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Mechanisms of Resistance to Glyphosate in a Ryegrass (Lolium Multiflorum) Biotype from Chile

Published online by Cambridge University Press:  20 January 2017

Paola Michitte ,
Rafael De Prado ,
Nelson Espinoza ,
Juan Pedro Ruiz-Santaella  and
Christian Gauvrit
Paola Michitte*
Affiliation:
Departamento de Química Agrícola y Edafología, Universidad de Córdoba. Campus de Rabanales, Edif. Marie Curie. 14071 Córdoba, España
Rafael De Prado
Affiliation:
Departamento de Química Agrícola y Edafología, Universidad de Córdoba. Campus de Rabanales, Edif. Marie Curie. 14071 Córdoba, España
Nelson Espinoza
Affiliation:
Centro Regional de Investigación, Carillanca, INIA. Km 10 Camino Cajón Vilcún. IX Región. Casilla 58-D. Temuco, Chile
Juan Pedro Ruiz-Santaella
Affiliation:
Departamento de Química Agrícola y Edafología, Universidad de Córdoba. Campus de Rabanales, Edif. Marie Curie. 14071 Córdoba, España
Christian Gauvrit
Affiliation:
UMR Biologie et Gestion des Adventices, INRA. 17 rue Sully. BP 86510, 21065 Dijon Cedex, France
*
Corresponding author's E-mail: pmenduca@yahoo.com
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Abstract

Glyphosate behavior was examined in Italian ryegrass plants from Chile that were sensitive (S) and resistant (R) to this herbicide. In order to explain the resistance to glyphosate, contact angles, spray retention, foliar uptake, herbicide translocation, and target enzyme activity were studied. Contact angles of glyphosate solutions at a field concentration were 40° to 45° on the abaxial surface of R leaves as compared to 70° on S. Glyphosate spray retention by R plants was 35% lower than by S plants. Glyphosate uptake by the abaxial leaf surface of R plants was about 40% lower than that of S plants. In addition, in the R plants more glyphosate migrated to the tip of the treated leaves. The target enzyme in R and S plants was sensitive to the herbicide. Based on these and previous results, it is concluded that resistance in this Italian ryegrass biotype results from lower spray retention, lower foliar uptake from the abaxial leaf surface, and altered translocation pattern. The decreases in spray retention and foliar uptake constitute new mechanisms of glyphosate resistance.

Keywords

GlyphosateItalian ryegrass Lolium multiflorum Lam. LOLMUSpray retentioncontact anglefoliar uptaketranslocation
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 55 , Issue 5 , October 2007 , pp. 435 - 440
Copyright
Copyright © Weed Science Society of America 

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