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Mechanisms of Resistance to Glyphosate in a Ryegrass (Lolium Multiflorum) Biotype from Chile

  • Paola Michitte (a1), Rafael De Prado (a1), Nelson Espinoza (a2), Juan Pedro Ruiz-Santaella (a1) and Christian Gauvrit (a3)...

Glyphosate behavior was examined in Italian ryegrass plants from Chile that were sensitive (S) and resistant (R) to this herbicide. In order to explain the resistance to glyphosate, contact angles, spray retention, foliar uptake, herbicide translocation, and target enzyme activity were studied. Contact angles of glyphosate solutions at a field concentration were 40° to 45° on the abaxial surface of R leaves as compared to 70° on S. Glyphosate spray retention by R plants was 35% lower than by S plants. Glyphosate uptake by the abaxial leaf surface of R plants was about 40% lower than that of S plants. In addition, in the R plants more glyphosate migrated to the tip of the treated leaves. The target enzyme in R and S plants was sensitive to the herbicide. Based on these and previous results, it is concluded that resistance in this Italian ryegrass biotype results from lower spray retention, lower foliar uptake from the abaxial leaf surface, and altered translocation pattern. The decreases in spray retention and foliar uptake constitute new mechanisms of glyphosate resistance.

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N. Amrhein , B. Deus , P. Gehrke , and H. C. Steinrucken 1980. The site of the inhibition of the shikimate pathway by glyphosate. II. Interference of glyphosate with chorismate formation in vivo and in vitro. Plant Physiol. 66:830834.

S. R. Baerson , D. J. Rodriguez , M. Tran , Y. Feng , N. A. Biest , and G. M. Dill 2002. Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol. 129:12651275.

G. E. Blackman , R. S. Bruce , and K. Holly 1958. Studies in the principles of toxicity. V. Interrelationships between specific differences in spray retention and se-lective phytotoxicity. J. Exp. Bot. 9:175205.

G. E. Fogg 1947. Quantitative studies of the wetting of leaves by water. Proc. Royal Soc. (London) Series B. 134:503522.

D. F. Lorraine-Colwill , S. B. Powles , T. R. Hawkes , P. H. Hollinshead , S. A. J. Warner , and C. Preston 2003. Investigations into the mechanism of glyphosate resistance in Lolium rigidum . Pestic. Biochem. Physiol. 74:6272.

J. Lydon and S. O. Duke 1988. Glyphosate induction of elevated levels of hydroxbenzoic acids in higher plants. J. Agric. Food Chem. 36:813818.

T. C. Mueller , J. H. Massey , R. M. Hayes , C. L. Main , and C. N. Stewart Jr. 2003. Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq.). J. Agric. Food Chem. 51:680684.

C. H. Ng , R. Wickneswari , S. Salmijah , Y. T. Teng , and B. S. Ismail 2003. Gene polymorphisms in glyphosate-resistant and -susceptible biotypes of Eleusine indica from Malaysia. Weed Res. 43:108115.

J. J. Schott , J. L. Dufour , and C. Gauvrit 1991. Effects of adjuvants on herbicidal action. III – Effects of petroleum and rapeseed oils on diclofop-methyl action on ryegrass. Agronomie. 11:2734.

A. M. Wakelin , D. F. Lorraine-Colwill , and C. Preston 2004. Glyphosate resistance in four different populations of Lolium rigidum is associated with reduced translocation of glyphosate to meristematic zones. Weed Res. 44:453459.

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Weed Science
  • ISSN: 0043-1745
  • EISSN: 1550-2759
  • URL: /core/journals/weed-science
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