responses of the antioxidant enzymes by salt stress in rice (Oryza sativa L.). J. Plant Physiol. 158:737745.
Lowry, O. H., Rosebrough, N. T., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin–phenol reagent. J. Biol. Chem. 193:265275.
Meloni, D. A., Oliva, M. A., Martinez, C. A., and Cambraia, J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ. Exp. Bot. 49:6976.
Miake, C. and Asada, K. 1992. Thylakoid-bound ascorbate peroxidase in spinach chloroplasts and photoreduction of its primary oxidation product monodehydroascorbate radicals in thylakoids. Plant Cell Physiol. 33:541553.
Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22:867880.
Nishida, N., Tamotsu, S., Nagata, N., Saito, C., and Sakai, A. 2005. Allelopathic effects of volatile monoterpenoids produced by Salvia leucophylla: inhibition of cell proliferation and DNA synthesis in the root apical meristem of Brassica campestris seedlings. J. Chem. Ecol. 31:11871203.
Pala'-Pau'l, J., Pe'rez-Alonso, M. J., Velasco-Negueruela, A., and Sanz, J. 2002. Analysis by gas chromatography–mass spectrometry of the volatile components of Ageratina adenophora Spreng., growing in the Canary Islands. J. Chromatogr. A. 947:327331.
Romagni, J. G., Allen, S. N., and Dayan, F. E. 2000. Allelopathic effects of volatile cineoles on two weedy plant species. J. Chem. Ecol. 26:303313.
Romero-Romero, T., Sanchez-Nieto, S., San Juan-Badillo, A., Anaya, A. L., and Cruz-Ortega, R. 2005. Comparative effects of allelochemical and water stress in roots of Lycopersicon esculentum Mill. (Solanaceae). Plant Sci. 168:10591066.
Singh, H. P., Batish, D. R., Kaur, S., Arora, K., and Kohli, R. K. 2006. α-Pinene inhibits growth and induces oxidative stress in roots. Ann. Bot. 98:12611269.
Sofo, A., Dichio, B., Xiloynnis, C., and Masia, A. 2004. Effects of different irradiance levels on some antioxidant enzymes and on malondialdehyde content during rewatering in olive tree. Plant Sci. 166:293302.
Song, Q. S., Fu, J., Tang, J. W., Feng, Z. L., and Yang, C. R. 2000. Allelopathic potential of Eupatorium adenophorum Spreng. Acta Phytoecol. Sin. 24:362365.
Smirnoff, N. 1996. The function and metabolism of ascorbic acid in plants. Ann. Bot. 78:661669.
Sreenivasulu, N., Grimm, B., Wobus, U., and Weschke, W. 2000. Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedlings of fox-tail millet (Setaria italica). Physiol. Plant. 109:435442.
Testa, B. 1995. The Metabolism of Drugs and Other Xenobiotics. New York Academic Press. 471 p.
Tripathi, R. S., Singh, R. S., and Pai, J. P. N. 1981. Allelopathic potential of Eupatorium adenophorum, a dominant ruderal weed of Meghalaya. Proc. Indian Natl. Sci. Acad. Part B Biol. Sci. 47:458465.
Vaughn, S. F. and Spencer, G. F. 1993. Volatile monoterpenes as potential parent structures for new herbicides. Weed Sci. 41:114119.
Velikova, V., Yordanov, I., and Edreva, A. 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Plant Sci. 151:5966.
Wang, S. Y., Jiao, H. J., and Faust, M. 1991. Changes in ascorbate, glutathione, and related enzyme activities during thidiazuron-induced bud break of apple. Physiol. Plant. 82:231236.
Weir, T. L., Park, S-W., and Vivanco, J. M. 2004. Biochemical and physiological mechanisms mediated by allelochemicals. Curr. Opin. Plant Biol. 7:472479.
Yu, J. Q., Ye, S. F., Zhang, M. F., and Hu, W. H. 2003. Effects of root exudates and aqueous root extracts of cucumber (Cucumis sativus) and allelochemicals on photosynthesis and antioxidant enzymes in cucumber. Biochem. Syst. Ecol. 31:129139.
Yu, X. J., Yu, D., Lu, Z. J., and Ma, K. P. 2005. A new mechanism of invader success: exotic plant inhibits natural vegetation restoration by changing soil microbe community. Chin. Sci. Bull. 50:11051112.
Zhang, F. J., Guo, J. Y., Chen, F. X., Liu, W. X., and Wan, F. H. 2012. Identification of volatile compounds released by leaves of the invasive plant croftonweed (Ageratina adenophora, Compositae), and their inhibition of rice seedling growth. Weed Sci. 60:205211.
Zhang, J. H., Mao, Z. Q., Wang, L. Q., and Shu, H. R. 2007. Bioassay and identification of root exudates of three fruit tree species. J. Integr. Plant Biol. 49:257261.
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